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Environmentally Persistant Toxicant Arsenic Affects Uterus Grossly and Histologically

M. A. Wares
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh

M. A. Awal
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh

S. K. Das
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh

J. Alam
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The experiment was carried out to determine the gross and microscopic effects of arsenic on uterus of female Black Bengal Goats against control group in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202. A total of twelve mature female Black Bengal goats were studied, six from arsenic affected areas of Mymensingh district and the other six from Hill Tracts of Bangaldesh which are arsenic free. Gross parameters show slight variations in the morphology (color and shape) and biometry (size and weight) of uterus of arsenic affected Black Bengal goats, but this variation was statistically insignificant (p>0.05). For microscopic study, permanent slide was prepared by Mayer’s Hematoxylin and Eosin stain method. In microscopic level, there were significant variations in arsenic affected group of goats compared to the control group. Arsenic affected group of goats show thickening of uterine horn (p<0.01), uterine gland is lower in amount and diameter(p<0.01), cervical villi length was smaller (p<0.01), cervical wall thickness was higher (p<0.01). This work may be the tip of the iceberg representing the full extent of arsenic exposure to female genital tract.

Keywords: Goat, Reproductive tract, Arsenic, Effects, Microscopic

Location: BAU, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Goat

Objectives:

To determine the gross and microscopic effects of arsenic on uterus.

Methodology:

Collection of samples :A total of twelve mature female Black Bengal goats were used in this study. Six (two from Buror Char, two from Dhobaura, one from Fulpur and one from Haluaghat upa-zilla of Mymensingh) mature female Black Bengal goats were purchased for experiment being aware that the goats have been reared in those specific areas from their birth. Control group of goats (sx) were purchased from the arsenic free areas of Hill Tracts of Chittagong (Chakraborti et al. 2010).The goats were sacrificed by piercing carotid artery and uterus were collected immediately in the postgraduate laboratory, department of Anatomy and Histology, Bangladesh Agricultural University (BAU), Mymensingh. Gross and Histological Study Just after collection of female reproductive organ (uterus), the gross morphology (color and shape) and biometry (size and weight) were measured and recorded. For histological studies, the tissues were trimmed and then fixed in the “Bouin’s fluid” (Gridley, 1960), dehydrated in the series of asecnding grades of alcohol, cleared in three changes in xylene, filtrated in three grades of melting paraffin in the oven of 580 C and embedded in paraffin of 580 C melting temperature. Then the sections were cut into 6μ thickness using rotatory microtome (MIC 509, Euromex, Japan). The sections were stained using Mayer’s Hematoxylin and Eosin Formula (Luna, 1968). Photomicrograph and Statistical Analysis: The stuctures were observed by light microscope using convenient magnification, measurement of important structures was taken by the calibrated stage micrometer and photomicrograph was taken for better illustration using photomicrographic camera (Olympus PMC 35 Model). The recorded data were compiled and tabulated for statistical analysis by the help of Computer package SPSS 11. The mean was compared using One-way ANOVA Method.

Source of Information: Bangl. J. Vet. Med. (2013).11 (1):61-68 ISSN: 1729-7893 (Print) 2308-0922 (Online)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Gross parameters show slight variations in the morphology (color and shape) and biometry (size and weight) of uterus of arsenic affected Black Bengal goats, but this variation was statistically insignificant (p>0.05). For microscopic study, permanent slide was prepared by Mayer’s Hematoxylin and Eosin stain method. In microscopic level, there were significant variations in arsenic affected group of goats compared to the control group. Arsenic affected group of goats show thickening of uterine horn (p<0.01), uterine gland is lower in amount and diameter(p<0.01), cervical villi length was smaller (p<0.01), cervical wall thickness was higher (p<0.01). This work may be the tip of the iceberg representing the full extent of arsenic exposure to female genital tract.

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