Md. Abul Kalam Azad
Chief Scientific Officer
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh 2202, Bangladesh
Md. Shah-E-Alam
Professor
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
Md. Abdul Hamid
Ex. Director General
Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh 2202, Bangladesh
Mohd Y. Rafii
Professor
Institute of Tropical Agriculture, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia
M. A.Malek
Chief Scientific Officer
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh 2202, Bangladesh
Combining ability, Pod yield, Salinity stress, Groundnut
The glasshouse of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh
Crop-Soil-Water Management
Materials: A 6 × 6 F1 diallel population without reciprocal was used in this study. The parents of this population were discriminated as being salt tolerant/sensitive based on pod number and yield under salt stress. Four salt tolerant mutants/cultivars: M6/15/70-19, BinaChinabadam-1, M6/25/64-82, and “Binachinabadam-2” and 2 sensitive cultivars: Dacca-1 and Zhingabadam were crossed following the method of Kumar and Patel with some modifications. It is important to mention here that the parent of BinaChinabadam-1, Binachinabadam-2”, M6/15/70-19, and M6/25/64-82 was Mut-6. Mutant-6 was derived by irradiating the seeds of Dacca-1, a local land race; thus, Dacca-1 is the common parent of BinaChinabadam-1, BinaChinabadam-2, M6/15/70-19, and M6/25/64-82. Experimental Site, Experiment Design, and Plant: Culture- The experiment was conducted in the glasshouse of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, with temperature and relative humidity ranges of 25–350C and 70–90%, respectively. The study was set up as a completely randomized design with four replications, under 8 dS/m salinity level, imposed during flowering till harvest stages as flowering stage is the most sensitive stage to salinity stress. Five pre-germinated seeds of each parent and cross were sown on February 11, 2007 in earthen pots of size 27 × 22 cm, lined with polyethylene sheet and filled with 8.0 kg soil mixture, prepared with sandy loam soil and well-rotted farm yard manure in a 1:1 ratio. The fertilizer needed for each pot was determined following the Fertilizer Recommendation Guide-2005. The total amount of nitrogen, phosphorus, potassium, sulphur, and zinc was applied in the form of urea, triple super phosphate (TSP), muriate of potash (MP), gypsum, and zinc sulphate. These were mixed thoroughly with the soil in each pot before sowing. When the plants were established, only three healthy plants were kept in each pot. The pots were kept free from weeds and protected from insect pests and diseases by spraying appropriate pesticides as and when necessary. Imposing Salinity and Data Recording: Saline water was synthesized by using a mixture of different salts: 50% NaCl, 15% Na2SO4, 10% NaHCO3, CaCl2, and MgCl2 together with 5% MgSO4 by weight to simulate saline ground water of saline areas of Bangladesh. A stock solution was prepared by dissolving 50 grams of the salt mixture in 1 liter of tap water. The salinity of the stock solution was then adjusted to 80 dS/m. The total amount of stock solution needed to raise 8 dS/m salinity of the soil mixture was estimated with the following equation V1S1=V2S2. Volume of water was determined by dividing the weight of water by its density (0.98 g/cc). The estimated amount of stock solution was then diluted to the desired salinity levels by adding tap water and then applied from flowering until harvest. The total amount of saline water for the respective treatments was applied in installments. At each installment, 0.5 to 1.0 liter saline water was applied so that the moisture content of the pots remained between 70 and 80% of PAW. For the control, the same amount of tap water was applied. All plants in a pot were uprooted at full maturity and washed with running tap water. Plant height, branch number, and pod number were recorded before sun drying. After sun drying, pod yield per plant was recorded. There were altogether 12 plants in each F1 populations, three plants in a pot and 4 such pots in each population. Genetic Analysis: Combining ability analysis was performed. Data were analyzed statistically using MSTAT-C software.
The Scientific World Journal, Volume 2014, Article ID 589586, 7 pages
Journal