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Comparative Surveillance of Parasitic Infestation in Channa Punctatus (osteichthys: Channidae) Collected from Open and Closed Water in Sylhet, Bangladesh

Md. Faruque Miah
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

Mitu Deb
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

Hazrat Ali
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh

M. M. A. Quddus
Department of Zoology, University of Dhaka, Bangladesh

Kawser Ahmed
Department of Oceanography, University of Dhaka, Bangladesh

Abstract/ Executive Summary:

This study deals with the investigation of parasitic infection in Channa punctatus collected from open and closed water in Sylhet, Bangladesh. Skins, gills and intestine of the host were observed for finding parasites. In total 253 parasites were identified under 26 species from 40 experimental fish. 12 species of ectoparasites and 13 species of endoparasites were recorded with 1 more species of both ecto and endo parasitic activities in Channa punctatus. Different types of parasites such as protozoa, platyhelminth, nematoda, acanthocephala, arthropoda, gastrotricha and rotifer were found from experimental fish. The overall incidence of infection in Channa punctatus was higher (60.00%) of protozoan parasites. The highest incidence of infection of protozoan parasites was recorded as 60.00% in open water samples. On the other hand, the highest incidence of infection in closed water was recorded as 75% of platyhelminth parasites. Furthermore, the intensity of infection by protozoan species was recorded in open and closed water fish as 2.92 and 4.83 respectively while the intensity of infection by platyhelminthes species found was 1.00 and 1.87 in open and closed water respectively. Finally, two new species (Rotifer sp. and Chaetonotus sp.) were assumed to be new parasites in Channa punctatus.

Keywords: Surveillance, Infestation, Channa punctatus, Open water, Closed water

Location: Sylhet, Bangladesh

Start Date: 00-08-2011

End Date: 00-11-2011

Program Area: Animal Health and Management

Commodity: Fish

Objectives:

To investigate the different parasite communities, and to find out the prevalence and intensity of infestation in Channa punctatus with different habitat of varying water quality in Sylhet.

Methodology:

Study Location and Sampling: A total of 40 individuals of Channa punctatus were collected randomly from both closed (20 fish) and open water (20 fish) at different times from different locations of Sylhet, during the period of August to November, 2011. Collected fish samples were transferred to the Animal Biotechnology Laboratory (ABL) of the Department of Genetic Engineering and Biotechnology (GEB), Shahjalal University of Science and Technology (SUST), Sylhet, Bangladesh. Experimental Procedure: Fish samples were taken in live condition, killed by hand and examined immediately for parasitological study using compound microscope. A clean spatula was held to the body of each individual and it was drawn backwards towards the tail in a smooth movement and lifting off a small amount of mucous from the different sites of the body for investigating parasites from skin. Later on, for each sites, mucous scrapings placed on a clean glass slides and examined under the 4x, 10x and 40x lenses of the compound microscope for observing the presence of parasites. In gill biopsy, a fine pair of scissors was used to cut open the operculum from both sides to reveal the opercular cavity. Gill filaments were taken out by cutting off the two ends of the gill arches, and kept on petridishes with the saline solution of NaCl (70%). Furthermore, small sample of gills was made by splitting up gill filaments using fine scissors and observed under microscope. Each fish was dissected with a fine scissors used to make an incision along the mid-ventral line of the body to find out the parasites from the internal organs. All other organs (stomach, heart, liver, kidney, spleen and gall bladder) were shredded by using forceps and needles, and separated from intestine. The intestines were carefully opened out by an incision from the body and put into a petridishes with the physiological saline solution (NaCl 70%). Sometimes larger nematodes (roundworms) were visible in naked-eyes lying in the body cavity with their heads buried in the intestines and were quickly isolated using forceps, fixed on glass slide with glycerin. Saline solution of the intestine was made by scrapping of the epithelial tissues using fine scissors onto petridishes and viewed under several microscopic lenses. All the isolated parasites were fixed in glass slides with glycerin. Some parasites are virtually transparent, smaller in size and unable to move in saline solution of target organs. In that case, we had to scan slowly by adjusting the microscope condenser power higher to lower. And viewed the saline solution under dark-filed to identify parasites present in the target organs. Microscopic picture of all the isolated parasites were taken by converted microscope as well as high megapixel camera and the parasites were identified by the image analysis. Analysis of Parasitic Infestation: The analysis of parasitic infestation for finding the incidence, intensity, density and index were carried out.

Source of Information: Advances in Zoology and Botany 1(1): 17-23, 2013

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The highest incidence of infection of protozoan parasites was recorded as 60.00% in open water samples. On the other hand, the highest incidence of infection in closed water was recorded as 75% of platyhelminth parasites. Furthermore, the intensity of infection by protozoan species was recorded in open and closed water fish as 2.92 and 4.83 respectively while the intensity of infection by platyhelminthes species found was 1.00 and 1.87 in open and closed water respectively. Finally, two new species (Rotifer sp. and Chaetonotus sp.) were assumed to be new parasites in Channa punctatus.

Knowledge Management: Journal