Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Mitigation of the Adverse Effects of Soil Salinity in Rice Using Exogenous Proline and Organic Manure

Sukanta Dhar
Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Mohammad Golam Kibria
Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Mazibur Rahman
Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Dr. Md. Anamul Hoque
Professor
Department of Soil Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

Salinity causes cellular damage and limits crop productivity. Accumulation of organic compound is one of the major adaptive mechanisms for salinity tolerance in plants. The main objective of the present study was to mitigate the adverse effects of soil salinity in rice through organic amendments like proline and organic manure. The field experiments were conducted at the farmer’s field of Batiaghata upazilla under Khulna district with aman rice. The soil was silty clay loam having pH 7.2, EC 6.6 dS/m, CEC 26 meq/100 g soil and organic matter 0.84%. Rice variety BR 23 was used as a test crop. There were 15 treatment combinations with different doses of proline and organic manures. Recommended doses of N, P, K, S and Zn fertilizers were applied to all the experimental plots. Proline was applied as foliar spray at seedling and/or vegetative stages. Organic manures were added to soils during final land preparation. The experiments were laid out in a randomized complete block design with three replications. Significant reductions in growth and yield of rice were observed under saline conditions. Application of both proline and organic manures significantly increased growth, yield contributing characters, and grain and straw yields of rice under salinity conditions. There were no significant variations in growth and yield of rice due to use of different doses of proline and organic manure. Increased nutrient uptake and K⁺/Na⁺ ratio were observed in rice due to proline as well as organic manure application under saline conditions. The present study suggests that exogenous proline or organic manure confers tolerance to salinity in rice by increasing K⁺/Na⁺ ratio and nutrient uptake.

Keywords: Salinity,Proline, Organic manure,Rice

Location: Batiaghata upazilla under Khulna district

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Soil salinity, Rice, Manures

Objectives:

To investigate the improvement of soil salinity tolerance in rice by organic manure (farmyard manure and poultry manure) and proline application.

Methodology:

The field experiments were carried out at the farmer’s field of Batiaghata upazilla under Khulna district to investigate the improvement of soil salinity tolerance in rice by organic manure (farmyard manure and poultry manure) and proline application. The soil of Batiaghata upazilla was extremely saline due to higher EC value (6.4 dS/m) and due to this salinity rice production shows significant reduction in yield. The experiment was conducted here so that the performance of exogenous proline and organic manure application could be assessed under field condition. The experiment was laid out in a randomized complete block design (RCBD) with three replications. Rice cultivar BR23 was used as a test crop in this study. The total numbers of plots used in this study were 45. The unit plot size was 4m×2.5m and the plots were separated from each other by ails (1m). Treatments were randomly distributed within the block. Soils were amended with different doses of farmyard manure (FYM) and poultry manure (PM). In another experiment, plants were treated with different concentrations of proline at seedling and vegetative stages. In experiment 1, the treatments were T₀=Control (no manure), T₁= FYM (5 t ha^-1), T₂= FYM (10 t ha^-1), T₃= PM (4 t ha^-1), T₄= PM (8 t ha^-1). In second experiment, there were ten treatments including control and those were T₀= Control (no proline), T₁= 25 mM proline at seedling stage, T₂= 25 mM proline at vegetative stage, T₃= 25 mM proline at seedling and vegetative stages, T₄= 50 mM proline at seedling stage, T₅= 50 mM proline at vegetative stage, T₆= 50 mM proline at seedling and vegetative stages, T₇= 100 mM proline at seedling stage, T₈= 100 mM proline at vegetative stage, T₉= 100 mM proline at seedling and vegetative stages. Recommended doses of Urea, TSP, MP, gypsum and zinc sulphate were applied as the source of N, P, K, S and Zn (BARC, 2012). Recommended doses of fertilizer were applied to all experimental plots during final land preparation. Urea was applied in three split doses; first dose was applied after ten days of transplanting, second dose was applied at active tillering stage, and third dose was applied at panicle initiation stage. Weeding and other management practices were performed as and when necessary. Birtako 10g (2g dissolved in 10 L water) was applied as plant protection measures when insect, pests and diseases were appeared. Proline solutions were sprayed over plant leaves with the help of a sprayer. For making 25 mM, 50 mM and 100 mM proline solutions, 2.88 g, 5.76 g and 11.52 g of proline powder were dissolved in 1000 ml of water and 1 ml of Tween-20 was properly mixed with it respectively. Tween-20 was used as a sticky substance which helps proline solution’s droplet, maintaining a close contact with plant leaves. Maturity of crop was determined when about 90% grains became golden yellow. The crop was harvested on 29 December, 2014 at 120 days after transplanting. Grain and straw yield as well as plant growth parameters (Plant height, number of effective tillers per hill, panicle length and 1000 grains weight) were recorded after harvesting. Grain and straw samples of maize were analyzed for N, P, K, S and Na contents following standard method. Data were analyzed statistically using analysis of variance (ANOVA) to examine the treatment effects. The mean differences were adjudged by Duncan’s Multiple Range Test (DMRT) and ranking was indicated by letters.

Source of Information: Asian J. Med. Biol. Res. 2015, 1 (3), 478-486; ISSN 2411-4472 (Print) 2412-5571 (Online)

Article Link (Online Journal): doi: 10.3329/ajmbr.v1i3.26465, www.ebupress.com/journal/ajmbr

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Salt stress caused a significant decrease in growth and yield of rice. Foliar application of proline and organic manures both FYM and PM significantly improved growth and yield of rice by mitigating the inhibitory effects of salinity stress. Application of poultry manure (8 t ha^-1) or farm yard manure (10 t ha^-1) showed higher yield of rice. Poultry manure showed higher yield performance than FYM application. Besides, 100 mM proline application at both seedling and vegetative stages showed better yield performance compared to other treatments. In addition to field research work, better understanding about physiological and bio-molecular mechanisms of proline will be helpful for plant breeders to develop stress-tolerant crop varieties. On the other hand, organic manures like FYM and PM are more available and less expensive.

Knowledge Management: Journal