Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Comparative Efficacy of Neem (azadirachta Indica) and Metformin Hydrochloride (comet®) in Streptozotocin Induced Diabetes Melitus in Rats

A. R. Das
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. Mostofa
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. E. Hoque
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

S. Das
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

A. K. Sarkar
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

The present study was investigated the comparative efficacy of extract of Neem (Azadirachta indica) and Metformin hydrochloride (Comet®) in respect of their hypoglycemic activity in Streptozotocin (STZ)-induced diabetic rats. Extract of Neem was administered @ 500 mg/kg body-weight (bwt) and 250 mg/kg bwt. Metformin hydrochloride @ 500 mg/kg bwt and 250 mg/kg daily orally for 21 days. Changes in the blood glucose level and body weight were measured and the results were compared statistically using Anova test. The extract of Neem leaves and Metformin hydrochloride showed significant (p<0.01) hypoglycemic effect and neem extracts at 250 mg/kg bwt showed more potent effects. The Neem extracts significantly (p<0.01) increased body weight @ 250 mg/kg. From this experiment it was observed that extracts of Neem leaves had hypoglycemic and body weight gain effect. For definite conclusion, details study is needed.

Keywords: Diabetes mellitus hypoglycemia, Blood glucose, Azadirachta Indica, Streptozotocin

Location: Mymensingh

Start Date: 01-01-2009

End Date: 31-05-2009

Program Area: Animal Health and Management

Commodity: Rats

Objectives:

This study has been performed to investigate the comparative hypoglycemic activity and body weight changes of aqueous extract of neem leaves and Metformin in streptozotocin induced diabetic rats.

Methodology:

This research work was conducted at animal Laboratory in the Department of Pharmacology, Bangladesh Agricultural University (BAU), Mymensingh from January/2009 to May/2009. Sixty long Evan’s strain (Ratus norvegicus), aged of 6 weeks and weighing between 180 to 190 gms were collected from International Center of Diarrhea Disease Research, Bangladesh (ICDDR,B). The rats were allocated into six groups, each containing 10 individuals. Each group of rats was housed at screen bottomed wire cages arranged in rows and kept in animal house. The animals were fed in pellet form at a recommended dose of 100gm/kg as advised by and purchased from (ICDDRB). Drinking water was supplied ad libitum. All the groups of rats were kept for 18 days for acclimatization. The rats were given water ad libitum, their body weights and blood glucose levels were measured and recorded after acclimatization at the time when that of other groups were measured. Group A was kept as normal control, Group B, as diabetic control group and groups C, D, E and F were diabetic treatment groups.Citric acid-l-hydrate, Tri-sodium citrate dihydrate, Streptozotocin (sigma), Accu CHEK(R) active monitor, Accu-chek test strip and other relevant instruments. 2. Preparation & Administration of Streptozotocin Solution: Streptozotocin was dissolved in 0.1 M citrate buffer having pH 4.5 which was injected intraperitonealy to rat and maintained fasting condition for 18 hours to induce diabetic condition in rat at a dose of 65 mg STZ per kg of body weight Fresh neem leaves were collected, grinded, 10 gm neem was weighted and mixed with 100 ml distilled water and kept it in hot air oven over 24 hours to become 10 ml which was administered orally with the help of micropipette to the experimental rats at various doses. Administration of drugs: Aqueous extract of neem leaves and Comet® tablet were fed orally to the experimental rats with the help of a micropipette.A drop of blood was collected from the tail vein. At the same time the Accu CHEK(R) Active monitor was started with a single soft press. After the monitor showed the code number the strip was inserted into the monitor. A drop of the blood was poured on the test zone of the strip. The values were expressed in m mol/L. 5. Determination of body weight by balance. Statistical analysis was made by using Anova test.

Source of Information: Bangl. J. Vet. Med. (2010). 8(1): 75 – 80

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In this research work, the continuous treatment with aqueous extract of Neem (Azadirachta indica) and Metforminhydrochloride produced a significant reduction (p<0.01) of the blood glucose level and body weight also significantly increased (p<0.01) in STZ-induced diabetic rats. Among two doses of aqueous extract of Neem, 250 mg/kg bwt was more potent. To establish Neem leaves and metformin hydrochloride (Comet®) as an antidiabetic agent scientifically, the underlying mechanism of hypoglycemic activity of Neem leaves and metformin hydrochloride (Comet®) need to be discovered. We did the work in short term basis as modern equipments were also not available. It is required to trial for longterm on other biochemicals and histopathological studies

Knowledge Management: Journal