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Research Detail

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AKLIMA KHATUN
Department of Zoology, Rajshahi University, Rajshahi-6205, Bangladesh

ATAUR RAHMAN KHAN
Department of Zoology, Rajshahi University, Rajshahi-6205, Bangladesh

M. NURUL ISLAM
Department of Zoology, Rajshahi University, Rajshahi-6205, Bangladesh

RAFIZA SULTANA
Department of Zoology, Rajshahi University, Rajshahi-6205, Bangladesh

Effects of the contact toxicity of Thevetia peruviana leaf extracts in petroleum spirit. ethyl acetate. acetone and methanol on three strains of Tribolium castaneum adults . viz. CR - 1. FSS- II and CTC- 12. have been investigated. The toxicity was in  the order: ethyl  acetate > acetone  > methanol  > petroleum spirit.

  Thevetia peruviana. Tribolium castaneum, Control, Insecticidal effect
  Insect Research laboratory, Department of Zoology, Rajshahi University
  
  
  Pest Management
  Insecticide

To identify the Insecticidal Effect of Thevetia Peruviana (Pers.) Schum Leaf Extract on Tribolium Castaneum (Herbst)

Three standard strains of T. cqstaneum, viz . CR-1, FSS-II and CTC - 12 were maintained on a standard mixture of whole wheat flour with powdered dry yeast ( 19 : 1) at 30 ± 2°C in the Insect Research laboratory, Department of Zoology, Rajshahi University. Leaves of T. peruviana were sun-dried and powdered in mortar and pestle, and then transferred to grinder to have fine dust. The active ingredients from leaf were extracted with petroleum spirit (pt. spt.), ethyl acetate (EtOAC), acetone and methanol (MeOH), and were preserved at 4°C for future use. Dried extracts were weighed and dissolved in the solvents to prepare the experimental .doses, e.g. 116.758, 77.839, 51.892 and 34.595 gf!..tl for Pt. Spt. extract; 14.285, 9.523, 6.349 and 4.232 gf!..tl for EtOAc extract; 15.634, 10.433, 6.948 and 4.632 g/ 1 for acetone extract and 54.494, 36.330, 24.230 and 16.146 g/ 1 for MeOH extract, which were applied topically to the adult beetles to determine their mortality at 24-,48- and 72 hr post-exposure. For each dose and strain 10 insects were used and the experiments were repeated thrice. Batches of control insects were treated with the solvents only. The mortalily of T. castaneum adults were corrected by Abbott's (1925) formula and were then subjected to probit analysis.

  J. Asiat. Soc. Bangladesh, Sci. 25( l) : 25-29, 1999
  
Funding Source:
  

The effect of T. peruviana leaf extract on the adults of T . castaneum. Results show that the ethyl acetate extract was most toxic to the beetles which was followed by acetone, methanol and petroleum spirit extracts respectively. The contact toxicity of the leaf extracts to the strains of T. castaneum could not be generalized. It was in the order CR­ l CTC-12 FSS-II for petroleum spirit, FSS-II CTC-12 CR-1 for ethyl acetate, CR-l FSS-Il CTC-12 for acetone and CTC-12 CR­ l FSS-II for methanol extracts respectively. The contact toxicity of the leaf, seed, fruit-pericarp, stembark and root of Amoora rohituka W. & A. using the same solvents and with the same strains of the flour beetle used in the present investigation. He observed the highest mortality for ethyl acetate leaf extracts which is similar to the present finding.

  Journal
  


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