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Comparative Efficacy of Korolla (momordica Charantia) Extract and Ivermec® Pour on with Their Effects on Certain Blood Parameters and Body Weight Gain in Indigenous Chicken Infected with Ascaridia Galli

H. M. Shahadat
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. Mostofa
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. A. A. Mamun
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. E. Hoque
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. A. Awal
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

Comparative anthelmintics efficacy of whole korolla fruit (Momordica charantia) extract and ivermec® pour on was evaluated in vitro and in vivo on adult Ascaridia galli of indigenous chicken. The total trial chickens (60) were divided equally into 3 groups; group A as control, group B treated with ivermec® pour on @ 500 μg/kg bwt by dropper through skin absorption for single dose and group C treated with 3% aqueous extract of korolla. Freshly prepared aqueous extract of the korolla fruit was performed as wormicidal properties against adult A. galli on in vitro and in vivo study. 3% aqueous extract of korolla fruit was treated as higher efficacy against A. galli. The live body weight was increased in chicken after treatment in group B and C respectively but in control group body weight was slowly decreased. TEC (million/cu mm), Hb (gm %) and PCV (%30 minutes) were increased significantly in chickens of treated groups whereas ESR was increased in control groups.

Keywords: Anthelmintics efficacy, Korolla, Lvermec® pour on, Ascaridia galli, Indigenous chicken, Haematological parameters

Location: Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Start Date: 00-02-2008

End Date: 00-05-2008

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

To evaluate comparative efficacy of korolla fruit (Momordica charantia) extract and ivermec® pour on against Ascaridia galli and also evaluate their effects on certain blood parameters and body weight gain in indigenous chickens.

Methodology:

The experiment was conducted at the Department of Pharmacology, Bangladesh Agricultural University, Mymensingh, during the period from February to May 2008. For In vivo Anthelmintic trial, a total of 60 indigenous chickens infected with Ascaridia galli were randomly selected from the local Market to study the comparative efficacy of korolla (Momordica charantia) extract and ivermec® pour on with their effects on certain blood parameters and body weight gain in indigenous chicken infected with Ascaridia galli. These chickens were between 45 to 60 days old and were selected on the basis of fecal examination. The body weights of assigned chickens were taken with digital weight machine and the results were recorded. During acclimatization the chicken were supplied with recommended feed and water. All the 60 fowls were randomly divided into 3 groups (A, B and C), each group containing 20 chickens and 4 sampling were performed on day 0 (just previously given treatment), 7, 14 and 21 to count number of parasites for assessing the efficacy of modern anthelmintic, Ivomec® (Ivermectin) pour on and korolla extract against ascariasis in chicken. Fowls of group ‘A’ were kept as control and not treated. Fowls of group ‘B’ were treated with ivermectin @ 500 μg/kg bwt by dropper through skin absorption for single dose. Fowls of group ‘C’ were treated with 3% aqueous extract of korolla. For in vitro trial, adult Ascaridia galli were collected from the intestine of chickens slaughtered in local markets using a standard method and placed in a petridish containing normal saline and was kept in incubator at 38⁰C until required for experiment. 8 adult parasites were placed on petridish with 3% aqueous extract of korolla fruit and another 8 adult parasites were placed on petridish with normal saline. During the study, the efficacy of korolla fruit extract was made in-terms of mortality of the treated parasite. Mean live weight gain of each group of fowls on day 0, 7, 14 and 21 were recorded. Fowls under trial and control groups were weighed with Electric weighing machine. The weight of each fowl was taken before feeding in the morning and afternoon. The average of these two weights was calculated and recorded. Haematological examinations were carried out in all the groups of chickens. About 0.5 ml of venous blood was collected from the wing vein at pre-feeding (0 day), day 7, 14 and 21 at 7 days interval and mixed with anticoagulant (Na-citrate 1%) at 1:5 ratio. The collected samples were brought to the laboratory and the haematological parameters (TEC, Hb content, PCV and ESR) were determined as per method described.

Source of Information: Bangl. J. Vet. Med. (2008). 6 (2): 153–158

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From this study, it is concluded that the 3% aqueous extract of korolla fruit (whole) having significant anthelmintic activity on adult A. galli by giving in vitro and in vivo trial. In this case, only the 3% concentration and adult parasites were used. So, study should be conducted to determine the anthelmintic effects of various concentration of this plant material against the various developmental stages (e.g. egg, embryonated egg) of A. galli. Further, in vivo trial should be given in large scale field condition to determine the toxic effects of this plant material, if any. By selecting accurate dose of aqueous extract of korolla fruit through in vitro and in vivo study, the farmers of Bangladesh can cheaply control the adult Ascaridia galli infection in indigenous chickens.

Knowledge Management: Journal