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Effects of Soybean and Rice Bran Oil on Hemato- Biochemical Parameters in Mice

Nafis Islam
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Md. Shahidul Islam
Department of Physiology and Pharmacology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh

Md. Jahangir Alam
Veterinary Surgeon, Department of Livestock Service, Ministry of Fisheries and Livestock, GoB, Bangladesh

Md. Kamrul Islam
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Nazim Ahmad
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The study was conducted on “Swiss Albino” mice fed with additional supplementation of soybean oil and rice bran oil to observe the effects on hematological (total erythrocyte count and hemoglobin concentration) and biochemical parameters (total serum cholesterol, triglycerides, high density lipoproteins and uric acid). A total of 30, 6-8 weeks old mice were randomly divided into 3 equal groups (n=10) as A, B and C. Group A was considered as control (fed only commercial ration), group B was supplemented with rice bran oil and group C treated with soybean oil respectively in addition to commercial ration for 60 days. At the end of feeding trial the mice were sacrificed for analysis of hemato- biochemical parameters. The total erythrocyte count and hemoglobin concentration were increased significantly (P<0.05) in group B and C compared to control group A and the highest values was recorded in soybean oil group C. The total serum cholesterol, triglycerides, HDL and uric acid were increased significantly (P<0.05) in both rice bran oil and soybean oil group compared to control group. It is concluded that some hemato-biochemical parameters of blood in the mice are affected by rice bran and soybean oil enriched diet. Though, oils and fats are detrimental to health but to evaluate the effects of rice bran and soybean oil, further studies with more animals and some other parameters like liver function, kidney function may be conducted.

Keywords: Mice, Soybean oil, Bran oil, Hematological parameters, Biochemical values

Location: Department of Physiology, Bangladesh Agricultural University (BAU), Mymensingh, Bangladesh

Start Date:

End Date:

Program Area: Risk Management in Agriculture

Commodity: Animal, Soybean

Objectives:

The effect of rice bran and soybean oil on hematological parameters like total erythrocyte count (TEC) and hemoglobin (Hb) concentration of mice.
The effect of rice bran and soybean oil on lipid profiles as total serum cholesterol, triglycerides and high density lipoprotein (HDL) in mice.
To study the effect of rice bran and soybean oil on serum uric acid in mice.

Methodology:

The experiment was conducted in the Department of Physiology, Bangladesh Agricultural University (BAU), Mymensingh. Twenty Swiss Albino mice (Mus musculus) aging 6-8 weeks and an average body weight of 20-25 gm were used. Before being used in the experiment, mice were adapted for 7 days in order for them to get used to the new environment. The mice were randomly divided into 3 equal groups (n=10) as A, B and C Group A was considered control fed on standard broiler pellet (5 gm/mouse/day) and fresh drinking water. Group B and group C were fed on standard broiler pellet enriched with rice bran oil and soybean oil for a period of 60 days. All groups were housed in a compartmentalized rectangular metallic cages (9x 11x7 cubic inches) wrapped with wire mesh. The cages were kept in well ventilated room at 28.2°C and a relative humidity of 70-80% with natural day light. The experimental laboratory was cleaned and washed at a regular interval. Commercial Broiler pellet (HI-PRO-VITE feed), rice bran oil and soybean oil were purchased from local market and were supplied as group wise. The diet was prepared on daily basis and supplied as 5 gm/mice/day and water was supplied adlibitum in all groups. Group-A: Control and fed with broiler pellet; Group- B: Broiler pellet+ Rice bran oil (1000 gm : 25 ml); Group-C: Broiler pellet + Soybean oil (1000 gm : 25 ml). On day 60, blood samples were collected by sacrificing the mice. The abdominal cavity and thoracic cavity were opened and the blood was collected by a syringe directly from the heart. About 2 ml of blood was collected in the sterile glass test tube. The blood containing syringe was placed in upright slanting position at room temperature for 6 hours. The tubes were then incubated overnight in the refrigerator (4°C). The serum samples were separated and centrifuged to get rid of unwanted blood cells where necessary. Serum samples were stored in capped tube at -20°C for until analysis. The counting and calculation of RBC were performed as per methods indicated by Ghai (1999) and the result was expressed in million/mm3. The hemoglobin estimation was determined as per method described by Ghai (1999) and the result was expressed in gm/dl. The biochemical parameters of serum like Total Cholesterol, Triglyceride, HDL and uric acid were estimated.?The cholesterol was determined using the procedure described by Trinder (1969). The result was expressed in mg/dl. The triglyceride of blood serum was determined by Biochemistry Humalyzer-3000 (Human type, Germany) according to the technique described by Trinder (1969). The result was expressed in mg/dl. The concentration of serum HDL cholesterol was estimated with the incubation of supernatant of serum sample and reagent mixture in Reflectron Humalyzer 3000 (Human type, Germany) and then placing the mixture in the Reflection® against the blank reagent. The result was expressed in mg/dl. Both reagent and sample brought at room temperature and mixed 1.0 ml reagent with 25µl sample in test tube. Let waited for 10 minutes and poured the mixture in cuvette. The spectrophotometer was fixed at 500 nm and recorded the reading. Used reagent contains different types of enzymes. Uric acid is oxidized by uricase to allantoin with the formation of hydrogen peroxide. This hydrogen peroxide oxidized 4-aminopyrine to quinoneimine which is proportional to the concentration of uric acid in the sample. The reading was divided by standard value and result was multiplied by 6mg/dl. So the result was expressed as mg/dl. The hematological and biochemical parameters of mice corresponding to the different levels of rice bran oil and soybean oil supplementation are compared and performing by Student t’ Test (SPSS, 16 version).

Source of Information: Res. Agric. Livest. Fish. Vol. 5, No. 3, December 2018 : 365-372

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Hemato-biochemical parameters of blood in the mice are significantly affected by rice bran and soybean oil enriched diet.

Knowledge Management: Journal