Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Different Explant and Concentration of Zeatin Riboside for in Vitro Regeneration of Potato

M S Huda
Farm Division, BARI Gazipur

M M Hossain
Dept. of Horticulture, BSMRAU, Gazipur

M Zakaria
Dept. of Horticulture, BSMRAU, Gazipur

M Z Haq
Seed Science and Technology Unit BSMRAU, Gazipur

A Hannan
Seed Technology Division BARI, Gazipur, Bangladesh

Abstract/ Executive Summary:

Keywords: Zeatin Riboside, In vitro regeneration, Potato

Location: Tissue Culture Laboratory of Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur

Start Date: 00-00-2012

End Date: 00-00-2012

Program Area: Variety and Species

Commodity: Potato

Objectives:

The present study was undertaken to investigate the direct in vitro regeneration performance of different explants of potato (Asterix), and optimize the concentration of Zeatin riboside (ZR) as a cytokinin in MS medium for efficient regeneration.

Methodology:

The experiment was conducted at the Tissue Culture Laboratory of Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh in 2012. The different explants i.e. i. Shoot ii. Leaf iii. Node and iv.Internode were used in MS media supplemented with 1.0 mg L^-l ZR to find out suitable explant for direct regeneration of potato. And to find out suitable concentration of ZR the nodal explants were placed in MS media supplemented with different (0.0 mg L^-l, 1.0 mg L^-l, 2.0 mg L^-l, 3.0 mg L^-l, 4.0 mg L^-l, 5.0 mg L^-l, and 6.0 mg L^-l) concentration of ZR. Preparation of explants - In vitro plantlets of 25-30 days old at solid medium was considered for the explants collection. For stem explants, only the first 5-6 nodes from the top of the plantlet excluding shoot apex of the each plantlet were excised. Internodes and node segments were excised to avoid the auxiliary buds and divided into 0.5- 1.0 cm long segments. For leaf explants, thick and healthy leaves from the upper nodes of the plants were used. The leaf tips and basal portions, including the petiole were discarded and the entire leaf was cut into 5 mm x 5 mm pieces. The leaf explants were placed upside down on the medium. For shoot explants, only 8-10 mm apex shoot were used where side leaves were discarded. All the explants were placed in test tubes and fuel paper were used and kept in a growth room at 25±2 °C. Only the first five internodes from the top of the plantlets were excised. Using alcohol, the laminar air flow chamber was thoroughly cleaned to maintain the aseptic condition. Under aseptic conditions, young and tender plantlets were taken out on a sterile glass plate using sterile forceps. The plantlets were not surface sterilized as those were already maintained under in vitro aseptic conditions. The roots of these plantlets were excised using a sterile scalpel and the leaves were removed. Preparation of media, inoculation of explants and incubation - To evaluate the performance of different types of explant for direct regeneration the composition of MS media supplemented with1.0 mg L^-lZR. And to find out the suitable concentration of ZR the media was supplemented with different concentration (0.0 mg L^-l, 1.0 mg L^-l, 2.0 mg L^-l, 3.0 mg L^-l, 4.0 mg L^-l, 5.0 mg L^-l, and 6.0 mg L^-l) of ZR. Fine agar powder of Loba, India brand was used. After PH adjustment 8 g L^-l agar was added to the solution to solidify the media. An amount of 10 ml media were dispensed into each test tube. The test tubes were autoclaved at l2l⁰C for 20 minutes at 1.2 kg/m² and thereafter stored at 25⁰C. After 2-3 days, the media was checked for any type of contamination and the explants were inoculated after performing several sequential steps. The explants were placed in each plate for each treatment horizontally in contact with MS medium supplemented with ZR. The treatment was replicated for three times under CRD design. Every ten test tubes were used for one replication. The test tubes were sealed, labeled carefully and were finally kept in the culture room under constant temperature (25⁰C) and light (16 hrs. light, 8hrs. dark), and the growth of the inoculated explants was monitored regularly. Only plantlets with well developed roots were used for multiplication in net house. Data on percent explants establishment (green internodes slices only) was recorded after two weeks and buds per explants after five weeks. The data were analyzed statistically and means were separated by Least Significance Difference method.

Source of Information: Eco-friendly Agril. J. 6(07): 128-130, 2013 (July); ISSN 1999-7957

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

From the above discussions, it may be concluded that for direct regeneration of potato (Asterix), the nodal explants is suitable for direct regeneration and 4 mg L^{- 1} to 5 mg L^-1Zeatin Riboside (ZR) supplemented with MS media showed the best performance regarding in vitro direct regeneration of potato from shoot explants.

Knowledge Management: Journal