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In Vitro Microtuberization of Potato (solanum Tuberosum L.) Cultivar Through Sucrose and Growth Regulator

K. Momena
Advanced Seed Research and Biotech Center, ACI Limited, Dhaka

L. Rahman
Advanced Seed Research and Biotech Center, ACI Limited, Dhaka

Saiful Islam
University of Development Alternative, Dhaka, Bangladesh

H. Mehraj
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka

A.F.M. Jamal Uddin
Associate Professor
Dept. of Horticulture, Sher-e-Bangla Agricultural University, Dhaka

R. Adeeba
Advanced Seed Research and Biotech Center, ACI Limited, Dhaka

Abstract/ Executive Summary:

Microtubers which are produced in tissue culture have benefits for disease free potato production. As growth regulators and sucrose concentration affect the initiation and growth of microtuber, therefore, an experiment was conducted to discover the suitable growth supplement to produce disease free potato microtuber. Four potato cultivars viz. Granula, Daimond, Sarpoaxona and Lal Pakri1 with three different treatments viz. T₁ = 8% Sucrose, T₂ = 6% Sucrose + 4 mg/L Kinetin, T₃ = 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP were used for micro tuberization as supplement of Murashige and Skoog (MS) medium. Among tested cultivars, Diamond performed better compare to other three potato cultivars with treatment combination T₃. Maximum number of culture showing microtuber (56.0), explant formed microtuber (93.3%), number of microtuber (2.7), average diameter (4.42 mm) and average weight (97.61 mg) were found from Diamond cultivar in 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP.

Keywords: Potato cultivars, Microtuberization, Sucrose, Kinetin and BAP

Location: Advanced Seed Research and Biotech Center, ACI Limited, Dhaka.

Start Date: 00-01-2014

End Date: 00-04-2014

Program Area: Variety and Species

Commodity: Potato

Objectives:

To produce disease free potato microtuber which could be used for commercial microtuberization.

Methodology:

Healthy and disease-free explants (Sprouts) of potato (Solanum tuberosum L.) were used for this experiment which was carried out from January to April, 2014 in Advanced Seed Research and Biotech Center, ACI Limited, Dhaka; and supervised from Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Four different potato cultivars viz. Granula, Daimond, Surfo-exona and Lalpakri1 were used for the experiment. Potato tubers were washed several times with detergent followed by several times rinsing with distilled water, then the tubers were soaked in GA3 50 mg/l concentration for at least three hours, after that the tubers were dried well and kept in dark room with humidity till the sprouting started. One-week old healthy sprouts were taken and washed with a few drop of detergent and then washed in running tap water for at least 30 minutes. Then the sprouts were taken to the laminar airflow for further sterilization to avoid any type of pathogenic contamination. At first the sprouts were washed with 70% ethanol for only 30 seconds then they were rinsed three times with autoclaved double distill water 15 minutes, then dipped in 0.1% HgCl₂ for 7 min and again five times rinsed with autoclaved double distill water for 25 minutes. Then the sprouts were put on the MS basal medium supplemented with 1.5mg/l BAP and 0.5mg/l GA₃ for shoot initiation. After 4 weeks, buds sprouted into full plantlet having 7–8 nodes. Single node cuttings were excised and inoculated on MS basal medium supplemented with 4mg/l BAP for further in vitro multiplication. The process was continued until complete plantlets were obtained in sufficient numbers. Four-weeks old plantlets were excised into shoot apices and nodal cuttings, which were used as explants. One apical explant was inoculated on medium in each culture tube and 4-5 multi nodal explants were inoculated in jars. 10 test tubes and 10 jars were prepared in such a way. Three different treatments viz. T₁ = 8% Sucrose, T₂ = 6% Sucrose + 4 mg/L Kinetin, T₃ = 8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP were used for micro tuberization. So for each concentration 10 replicates were prepared. Data was taken on number of microtuber formation, percentage of microtuber formation, days to microtuber induction, average diameter and average weight. After 10 weeks of incubation, microtubers were harvested in pre-weighed and sterilized polypropylene bags aseptically and their diameter and fresh weight was recorded.

Source of Information: Journal of Bioscience and Agriculture Research Vol. 02 (02): 76-82, 2014, Online ISSN 2312-7945

Article Link (Online Journal): http://www.journalbinet.com/uploads/2/1/0/0/21005390/in_vitro_microtuberization_of_potato_solanum_tuberosum_l._cultivar_through_sucrose_and_growth_regulator.pdf

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Four different potato cultivars and three treatment combination with sucrose and growth regulators that were used in this experiment revealed significant source of variation for potato microtuberization. The cultivar that exhibited best microtuberization was Diamond; and treatment T₃ (8% Sucrose + 4 mg/L Kinetin + 1 mg/L BAP) was most potential for potato microtuberization compare to other treatments of this experiment.

Knowledge Management: Journal