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Fatty Acid Composition of Ripe Seed Oil of Nyctanthes Arbor-tristis L.

Mohammad Mizanur Rahman
Department of Chemistry, Jagannath University, Dhaka-1100, Bangladesh.

Sudhangshu Kumar Roy
Chemical Research Division, BCSIR Laboratories, Dr. Qudrat-i-Khuda Road, Dhaka-1205, Bangladesh.

Mohammad Shahjahan
Department of Chemistry, Jagannath University, Dhaka-1100, Bangladesh.

Abstract/ Executive Summary:

The fatty acid composition of the ripe seed oil of Nyctanthes arbor-tristis L. (Bengali: Seuli) were determined by GLC. The major constituent of the oil was found to be stearic acid, 39.06%. The relative percentages of other major fatty acids were found to be lauric acid, (4.46); linoleic acid, (7.89); oleic acid, (7.97). The yield of the seed oil was found to be 7.29% on extraction with pet-ether (b. p. 40°C - 60°C). Acid value of seed oil was found to be 55.44 and suggests that this oil is inedible.

Keywords: Nyctanthes arbor-tristis, Ripe seed oil, Fatty acid composition

Location: Gandaria area of Dhaka city in Bangladesh

Start Date: 00-02-2002

End Date: 00-03-2002

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To analysis fatty acid composition of ripe seed oil of Seuli plants

Methodology:

The ripe seeds of matured Seuli plants were collected from Gandaria area of Dhaka city in Bangladesh during the months of February to March, 2002. The collected seeds were cleaned, dried (70 ºC for 2 hours) and crushed mechanically. Physico-chemical characteristics viz. appearance, refractive index, acid value, iodine value and fatty oil of Seuli ripe seeds were determined as per standard method. Hundred gm of dried powdered samples of Seuli seeds were extracted with petroleum ether (40 - 60 ºC bp) in a Soxhlet extractor for 18 hrs. The evaporation of the extract was carried out with the help of a Rotary Vacuum Evaporator (Buchii, Switzerland) at bath temperature not exceeding 40 - 50 ºC. The oil sample was kept in nitrogen atmosphere in a refrigerator. The esterification of the oil was done with BF₃ - MeOH complex. Five mg of oil was taken in a reaction tube and BF₃CH₃ OH reagent (5 ml) was added. The mixture was boiled for 5 min. Hexane (5 ml) was added to it and boiled for further 1 min. After cooling the tube, a solution of saturated salt was added and vortexed. Then the upper layer containing methyl esters was transferred to a vial with anhydrous sodium sulfate at the bottom. Then the ester was filtered through syringe filter and transferred to a small vial (2 ml). The residual solvent was removed by blowing nitrogen gas and stored in a refrigerator before analysis by GLC. Standard fatty acid methyl esters (LIPID STANDARD; Sigma) were used for the ideºntification of the peaks. The standards comprised of the methyl esters of caprylic acid, nonanoic acid, capric acid, undecanoic acid, lauric acid, myristic acid, palmitic acid, linoleic acid, oleic acid, stearic acid, arachidic acid and behenic acids. The fatty acids in the experimental samples were identified by comparing their retention time and peak positions. Relative percentage of the fatty acids were also determined by comparing their peak areas (Peak area = ½ x peak height x peak width). The fatty acid methyl esters were quantified by gas-liquid chromatography method using a capillary column (2000 mm x 4 mm) equipped with a flame ionization detector (Pye Unicam 4500). Column packing was done with 10% diethylene glycol succinate on 100 - 120 mesh diatomic CAW with column temperature 100 ºC, detector temperature 220 ºC, hydrogen flow rate 4 ml/min and samples volume of injected 0.1 ml.

Source of Information: Journal of Bangladesh Academy of Sciences, Vol.35, No.1, 121-124, 2011, ISSN 0378-8121

Article Link (Online Journal): DOI: http://dx.doi.org/10.3329/jbas.v35i1.7977

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Higher percentage of stearic acid was found in the oil of ripe seeds and is the main component of the oil. The oil obtained form seuli seed does not satisfy the requirement as edible. It may be used for non-edible commercial and medicinal purposes.

Knowledge Management: Journal