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Multivariate Analysis in F4 Lines of Brassica Rapa L.

M H Khan
ORC, BARI

S R Bhuiyan
Dept. of Genetics & Plant Breeding, SAU, Dhaka

M H Rashid
ORC, BARI

M S Huda
Farm Division, BARI

I Ahmed
PGRC, BARI

Abstract/ Executive Summary:

A field experiment was conducted in the experimental field of Genetics and Plant Breeding Department, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh, during November 2008 to February 2009 to study genotypic variability and genetic diversity in 10 F4 lines obtained through intervarietal crosses along with 8 released varieties of Brassica rapa. Significant variation was observed among all the genotypes for all the characters studied. Multivariate analysis was performed to classify 18 genotypes. All the genotypes were grouped into four clusters. Principle Component Analysis, Principle Coordinate Analysis, Cluster Analysis, Canonical Variate Analysis gave similar results. Cluster IV was the largest cluster comprising of 7 genotypes and cluster II was the smallest cluster with 2 genotypes. Cluster II had the highest intra-cluster distance and Cluster I had the lowest intra cluster distance. Inter cluster distance was maximum between clusters II and III. The results revealed that genotypes chosen for hybridization from clusters with highest distances would give high heterotic F1 and broad spectrum of variability in segregating generations. The characters- no. of primary branches/plant, no. of secondary branches/plant and days to 50% flowering contributed maximum towards divergence among Brassica genotypes. Considering cluster distance, inter genotypic distance and other agronomic performance G2 and G14 from cluster I; G18 from cluster II; G1, G9 and G12 from cluster III and G16 and G17 from cluster IV may be considered to be better parents for future uses in hybridization program.

Keywords: Brassica rapa, Genotypic variability, Genetic diversity, F4 lines

Location: Experimental field of Genetics and Plant Breeding Department, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh

Start Date: 00-11-2008

End Date: 00-02-2009

Program Area: Variety and Species

Commodity: Mustard

Objectives:

The present research work has been undertaken to compare the yield potential and yield contributing characters of different promising F4 lines, to analyze the genetic variability of the genotypes in respect of different morphological characters and to screen out the suitable genotypes for future use.

Methodology:

A field experiment was conducted in the experimental field of Genetics and Plant Breeding Field Laboratory of Sher-e Bangla Agricultural University, Dhaka, Bangladesh during the period from November 2008 to March 2009 to study the genotypic variability of F4 lines in Brassica rapa. The soil of the experimental area was loamy belonging to the Madhupur Tract under AEZ 28.The soil of the experimental plots were clay loam, land was medium high with medium fertility level. Eighteen genotypes were used in the study. During the rabi season the rainfall generally is scant and temperature moderate with short day length. Meteorological data on rainfall, temperature, relative humidity from November 2007 to March 2008 were obtained from the Department of Meteorological centre, Dhaka-1207, Bangladesh. The experiment was conducted using ten F4 lines along with their five parental materials and three check variety. The fertilizers N, P, K, S and B in the form of Urea (250kg/ha), TSP (170 kg/ha), MoP (85 kg/ha), Gypsum (150 kg/ha) and Borax (1kg/ha), respectively were applied. The entire amount of TSP, MP, Gypsum, Zinc sulphate and borax was applied during the final preparation of land. Urea was applied in two equal installments at before sowing and flowering. Field lay out was done after final land preparation. The seeds of parents and F4 materials were laid out in a Randomized complete block design (RCBD) with three replications. The total area of the experiment was 25m × 5m = 125 m2. Each replication size was 25 m × 1.6 m. A distance of 1 m from block to block, 30 cm from row to row and 6 cm from plant to plant was maintained. Seeds were sown in lines in the experimental plots on 11th November, 2008 by hand uniformly. The seeds were placed at about 1.5 cm depth in the soil. After sowing the seeds were covered with soil carefully so that no clods were on the seeds. Seed germination started after 3 days of sowing on 14th November 2008. Treatment was distributed in the operations were accomplished for better growth and development of the Brassica seedlings. Malathion 57 EC insecticide was applied after one month of seeds sowing at 12 days interval for 3 times with 2 ml per liter water for protecting the crop from the attack of aphids and Rovral-50 WP was sprayed @ 20g/10L water first one at the time of siliquae setting of fruiting and second one after 15 days of 1st spraying to control Alternaria leaf spot. No other remarkable disease attack was observed. Harvesting was started from 5 February, 2009 depending upon the maturity of the plants. When 80% of the plants showed symptoms of maturity i.e.; straw color of siliquae, leaves, stem and desirable seed colour in the matured siliquae, the crop was assessed to attain maturity. Ten plants were selected at random from each plot in each replication. The sample plants were harvested by uprooting and then they were tagged properly. Data were recorded from these plants. For studying different genetic parameters and interrelationships ten characters were taken into consideration. Data were recorded on from the randomly selected ten plants. Mean data of each character was subjected to multivariate analysis viz, Principal Component Analysis (PCA), Principal Coordinate Analysis (PCO), Canonical Vector Analysis (CVA) and Cluster Analysis (CLA) using ‘Genstat’ released 13 programme by a IBM Computer following the method of multivariate analysis.

Source of Information: Eco-friendly Agril. J. 6(09): 199- 204, 2013 (September); ISSN 199-7957

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Considering the magnitude of genetic distance, contribution of character towards divergence, magnitude of cluster mean and agronomic performance the genotypes G2 for higher seed yield/plant and for highest 1000 seed wt., G8 for lower plant height, lower days to 50% flowering and G14 for lower days to maturity from cluster I; G18 for highest length of siliquae and lower days to maturity from cluster II; G1 for highest no. of primary branches/plant and for highest seed yield/plant; G3 for higher number of siliquae/plant and G9 for lower days to 50% flowering, G12 for highest no, siliquae/plant from cluster III; G16 for lowest days to maturity and G17 for lowest days to 50% flowering and plant height from cluster IV were found promising. Therefore considering group distance and other agronomic performances genotypes G8, G9, G12 and the inter genotypic crosses between G2 and G17; G3 and G17; G1 and G17; G2 and GI; G1 and G16 may be suggested for future hybridization program.

Knowledge Management: Journal