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In Vitro Growth and Multiplication of a Hybrid Orchid (dendrobium Alba X Ascanda Dongtarm) with Different Concentration of Plant Growth Regulators

Md. Nurul Islam
Senior Scientific Officer (SSO)
Soil Resource Development Institute (SRDI), Regional Office, Rajshahi, Bangladesh.

Khondokar Md. Nasiruddin
Professor
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh

Md. Al Amin
Chief Scientific Officer
Division of Biotechnology, Bangladesh Agriculture Research Institute, Gazipur

Abstract/ Executive Summary:

In vitro growth and multiplication of a hybrid orchid was carried out to investigate the effect of different plant growth regulators on development of protocorm like bodies (PLBs) and subsequent plantlets of hybrid orchid. Protocorm like bodies (PLBs) were cultured on Murashige and Skoog (MS) media containing different concentration of NAA (0, 0.5, 1.0, 1.5 and 2.0 mg/l), IAA (0, 0.5, 1.0, 1.5 and 2.0 mg/l) and 0.5 mg/l of Kinetin in all cases were used. Among the different concentration treatments of NAA and IAA; and Kinetin concentration, maximum weight of PLBs (1.37g/explant) were noticed on MS medium supplemented with 1.5 mg/l NAA, and the highest number of plantlets (18.58/explant) were observed on MS medium containing 1.0 mg/l NAA. But maximum PLBs multiplication and or highest number of PLBs (28.50/explant) were found on MS medium supplemented with 2.0 mg/l IAA+0.5 mg/l of Kinetin

Keywords: Hybrid orchid, Protocorm like bodies, In vitro multiplication,Growth regulators

Location: Laboratory of Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur

Start Date: 00-08-2002

End Date: 00-03-2003

Program Area: Variety and Species

Commodity: Cactus

Objectives:

To investigate the effect of different plant growth regulators on development of protocorm like bodies (PLBs) and subsequent plantlets of hybrid orchid.

Methodology:

The experiment was carried out at the laboratory of Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur, during the period from August 2002 to March 2003 to investigate the effect of different plant growth regulators on in vitro growth of PLBs of a hybrid orchid. In this experiment, in vitro grown one month old PLBs were used as explant and growth medium was Muraskige and Skoog (1962) supplemented by plant growth regulators. The experiment was laid out in Completely Randomized Design (CRD) with 5 treatments and 4 replications. The treatments combinations were of Naphthalene Acetate NAA (0, 0.5, 1.0, 1.5 and 2.0 mg/l), Indole Acetic Acid (IAA) (0, 0.5, 1.0, 1.5 and 2.0 mg/l) and 0.5 mg/l of Kinetin in all cases were used with treatments. PLBs were cultured on MS medium supplemented with different concentrations of plant growth regulators NAA and IAA; and their combination by Kinetin concentration. One month old seven in vitro PLBs (Plate 1a) were placed in each culture vial. The culture vials were placed in a growth room and allowed to grow at 25?1 oC under 16 hour photoperiod illuminated with fluorescent tube of 2000- 3000 lux. Data were collected on the effect of different treatments of different parameter such as number of plantlets, number of rooted plantlets, and number of PLBs and weight of PLBs. The data were collected at 30 days interval. The treatment means of analyzed date were compared with LSD values.

Source of Information: Journal of Bioscience and Agriculture Research (JBAR), Vol. 1 (01): 27-33, 2014

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

It was revealed that for maximum weight of PLBs with 1.5 mg/l NAA is the best, while for rooted number of plantlets were found best with 1.0 mg/l of NAA concentration. But for maximum PLBs multiplication of the studied cross hybrid orchid (Dendrobium alba, Ascanda dongtarm) plant growth regulator combination 2.0 mg/l NAA + 0.5 mg/l Kinetin was found suitable among all other treatment.

Knowledge Management: Journal