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Biological Screening of Curcuma Longa L. for Insecticidal and Repellent Potentials Against Tribolium Castaneum (herbst) Adults

Y. Abida
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh

F. Tabassum
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh

S. Zaman
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh

.B. Chhabi
Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh

N. Islam
Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

The rhizome and aerial part extract offered dose mortality action against T. castaneum adults were found promising and the results have been presented. The LD₅₀ values for rhizome extract were 0.337 and 0.201 mg cm^-2 for 24 and 48 h of exposure respectively while aerial part extract were 0.695 and 0.639 mg cm^-2. The rhizome extract was found stronger than the aerial part extract.

Keywords: Biological screening, Curcuma longa L., Potentials, Tribolium castaneum (Herbst)

Location: Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insects

Objectives:

To evaluate the efficacy of the test plant as a possible source of potential secondary metabolites to be used as environment friendly pest control agents.

Methodology:

Fresh aerial part and rhizomes of C. longa were collected from the adjacent areas of Rajshahi University Campus. The collected materials were dried under shade and powdered in a blender machine avoiding excess heat during blend. The ground materials were extracted with chloroform and left to evaporate for experimental use. The red flour beetle, T. castaneum were collected from the stock cultures maintained in the Crop Protection and Toxicology Laboratory, University of Rajshahi and reared in glass beakers (500 ml) with standard food medium (wheat flour: dry dust yeast. 19:1) in an incubator at 30°C ± 0. 5°C without light and humidity control for continuous supply of adults for the experiments. For dose-mortality through surface film assay general concentrations for C. longa rhizome and aerial part extracts were selected as 50mg/ml and 90 mg/ml as the stock doses. The rhizome extract offered other successive doses 1.769, 0.885, 0.442, 0.221 and 0.111 mg cm-2 and the aerial part extract offered 3.185, 1592, 0.796, 0.398 and 0.199 mg cm^-2 through serial dilution. Then 1 ml of each of the doses poured onto each of the Petri dishes (6 cm diam.) and let them dried out before releasing 10 beetles (of 3-5 days old) in each. Mortality of the beetles was counted after 24 and 48 h of exposure. The mortality (%) was corrected and the statistical analyses were done to find the LD₂₀ values.

Source of Information: Univ. j. zool. Rajshahi. Univ. Vol. 28, 2010 pp. 69-71; ISSN 1023-6104

Article Link (Online Journal): http://journals.sfu.ca/bd/index.php/UJZRU

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The petroleum ether extract of the leaves and rhizome exhibited toxicity towards the mosquito species: Culex pipiens, C. quinquefasciatus, Aedes aegypti, Anopheles stephensi, larvae.

Knowledge Management: Journal