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Research Detail

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M. A. H. BHUIYAN
Principal Scientific Officer
Soil Science Division, Bangladesh Agricultural Research Institute (BARI), Gazipur-1701

D. KHANAM
Chief Scientific Officer (Ex.)
BARI, Gazipur-1701

M. T. RAHMAN
Senior Scientific Officer
Regional Agricultural Research Station, RARS, Ishurdi, Pabna.

M. H. R. SHEIKH
Principal Scientific Officer
RARS, Ishurdi, Pabna

M. M. H. BHUIYAN
Scientific Officer
RARS, Hathazari, Chittagong, Bangladesh.

Field experiments were carried out at Regional Agricultural Research Station (RARS), Ishurdi, Pabna during the rabi season of 2005-06 and 2006-07 to find out the effectiveness of Rhizobium strains for achieving higher yield of lentil, the variety BARI Masur–4. There were six treatments that were Rhizobium strains- BARI RLc-104, BARI RLc-105, BARI RLc-106, BARI RLc–107, mixed culture of the four strains, and control (no Rhizobium). The experiment was designed in randomized complete block having 4 replications of each treatment.The rhizobial inocula were peat based and used @ 1.5 kg/ha. A basal dose of P @ 22 kg/ha, K @ 42 kg/ha, S @ 20 kg/ha, and @ 5 kg Zn/ha was used for all treatments. The highest nodule number (11.7/plant in 2006 and 10.3/plant in 2007) and dry nodule weight (10.53 mg/plant in 2006 and 9.58 mg/plant in 2007) were found with mixed culture. Mixed culture produced the highest seed yield in 2006 (1.36 t/ha, 37.4% higher over uninoculated control) and in 2007, BARI RLc-104 recorded the highest seed yield (1.23 t/ha, 60.3% higher over uninoculated control). However, all the strains including mixed culture had similar seed yields over the years.

  Rhizobium; Lentil; Nodulation; Yield
  Regional Agricultural Research Station (RARS), Bangladesh Agricultural Research Institute (BARI), Ishurdi, Pabna
  00-00-2005
  00-00-2007
  Crop-Soil-Water Management
  Performance

To find out the effectiveness of some Rhizobium strain/(s) on lentil at RARS, Ishurdi, Pabna of Bangladesh.

Field experiments were carried out at Regional Agricultural Research Station (RARS), Bangladesh Agricultural Research Institute (BARI), Ishurdi, Pabna under Calcareous Dark Grey Floodplain Soils and in High Ganges River Floodplain (Agro-ecological Zone 11) during the rabi season of 2005-06 and 2006-07 to find out the effectiveness of Rhizobium strains on lentil. The initial soil sample of the experimental field was collected and analyzed following standard procedures in the laboratory. The soil was above neutral (pH 7.50) having low organic matter (1.05%). The content of total-N, available phosphorus, and available sulphur was very low. The exchangeable potassium was medium, available zinc was optimum, and available boron was low. The initial rhizobial population of the soil was below 103 . The experiment was laid out in randomized complete block design having four replications with six treatments. The unit plot size was 3 m × 4 m. The variety BARI Masur–4 of lentil and peat based rhizobial inocula BARI RLc-104, BARI RLc-105, BARI RLc-106, and BARI RLc-107 were used for the experiment. There were six treatments: T1: Rhizobium strains BARI RLc-104, T2: BARI RLc-105, T3: BARI RLc-106, T4: BARI RLc–107, T5: mixed culture of the four strains under study and T6: control (Without rhizobial inoculant). The studied peat based rhizobial inocula containing 108 cells/g inoculum were used @ 1.5 kg/ha. Chemical fertilizers i.e., P, K, S, and Zn were applied in all treatments as basal dose @ 22 kg P/ha from triple superphosphate, 42 kg K/ha from muriate of potash, 20 kg S/ha from gypsum and 5 kg Zn/ha from zinc oxide. The crop was sown on 23 November 2005 and 10 November 2006 with a spacing of 30 cm x 10 cm. During the course of the experiment, growth and development of plants in the field were carefully observed. Ten randomly selected plants along with roots were collected at 50% flowering stage from each unit plot and dry weight of roots, shoots, and nodules including nodule numbers were recorded. The plants were harvested on 02 March 2006 and 28 February 2007. Data on plant height, 1000-seed weight, stover yield, and seed yield were also taken plot-wise, pods/ plant and seeds/pod were taken from 10 randomly selected plants. Yield data were expressed on hectare basis. All data were analyzed statistically.

  Bangladesh J. Agril. Res. 39(1): 105-112, March 2014
  
Funding Source:
  

Mixed culture of lentil rhizobia showed better performance in nodulation. Seed yields were statistically non-significant between single and mixed culture treatments. Nitrogen content and uptake were higher in inoculated plants. Due to lower native rhizobial numbers and soil N characters, mixed culture inoculation approach might have a positive effect on lentil cultivation in Calcareous Dark Grey Floodplain Soil of Bangladesh. From two years’ results, it could be recommended that mixed culture of different Rhizobium strains or BARI RLc-104 was suitable for obtaining higher seed yield of lentil at western region of Bangladesh.

  Journal
  


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