Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Effect of Nerium Oleander Poisoning on Blood of Male Guineapigs

M. G. A. Chowdhury
Thana Livestock Office, Kotwaly, Chittagong, Bangladesh.

Azizunnesai
Department of Medicine and Surgery, Chittagong Government Veterinary College, Pahartali, Chittagong, 4202, Bangladesh.

M. A. Hossain
Depanment of Parasitology and Pathology, Chittagong Government Veterinary College, Pahartali, Chittagong, 4202, Bangladesh.

Q. Hasan
Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh- 2202, Bangladesh.

Abstract/ Executive Summary:

Haematological changes were studied in 36 adult male guineapigs during the period from July to December 1994. These animals were divided into six equal groups (A to F), each group consisting of six animals. Each animal of group B to F was administered with a single oral dose of crude watery extract of sheath oleander @ 300, 450, 600, 750 and 900 mg/ kg body weight, respectively, whereas animals of group A which served as control. After administration of crude watery extract of 1/6 and 72 hours a significant increase of in total erythrocyte count (TEC), total leukocyte count (TLC) and haemoglobin (Hb). The maximum increase on TEC, TLC and Hb as 35. 51 and 14%, respectively, @ 750 mg/ kg body weight at 6 hr of administration of crude watery extract and the minimum value of TEC, TLC and Hb as 16.27 and 4%, respectively, with the dose rate of 300 mg/ kg body weight at the same time. The elevated haematological parameters returned to normal level within 7 days of administration.

Keywords: Haernatological parameters, Poisoning effect, Nerium oleander, Guineapigs

Location: Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh

Start Date: 00-07-1994

End Date: 00-12-1994

Program Area: Animal Health and Management

Commodity: Animal

Objectives:

To investigate the haematological changes by the effect of Nerium oleander poisoning in male guineapigs.

Methodology:

A total of 36 apparently healthy adult male guineapigs (Cavia cobaya) of nondescript breed and weighing between 700 to 900 g obtained from the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh were used in this study during the period from July to December 1994. All the guineapigs were kept under observation in order to acclimatize to the new environmental condition prior to the commencement of the experiment. All the guineapigs were maintained under good housing conditions and provided with normal feed and tap water ad libitum. Thirty gram fresh mature leaves of sheath oleander were collected from plant and finely grinded in a mortar and made the volume up to 300 ml. The mixture then filtered through a muslin cloth and the filtrate used as crude watery extract of sheath oleander for administration to the guineapigs. Guineapigs were randomly divided into six groups and each group comprising of 6 animals. The groups were designed as A, B, C, D, E and F. All the animals were weighed carefully and kept group-wise in 6 cases. Each animal of groups B to F were administered crude watery extract of sheath oleander in single oral doses of 300, 450, 600, 750, and 900 mg/ kg body weight respectively while animals of group A served as control. Blood samples from each of the experimental animal were collected directly from the heart of guineapigs in vials contained EDTA (ethylenediaminetetraacetic acid) as an anticoagulant at 1/6 and 72 hours, and 7 days of post administration of watery extract of oleander. Each of the collected blood sample was tested for total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin (Hb) and differential leukocyte count (only for neutrophil) as per method. All the data were analyzed statistically between controlled and treated values by using Student's 'test'.

Source of Information: Bangl. J. Vet. Med. (2005), 3 (1): 71-73.

Article Link (Online Journal): http://dx.doi.org/10.3329/bjvm.v3i1.11389

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The elevated haematological parameters returned to normal level within 7 days of administration of oleander extract indicated that the damage, if any caused by the Nerium oleander. There were minor haematological changes noticed after 30 minutes of extract administration (except 900 mg/kg body weight) which increased gradually and reached a maximally at the end of 6 hr and then decreased gradually and returned to normal level within 7 days of administration of leaves. In consonance with the time factor, the increased haematological changes were directly proportional to the doses.

Knowledge Management: Journal