Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Study of Environmental and Nutritional Factors on the Hatching and Development of Eggs of Haemonchus Contortus

F Khatun
Department of Parasitology, Faculty of Veterinary Science, Bangladesh AgriculturalUniversity, Mymensingh, Bangladesh

N Begum
Department of Parasitology, Faculty of Veterinary Science, Bangladesh AgriculturalUniversity, Mymensingh, Bangladesh

S Akter
Department of Parasitology, Faculty of Veterinary Science, Bangladesh AgriculturalUniversity, Mymensingh, Bangladesh

MMH Mondal
Department of Parasitology, Faculty of Veterinary Science, Bangladesh AgriculturalUniversity, Mymensingh, Bangladesh

Abstract/ Executive Summary:

To determine the in vitro effects of environmental and nutritional factors on the development and hatching of eggs of Haemonchus contortus, eggs were cultivated at different temperatures, humidity and pH, in phosphate buffered saline (PBS), tap water, or normal saline, with and without faecal material and soil. No development of eggs occurred at 4ºC up to 15 days. Hatching was maximum (40.7%) at 30ºC on Day 5. Maximum hatching (39.5%) was observed on Day 6 at pH 6 and hatching was minimum (3.3%) on Day 4 at pH 3. Hatching did not occur at pH 2. At a relative humidity of 80 - 90%, maximum hatching (42.2%) was recorded on Day 5. Light had no effect on development and hatching. Maximum proportion of eggs hatched in PBS containing faecal material (40.1%). It is suggested that PBS containing faecal material may be used for the incubation of eggs of Haemonchus contortus. Best results may be obtained at 30ºC, pH 6 and relative humidity 80 - 90%.

Keywords: Environmental and nutritional factors, Hatching and development of eggs, Haemonchus contortus

Location: BAU, Mymensingh

Start Date: 00-11-2011

End Date: 00-04-2012

Program Area: Pest Management

Commodity: Diseases

Objectives:

The present study was designed to find-

The effects of temperature, pH, humidity and light on the hatching of eggs and the development of larvae of Haemonchus contortus.
Suitable media for the incubation of eggs
Effects of nutrients (faecal material and soil) on the development and hatching of eggs

Methodology:

The study was carried out from November 2011 to April 2012. Abomasa of goats were collected from Kamal Ranjit Market of Bangladesh Agricultural University (BAU), Mymensingh. Female parasites were crushed using pestle and mortar containing phosphate buffered saline (PBS). Eggs were counted by modified McMaster technique (Thienpont et al., 1979) using McMaster counting chamber. Total number of eggs/mL = (Total eggs × 10)/0.3. Study of the effects of temperature: Eggs were incubated at 35°C, 30°C, 20°C, 10°C and 4°C. To achieve 4°C, the culture media were kept in a refrigerator. A counted number of eggs were suspended in a petridish containing PBS. The petridishes were kept in an aluminium tray covered with a thin layer of moist cotton to prevent the evaporation of culture media. They were incubated up to 7 days and examined regularly at 24 hour intervals using a dissecting microscope. A drop of culture media was examined to see the development of eggs and the larval stages (L1, L2 and L3). After 15 days observation, the culture media were kept at room temperature to record the further development as described by Soulsby (1982). Study of the effects of pH: To study the effects of pH, the pH of the culture media were adjusted to 2, 3, 4, 5, 6, 7, 8 and 9 by adding hydrochloric acid (HCl) into PBS drop by drop with the help of micropipette while stirring the media. The pH was detected by pH meter. Observations were made every 24 hours up to seven days using a dissecting microscope. Study of the effects of humidity: To determine the effects of humidity a counted number of eggs was mixed with PBS and incubated at 70 - 80% and 80 - 90% relative humidity at constant 30°C. Hygrometer was used to measure the relative humidity in the incubator. Humidity was controlled by adding water in the petridishes. Developmental stages were observed by dissecting microscope at 24 hour intervals up to six days. Study of the effects of light: The culture media with a counted number of eggs were covered with black cloth to exclude light but facilitate air exchange, at room temperature. Control media (PBS) was kept at room temperature in light. Observations were made in similar manner up to seven days. Study of the effects of different media: To find suitable media for the cultivation of eggs of Haemonchus contortus, a counted number of eggs was cultured in PBS, normal saline and tap water and incubated and observed as previously described. For the preparation of nutrient media, feces or soil were added to the PBS. A counted number of eggs was suspended in each culture medium and incubated at room temperature up to six days. The percentage of hatching eggs was calculated every 24 hours. Statistical analysis: Student t-test was used to determine the significance of differences between variables (Steel and Torrie, 1980).

Source of Information: Bangl. vet. 2013. Vol. 30, No. 1, 1 – 9

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Temperature, humidity, pH and light were important factors for in vitro cultivation of eggs of Haemonchus contortus. These factors influenced the development and hatching of the eggs. Media containing nutrients (PBS + faeces) improved the development and hatching of eggs.

Knowledge Management: Journal