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Research Detail

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MA Wadud
Scientific Officer
SRC, Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogra

AHF Fahim
Scientific Officer
SRC, Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogra

MS Naher
Scientific Officer
SRC, Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogra

MB Sarker
Scientific Officer
SRC, Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogra

MJ Uddin
Scientific Officer
BSRI

MR Humauan
Scientific Officer
RARS, BARI, Ishurdi, Pabna, Bangladesh

The experiment was conducted at Spices Research Centre (SRC), Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogra during 2013 to 2015 to determine the best management option against grey leaf spot disease of bay leaf. Five fungicides (Tilt 250 EC, Combi-2, Bavistin DF, Sunvit 50WP and Nativo 75 WG), one botanical extract (eucalyptus leaf extract) and one chemical fertilizer (MOP) along with control were used in this study. The experiment was laid out in Randomized Complete Block (RCB) design with three replications. A total of four sprays were applied at 15 days intervals starting from first initiation of disease symptoms. All fungicides and botanical extract were found effective significantly in controlling grey leaf spot disease over control. Lowest disease incidence (16.00% in 2013-14 and 12.60% in 2014-15) was recorded in T2 (Tilt 250 EC @ 0.1%) treatment that was statistically similar with T7 (Tilt 250 EC @ 0.1% + top dressing of MOP @ 50g/plant) treatment and the highest disease incidence (44.32% in 2013-14 and 41.75% in 2014-15) was recorded in T8 (Control) treatment. Lowest disease severity (8.54% in 2013-14 and 8.45% in 2014-15) was recorded from T2 treatment and the highest disease severity was observed in T8 treatment. Among the fungicides, Tilt 250 EC @ 0.1% decreased the disease severity as well as increased the fresh leaf yield (21.14 kg/plant in 2013-14 and 23.81 kg/plant in 2014-15). Based on two year results, Tilt 250 EC @ 0.1% alone or combination with MOP @ 50 g/plant will recommended for the management of grey leaf spot disease of bay leaf without hampering the fresh leaf yield.

  Bay leaf, Grey leaf spot, Fungicide, Botanical extract, Disease incidence, Disease severity
  Spices Research Centre (SPC), Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogra
  00-00-2013
  00-00-2015
  Pest Management
  Diseases, Extract (plant, seed)

To determine the effective management option for controlling grey leaf spot disease of bay leaf.

The experiment was conducted at  Spices Research Centre (SPC), Bangladesh Agricultural Research Institute (BARI), Shibganj, Bogra during 2013-14 and 2014-15. The land was medium high and the soil was clay loam in texture. Medium aged (about 8-10 years old) bay leaf plants were taken as test crop. For this, twenty four small sized (about 8-10 years old) bay leaf plants were selected for treatment application, observation and data recording. The weeds and stubbles of previous crops were collected and removed from the soil. The soil was clay loam in texture. The land was fertilized with cow dung 10 kg/plant, and TSP300, MOP300 Urea200, Gypsum100, Boric Acid20, Zinc Sulphate30 g/plant, respectively. The entire quantity of cow dung, TSP, MOP, 50% of Urea, Gypsum, Boric Acid and Zinc  Sulphate were used as basal applicatin during 2013 and 2014. The experiment was laid out in RCB design with three replications. Advanced bay leaf line (CTB 002) was used. Five fungicides, one botanical extract, top dressing of MOP fertilizer with fungicide and one control treatment were used. The treatments were, T1 = 5% water extract of eucalyptus leaves; T2 = Tilt 250 EC (Propiconazole) @ 0.1%; T3 = Combi-2 (15% Propiconazole + 15% Difenoconazole) @ 0.1%; T4= Bavistin DF (50% Carbendazim) @ 0.1%; T5 = Sunvit 50WP (Copper Oxychloride) @ 0.5%; T6 = Nativo 75 WG (25% Trifloxystrobin + 50% Tebuconazole) @ 0.05%; T7 = Tilt 250 EC (Propiconazole) @ 0.1% + top dressing of MOP @ 50g/plant and T8 = Control. Top dressing of MOP was applied into 4 (four) equal installments. Three times irrigation were required for the growth and development of the trees. Rest 50% of urea was applied at 25 days after application of basal dose followed by irrigation. The treatments were applied 4 times at 15 days intervals starting from first appearance of the disease  symptoms.  Data  were recorded  from ten (10) randomly selected tertiary branches of each selected plants at 15 days interval after application of treatment (three trees in each replicate).  Data on number of fresh leaves per tertiary branch, number of diseased leaves per tertiary branch, disease incidence, percent disease severity and percent fungicide efficacy were recorded by counting spotted leaves and visual estimation, respectively. Fresh leaf yield (kg/plant) and yield increased over control were recorded after harvesting the leaves during first week of June. Twenty leaves were randomized collected from randomly selected ten tertiary branches of each tree. The infection on each leaf was rated by using a numerical index (containing 5 infection category) ranged from 0 = Healthy, which represented no infection on the leaf, 1= ¼ leaf area covered by infection, 2 = 1/3 leaf area covered by infection, 3 = less than ½ leaf area but more than 1/3 leaf area covered by infection and  4 = More than ½ leaf area covered by infection or the leaf is destroyed. Disease severity was calculated following the equation developed by Tow send and Heuberger (1943). % Disease severity = [(n × v)/4N] × 100; Where, n = Number of leaves within infection category. v = Numerical value of each category. N = Total number of leaves. Fungicide efficacy was calculated by using  Abbott equation (Frolich, 1979) % Fungicide efficacy = C - T/C x 100; Where, C = Disease severity in the control. T = Disease severity in the treatment. All the recorded data were analyzed statistically  to find out the level of significance and the variations among the data were compared by following Duncan’s Multiple Range Test (Gomez and Gomez, 1984).

  Eco-friendly Agril. J. 10(07):90-95, 2017 (July)
  
Funding Source:
1.   Budget:  
  

The present study showed that among the  five test fungicides, one botanical extract and one fertilizer (MOP); Tilt 250 EC @ 0.1% alone or combination with MOP @ 50 g/plant gave better control against the disease. Based on two years study it revealed that Tilt 250 EC @ 0.1% alone or combination with MOP @ 50 g/plant provided better  performance  against grey leaf spot disease control of bay leaf as well   as increasing the fresh leaf yield.

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