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Comparison of Two Hormone Preparations on the Reproductive Performance of Air Breathing Catfish Clarias Batrachus (lin.)

S. U. Mahmood
Department of Zoology, University of Dhaka, Dhaka 1000, Bangladesh

K. Ahmed
Bangladesh Agricultural Research Council, Dhaka

M.S. Ali
Department of Zoology, University of Dhaka, Dhaka 1000, Bangladesh

M.E. Hoque
Rangamati Govt. College, Rangamati

Abstract/ Executive Summary:

Two hormone preparations viz. Human ?Chorionic Gonadotropin (HCG) and pituitary gland (PG) suspension were compared for their comparative efficacy on the breeding performance of a air breathing catfish Clarias batrachus. It was found that HCG induced fish gave better ovulation response than PG. Both fertilization and hatching of eggs were significantly (pHCG treated fish than PG. On all consideration, HCG was found more suitable for induced breeding of C. batrachus over PG.

Keywords: HCG, PG, Clarias batrachus, Breeding

Location: Dohar, Dhaka, Bangladesh.

Start Date: 00-00-2001

End Date: 00-00-2001

Program Area: Variety and Species

Commodity: Cat fish

Objectives:

To study two hormone preparations HCG and PG to compare their comparative efficacy on the breeding performance of C. batrachus.

Methodology:

The study was carried out in a commercial fish breeding farm, Madina Fisheries and Hatchery Complex located at Dohar, Dhaka, Bangladesh. Thirty matured female catfish Clarias batrachus were collected from hatchery's own brood ponds. Twenty fish were selected for the experiment. The surplus 10 fish was kept as a backup in case of mortalities and escapes. Furthermore, 10 males were used from the fish farm's own brood stock to provide milt for fertilization. After proper acclimatization, the fishes were divided into two groups having 10 females in each groups and designated as T₁ and T₂. The two hormone preparations were collected from local market. The dry PG (pituitary gland of carp) in vial is available in market and generally used for carp breeding in Bangladesh. The HCG was produced by a commercial pharmaceutical company (Sumach: Infar, India) and HCG solution was prepared according to the prescriptions of brochure supplied by the manufacturing company. Dry HCG powder (30 i.u./mg) was dissolved· in distilled water. After homogenizing, the solution was centrifuged for 5 minutes at 4,000 rpm. The clear upper fluid was decanted in a sterile 30 ml flask and used immediately. HCG was diluted in such a way that 1 ml solution corresponds to 1000 i.u. The PG solution was prepared by homogenizing the dry PG in a tissue homogenizer and centrifuged in a centrifuge machine at 4,000 rpm. The upper clear fluid is used for injection purpose. Ten fully matured male brood fish were used for sperm collection. The fish were dissected and the sperm collected from the testes by squeezing out the milt. On mid June 2000, all experimental female fish were arrangedinto two groups of 10 females with a mean body weight of 119 ± 5 g and 118 ± 5 g, respectively. Each experimental female was injected with one of the two hormone preparations. The suspension was injected into dorsal musculature just below the dorsal fin. One group (T₁) was injected with 400 i.u. HCG/100 g body weight and the other group (T₂) was injected with 15 mg PG/ 100 g body weight. In each case total dose of hormone was divided into two injections and given at 6 hour interval. After injection, the females were individually housed in 200-1 trays. A latency period of approximately 18 hours was allowed after which the fish were stripped for egg. The eggs from each individual female was collected in plastic bowl and fertilized with milt. Approximately 100 eggs from each female was incubated in 20-1 plastic bowl fitted with inlet and outlet for constant water supply. In all 20 plastic bowls was used for egg incubation. Water temperature was maintained at 27 ± 1 °C. After about 26 hours of incubation all egg samples were thoroughly checked, dead eggs were counted per plastic bowl and removed, leaving the hatched larvae behind. The percent ovulation was calculated by the formula: Percent ovulation= No. offish ovulated :x 100 I Total no. of fish injected. The fertilization rate was calculated by following formula: Percent fertilization = No. of fertilized eggs x 100 I Total no. of eggs (fertilized + unfertilized). The hatching percentage was calculated after hatching. The hatching percentage was calculated as [(Number's of eggs in sample - Dead eggs) I Number of eggs in sample x 100. Data were analyzed by using the computer based SPSS program.

Source of Information: Bangladesh J. Fish. Res., 7(l), 2003: 21-26

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The use of HCG in induced breeding of C. batrachus could give better yield if proper induced breeding technology in the farmer level can be disseminated.

Knowledge Management: Journal