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Haematobiochemical Pictures of Healthy Hubbard Classic Broiler Birds

M. O. Faruqe
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. R. Alam
Department of Physiology, Pharmacology and Biochemistry, Chittagong Government Veterinary College, Chittagong - 4202.

M. E. R. Bhuiyan
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

M. Myenuddin
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

To evaluate the certain haematobiochemical parameters in different ages of Hubbard Classic broiler birds, the study was performed at Kazi Farms Ltd., Gazipur in collaboration with the Department of Physiology, Bangladesh Agricultural University, Mymensingh for a period of one month (1^st to 30^th March 2002). On haematobiochemical examination, total erythrocyte count, haemoglobin content, packed cell volume, clotting time, glucose and uric acid concentration were gradually increased with advancement of age of birds. The values of TEC, Hb, PCV, clotting time, glucose and uric acid were recorded at day old birds as average 1.23 ± 0.02 million / cmm, 3.90 ± 0.09 g%, 19.60 ± 0.24 % , 37.20 ± 1.50 second, 179.20 ± 8.95 mg / dl and 3.72 ± 0.58 mg /dl respectively while the values at day 30 were 2.43 ± 0.07 million / cmm, 7.26 ± 0.16 g%, 28.20 ± 0.58%, 206.40 ± 10.17 second, 271.60 ± 8.56 mg / d. and 6.42 ± 0.65 mg /dl respectively which were found statistically significant (p < 0.01). On the other hand erythrocyte sedimentation rate, cholesterol and urea concentration were gradually decreased where significantly (p < 0.05) higher values (2.80 ± 0.20 mm in 1^st hour, 330.40 ± 38.52 mg /dl and 26.42 ± 2.06 mg /dl respectively) were recorded at day old chicks and lower values (1.80 ± 0.37 mm in 1^st hour, 104.20 ± 3.24 mg /dl and 17.00 ± 0.07 mg /dl respectively) were recorded at age of 30 days old of birds. The findings of the present work clearly indicate that age has got influential effect on the body haemopoietic system of the broiler birds as well as haematobiochemical values.

Keywords: Broiler, Haematological, Biochemical, Values

Location: Kazi Farms Ltd., Sreepur, Gazipur in collaboration with the Department of Physiology of Bangladesh Agricultural University (BAU), Mymensingh.

Start Date: 00-03-2002

End Date: 30-03-2002

Program Area: Animal Health and Management

Commodity: Poultry

Objectives:

To study the normal haematobiochemical parameters of broiler birds

Methodology:

The experiment was conducted at Kazi Farms Ltd., Sreepur, Gazipur in collaboration with the Department of Physiology of Bangladesh Agricultural University (BAU), Mymensingh for a period of 1 month from 1^st to 30^th March 2002. A total of 20, day-old Hubbard Classic broiler chickens were used to study the effect of age on the hacmatobiochernical parameters namely, total erythrocyte count (TEC), haemoglobin concentration (Hb), packed cell volume (PCV), erythrocyte sedimentation rate (ESR), clotting time, blood glucose, cholesterol, urea and uric acid. The chickens were reared throughout the entire period of study in the well ventilated and protected poultry shed of Kazi Farms Ltd., Gazipur with proper light, space, water supply, sanitation and litter. During the experimental period the birds were maintained with standard broiler starter and finisher feed formulated by Kazi Feed Ltd. as well as vitamin mineral premix supplementation. The routine vaccination was done properly. On the particular day of blood collection birds were brought to the physiology laboratory in a well ventilated bamboo boxes to avoid the danger of suffocation. Blood samples were collected aseptically with syringe and needle either from heart or the wing vein or cutting of toe nail from randomly selected five chickens depending on age and then transferred to sterile tubes containing anticoagulant (oxalate solution) at the rate of 1:10 for haematological examinations. All the haematological parameters as TEC, Hb, PCV and ESR were determined as per method. Recording of clotting time was done by cutting of toe nail for day old chicks and by puncturing the wing vein by a autoclix lancet for other aged chickens. When blood started to come out. a clean and dry glass slide used to take a drop of fresh blood from that place. The clotting time was then recorded in second. The biochemical parameters like glucose, cholesterol, urea and uric acid were studied using Reflotron auto analyzer in the Department of Physiology, BAU. The blood sample was drawn up using the Reflotron pipette avoiding the inclusion of air and this was applied as a drop to the center of the application zone without allowing the pipette tip to touch the zone. The strip was inserted horizontally into the instrument until hearing a click. Then the biochemical parameters appeared on the display and data were recorded. Data obtained were subjected to do statistical analysis. The results were expressed in mean, standard deviation and standard error were calculated according to the standard procedures.

Source of Information: BangI. J. Vet. Med. (2005).3 (1): 17-19.

Article Link (Online Journal): http://dx.doi.org/10.3329/bjvm.v3i1.11321

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The findings of the present work clearly indicated that age has got influential effect on the body haemopoietic system of the broiler birds as well as haematobiochemical values.

Knowledge Management: Journal