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Screening of Pumpkin Genotypes Against Cucumber Mosaic Virus (cmv) Through Artificial Inoculation

M. S. Rahman
Plant Pathology Division, BARI

A.U. Ahmed
Plant Pathology Division, BARI

M.N. Islam
Plant Pathology Division, BARI

K.M. Alam
Plant Pathology Division, BARI

Abstract/ Executive Summary:

The experiment was conducted in net house of Plant Pathology Division, BARI Gazipur to find out CMV resistant/tolerant pumpkin genotype or line through artificial inoculation. Forty germplasm were collected from PGRC, BARI and evaluated against CMV. Among the germplasm eleven accessions (BD4357, BD4359, BD4360, BD4364, BD4370, BD4378, BD4382, BD4391, BD9872, BD9877 and BD10297) were found highly susceptible, fifteen accessions (BD4349, BD4350, BD4353, BD4354, BD4368, BD4372, BD4381, BD4383, BD4395, BD9871, BD9875, BD9879, BD9880, BD10295 and BD10295) were recorded as susceptible, six accessions (BD4348, BD4358, BD4371, BD4380, BD4393, BD4397 and BD9889) were graded as moderately susceptible, four accessions (BD4351, BD4352, BD4363 and BD9870) were graded as moderately resistant and three accessions (BD4355, BD4361 and BD10303 were found to be resistant against CMV under artificial inoculation.

Keywords: Pumpkin genotype, Cucumber mosaic virus, Germplasm

Location: Plant Pathology Division, BARI Gazipur

Start Date: 00-00-2014

End Date: 00-00-2015

Program Area: Pest Management

Commodity: Diseases, Sweet gourd

Objectives:

The present piece of research was undertaken to find out CMV resistant/tolerant pumpkin genotype / line.

Methodology:

The experiment was conducted in the net house of Plant Pathology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur during 2014-15. Seeds of pumpkin genotypes were collected from Plant Genetic Resource Centre, BARI. Seedlings of collected pumpkin genotypes were raised in insect proof net house in polythene bags containing sterilized soil. The seedlings were inoculated with CMV isolate in cotyledon leaves. Mechanical sap transmission technique was followed, described by Hill 1984. Potassium Phosphate (0.01M) buffer pH 7.1 was used for sap preparation. The leaves of the test plants dusted with sterilized 600 mesh carborundum powder. The sap was rubbed with finger onto the carborundum powder dusted leaves of the test plants. After rubbing with sap the inoculated leaves were carefully washed with sterilized water using wash bottle. The plants were checked everyday to detect the appearance of symptoms. Disease scoring was recorded 0-5 scoring scale described by Masud et al.(2005) where 0=No visible symptom (HR), 1= up to 1 % leaf area infection (R), 2= >1 to 5 % leaf area infection (MR), 3= >5 to 25 % leaf area infection (MS) and 4=>25 to 50 % leaf area infection (S) and 5=> 50 % leaf area infection (HS). The experiment was carried out in Completely Randomized design (CRD) having three replications with un-inoculated control. All data were analyzed statistically using R-software. Treatment mean were compared using DMRT.

Source of Information: Annual research Report, Plant Pathology Division, BARI--2014-15

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Pumpkin germplasm reactions against CMV were graded as resistant, moderately resistant, susceptible and highly susceptible. Among the forty accessions, eleven accessions namely BD4357, BD4359, BD4360, BD4364, BD4370, BD4378, BD4382, BD4391, BD9872, BD9877 and BD10297 were graded as highly susceptible where disease score ranged from >4 to 5. Fifteen accessions namely BD4349, BD4350, BD4353, BD4354, BD4368, BD4372, BD4381, BD4383, BD4395, BD9871, BD9875, BD9879, BD9880, BD10295 and BD10295 were graded as susceptible having disease score >3 to 4. Six accessions namely BD4348, BD4358, BD4371, BD4380, BD4393, BD4397 and BD9889 were graded as moderately susceptible where disease score from >2 to 3. Four accessions i.e. BD4351, BD4352, BD4363 and BD9870 were graded as moderately resistant and disease score was >1 to 2. Only three accessions namely BD4355, BD4361 and BD10303 were found to be resistant against CMV under artificial inoculation. Results of the present investigation prove that natural resistance exists in tested pumpkin germplasm against CMV. However, three accessions were found to be the best against CMV under artificial inoculation.

The accessions BD4355, BD4361 and BD10303 may possess resistant to CMV which may be selected as promising resistant lines. However, small number of symptom less plants was observed among the accessions after inoculation. So, further screening for resistant to CMV is necessary for final conclusion.

Knowledge Management: Report/Proceedings