A pot experiment with Stevia rebaudiana (Bertoni) was carried out by combined application of vermicompost (VC) and chemical fertilizer (CF) on the growth, leaf biomass yield, stevioside content of stevia and postharvest soil fertility in response to their treatments. The experiment was conducted at the net house of the Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh, during February to May, 2013. Only acid soil was considered for this experiment. Approximately 40 kg acid soils were collected from 0-15 cm depth of selected fallow land of Madhupur under Tangail district of Bangladesh. After proper cleaning, the soil was sieved through a 2 mm sieve and 500 g sieved soil from each source was preserved for chemical analysis. The physicochemical analysis of processed soil, vermicompost and postharvest soil was performed in different laboratories of the Department of Agricultural Chemistry, Biochemistry, Professor Muhammed Hussain Central Laboratory (PMHCL), BAU, Mymensingh and SRDI Regional Laboratory, Dhaka as reported by Page et al. (1982). All the chemicals used were analytical reagent (AR) grade. Stevia seedling was raised in broad earthen pots (30 cm × 23 cm) containing 8 kg processed soil per pot. Plant samples were procured from brac Biotechnology Laboratory, Joydebpur, Gazipur. As basal doses, N, P, K, S, Zn and B fertilizers were applied as rates of 250, 100, 200, 30, 3 and 1 kg ha−1 from TSP, MoP, gypsum, zinc sulphate and boric acid, respectively. Sulphur rate was adjusted from both gypsum and zinc sulphate. Six experimental treatments viz., VC0 + CF0 (No fertilizer), VC0+CF100 (VC @ 0 t ha−1 plus 100% CF), VC5+CF50 (VC @ 5 t ha−1 plus 50% CF), VC5+CF75 (VC @ 5 t ha−1 plus 75% CF), VC7.5+CF50 (VC @ 7.5 t ha−1 plus 50% CF), VC7.5+CF75 (VC @ 7.5 t ha−1 plus 75% CF) were used. The experiment was laid out in completely randomized design (CRD) with three replications using total 18 earthen pots. Intercultural operations were done as and when necessary. Data were collected at 15, 30, 45 and 60 days after planting (DAP) and was harvested at 60 DAP. After harvesting the crop, leaf samples were processed and dried at 60°C for 72 hours, weighed, ground and stored. Different growth parameters like plant height, branches plant−1, leaves plant−1, leaf area plant−1, fresh and dry leaf weight (g plant−1) of stevia were studied. Stevioside content of stevia leaf was determined following the standard method (Kolb et al., 2001). The concentrations of N, P, K and S in stevia were analyzed by standausing standard method of analysis described by Steel et al. (1997). Analysis of variance (ANOVA) was done following the principle of F-statistics and the mean values were separated by Latin Square Design (LSD) (Gomez and Gomez, 1984). rd methods (Tandon, 1995). Nutrient uptake was calculated from their concentrations and corresponding dry leaf yield. The obtained results were subjected to statistical analysis