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Study on Buck Evaluation Based on Semen Quality and Fertility

F Sultana
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

SS Husain
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

A Khatun
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

AS Apu
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

MAMY Khandoker
Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted to evaluate the performance of buck on the basis of semen quality and fertility. Data on 162 ejaculates from five adult Black Bengal bucks (B₁, B₂, B₃, B₄ and B₅) were recorded. Individual buck effect was found to be significant on semen volume (p<0.05), three stages of sperm motility (p<0.01) and percentage of live sperm (p<0.01) but insignificant (p>0.05) on sperm concentration and percentage of normal sperm. The results of semen volume per ejaculate, sperm concentration, initial sperm motility, on dilution sperm motility, post-thawing sperm motility, percentage of live sperm and percentage of normal sperm were ranged from 0.58±0.17 to 1.04±1.1 ml, 2827±0.76 to 3132±0.88 million/ml, 77.07±1.06 to 81.47±1.84%, 61.71±1.03 to 70.30±1.54%, 48.15±1.99 to 55.88±2.97%, 87.58±0.96 to 92.95±0.74% and 87.17±2.40 to 91.85±1.38%, respectively. The significantly (p<0.05) highest volume of semen, three stages of sperm motility and percentage of live sperm was found in buck B5 and lowest in buck B1. The significantly (p<0.05) highest non-return rate was found in buck B5 (87.31±7.99%) and lowest in buck B1 (63.41±672%). There was no significant (p>0.05) seasonal variation was observed on semen characteristics except on dilution sperm motility and post-thawing sperm motility which differed significantly (p<0.05). The season had no significant (p>0.05) effect on 25 day non-return rate of bucks. The results of the present study revealed that evaluation of breeding bucks based on semen quality is important and provides the guideline to buck evaluation for reproductive performance.

Keywords: Buck, Semen quality, Non-return rate

Location: Artificial Insemination Centre under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh

Start Date: 00-01-2008

End Date: 31-12-2009

Program Area: Animal Health and Management

Commodity: Goat

Objectives:

1. To evaluate the semen quality of Black Bengal bucks and also to investigate the effect of season on semen quality and fertility in respect of non-return rate (NRR).

Methodology:

The experiment was carried out at the Artificial Insemination Centre under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh during the period from 2008 to 2009. Five adult Black Bengal bucks (aged between 18 to 22 months) were taken for this experiment and were numbered as B₁, B₂, B₃, Semen was collected by artificial vagina (AV) method twice a week within 8.30 am from each buck. Semen samples were collected in a graduated transparent collection tube covered by an insulating jacket and kept in a water bath (37°C) immediately after collection for further evaluation. Evaluation of fresh semen was done. The volume of ejaculates (ml) was recorded from the graduated collection vials. The color and opacity of the semen and presence of any foreign body, such as pus were checked. Motility of raw semen or mass activity (%) was examined by examining a drop of raw undiluted semen on a pre-warmed (37°C) slide under a light microscope at 4x magnification. The concentration of spermatozoa in semen sample (million/ml) was estimated by haemocytometer method. Eosin-nigrosin stain was used for counting live spermatozoa whereas Rose Bengal stain was used for counting normal spermatozoa in semen. Semen with motility of more than 60% was diluted with freezing extenders egg yolk citrate diluter. Semen was diluted on the basis of volume and sperm concentration per ejaculate to obtain a final concentration of 100 million spermatozoa per insemination dose. The motility (%) of the diluted semen was observed and recorded. Semen was then packed manually into 0.5 ml straw and the laboratory ends of the straws were sealed with polyvinyl chloride (PVC) powder. The straws were placed in the refrigerator at 4-5°C for 3 hours. The motility of the equilibrated sperm was checked and only samples with more than 60-70% were used for freezing. After equilibration the straws were placed horizontally on a rack and transferred to the freezer to be frozen in vapor 15-20 cm above the liquid nitrogen (LN.). After 30 minutes, the frozen straws were transferred into the canister within the liquid nitrogen containers at -196°C until use for AI. After 24 hours, straws were retrieved from the LN containers using forceps and thawed in water bath at 37°C for 12 seconds and post-thaw motility was recorded. Artificial insemination with frozen semen was performed in BAU AI centers. The number of does inseminated with frozen semen by buck B₁, B₂, B₃, B₄ and B₅ were 49, 50, 50, 54 and 42, respectively. Non-return rate (NRR) was estimated. To evaluate the seasonal variations on semen quality and fertility, the experimental period was divided into 3 seasons, i.e. summer (March to June), rainy (July to October) and winter (November to February). The data generated were analyzed for ANOVA using a completely Randomized Design with the help of Statistical Analysis System Computer Package and DMRT (Duncan’s Multiple Range Test) was performed to identify significant differences among the mean values with the help of SAS, 1998.

Source of Information: Bang. J. Anim. Sci. 2013. 42 (2): 101-108

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the result it may be concluded that evaluation of breeding bucks based on semen quality is important and provides the guideline to buck evaluation for reproductive performance.

Knowledge Management: Journal