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Selection of Salt Tolerant Cell Lines from Embryo Derived Calli of Indica Rice (oryza Sativa L)

M. F. Hossain
MS Student
Department of Genetics and plant Breeding, Bangladesh Agricultural University, Mymensingh-2202

M.S. Alam
Professor
Department of Genetics and plant Breeding, Bangladesh Agricultural University, Mymensingh-2202

M A Samad
CSO
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202

Abstract/ Executive Summary:

Attempts were taken for callus induction followed by selection of salt tolerant cell lines in four varieties of indica rice. High frequency of callusing from mature embryo was observed in Binnatoa (70%) followed by Pokkali (60%), BR16 (52%) and BR 26 (34%) when cultured in MS medium. The calli were then transferred to MS regeneration medium supplemented with different doses of NaCI (0.0.1,0.3 and 0.5%) to select salt tolerant cell lines. Both callus growth and plant regenerations were decreased with the increased level of NaCI in the media. The highest frequency of plant regeneration (33%) was observed in the variety Pokkali with 0.1% NaCI followed by Binnatoa (30%), BR 26 (14%) and BR 16 (12%), respectively. Pokkali and Binnatoa exhibited 12% and 7% regeneration respectively at 0.5% NaCI whereas no regeneration was observed in BR16 and BR 26 at the same concentration. Regenerants of salt tolerant varieties Pokkali and Binnatoa stressed to 0.5% NaCI when cultured in vivo, exhibited increased survival rate compared to control.

Keywords: Rice, Mature embryo, Regenerations, Salt tolerant

Location: Tissue Culture lab., Plant Breeding Division, BINA, Mymensingh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Rice

Objectives:

To investigate the effect of NaCl on callus growth and plant regeneration and screening for salt tolerant cell lines in four indigenous indica rice varieties.

Methodology:

Rice (Oryza sativa L) cv. Binnatoa, Pokkali, BR16 and BR 26 (Collected from Bangladesh Institute of Nuclear Agriculture, Mymensingh) were used in this study. Mature and healthy seeds of these varieties were dehusked and surface sterilized in 0.1% HgCI₂ for 20 minutes followed by 4-5 rinsed in autoclaved distilled water to remove traces of HgCI₂. Sterilized seeds were then cultured in MS (Murashige and Skoog 1962) medium with 1.5 mg/ 12, 4-D, 0.25mg/ 1 BAP; 30 g/1 sucrose and 7% agar to initiate callus. After 2 to 3 weeks of inoculation, calli were developed. These calli were subjected to regeneration medium (MS with 0.5 mg/ 1NAA. 10 mg/1 Kn, 70 g/1 sucrose and 7% agar) supplemented with 0, 0.1, 0.3 and 0.5% NaCI. Proliferation of callus as well as frequency of regeneration were studied in an ideal growth room. After 4 weeks of growth in the media, regenerated plantlets of Binnatoa and Pokkali (0.5% NaCI level) were washed to make free of agar in running tap water and transferred to the earthen pots containing garden soil and covered with perforated polythene-bags for hardening and establishment. After 2 to 3 weeks of establishment, the regenerated plants along with control were treated with nutrient Hogland solution containing 0.5% NaCI for screening salt tolerant variety under in vivo condition.

Source of Information: Bangladesh J. PI. Breed. Genet, 13(2): 01-05, 2000

Article Link (Online Journal):

Funding Source:

1. Asian Development Bank

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Regenerants of salt tolerant varieties Pokkali and Binnatoa stressed to 0.5% NaCI when cultured in vivo, exhibited increased survival rate compared to control.

Knowledge Management: Journal