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Genetic Improvement of Fungal Strains for Hyper Production of Ligno Cellulolytic Enzymes.

Sk.A. Hasib
ASO
Microbiology & Biochemistry Department, BJRI,Dhaka.

Abstract/ Executive Summary:

A number of stable mutants of RT – 9 have been developed through exposing to Gamma radiation for a longer period. Although the developed mutants were different in colour and appearance but their enzyme productivity in wheat bran medium did not exceed that of RT – 9. So the development of hyper enzyme producing mutants with different doses of radiation and chemicals are in progress.

Keywords: Thermomycys, Lanuginosus, Gamma radiation, Enzymes

Location: Central station, BJRI,Dhaka

Start Date: 01-07-1996

End Date: 01-07-1997

Program Area: Variety and Species

Commodity: Jute

Objectives:

Development of the known lignocellulolytic fungal strains for their enhanced enzyme production. Application of enzyme from the mutant strains for softening of jute / jute cuttings etc. to reduce non – cellulosic constituents and improve physical properties.

Methodology:

In 1996 a thermophilic fungus Thermomyces lanuginosus was subjected to Gamma radiation at AERE saver for mutation. Although after 3 successive generations several stable mutants were found in terms of colour and appearance, but enzyme productivity of these mutants in wheat bran media were not encouraging. Considering the outcome of survival and morphological changes of the fungus ( RT – 9) due to exposure to gamma radiation and to make up more genetic changes, the exposure time was set up for eight minutes for mutation.The exposed spore suspension was suitably dilluted and 0.5 ml of dilluted spore solution was incubated in PDA plates at 50degree centigrade . After incubation of 3 days only a few fungal mutants colony was visible. They were transferred to fresh PDA plates until they become stable ( up to successive generation ). Thus out of several developed mutants of RT-9. Only 2mutants were found and were designated as RT – 9 as – 9/1 and RT – 9 / 2.

Source of Information: Annual Technical Report 1997

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

A well known ligno-cellulolytic thermophilic fungus Thermomyces lanuginosus was exposed to Gamma radiation at AERE , savar Dhaka for mutation.

Knowledge Management: Report/Proceedings