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Research Detail

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M.M.Islam
Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

N. Muhammad
Department of Agricultural Extension, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

N.B. Islam
Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

M.S. Forid
Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.

A.T.M.S. Islam
Department of Plant Pathology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

An experiment was conducted in IRT Building of Hajee Mohammad Danesh Science and Technology University, Dinajpur during 20 March to 20 June, 2016 to evaluate the effects of biotic and abiotic factors on seed quality of maize seed. Three types of containers namely plastic container, polythene bag and gunny bag were used as experimental treatment. The containers were stored at room temperature and ambient relative humidity around three months. The experiment was laid out in Completely Randomized Design (CRD) with three replications. Seed quality factors viz., moisture content, germination percentage, vigor index, percentage of abnormal seedling, fresh seed and dead seed were recorded every 15 days interval. The initial moisture content of seed in plastic container, poly bag, and gunny bag were 9.46%, 10.22% and 11.87%, respectively, but it was increased with increasing storage time after 60 days (10.82%, 11.13% and 13.00%). The germination percentage was higher at 15 days after storage (DAS) for different containers like plastic containers (85%), poly bag (81%) and gunny bag (75%) than after 60 DAS (75%, 71% and 63%), respectively, on sand substrate. The percentage of abnormal seedling was increased from 11 to l7%, 13 to 19%, and 15 to 20% in plastic container, poly bag, and gunny bag, respectively, from 15 DAS to 60 DAS. The percentage of dead seed was increased from 2 to 3% and 4 to 5% in plastic container and gunny bag,respectively. The vigor indexes of seedling were 14.58, 12.85 and 10.92 in plastic container, poly bag and gunny bag, respectively at 15 DAS and it was decreased (10.39, 10.26 and 10.08) at 60 DAS, respectively. The result revealed that the germination percentage decreased with the increase in storage period. The study also indicated that initial moisture and storage container has significant role on the quality of maize seeds and plastic container is the best one for storing the seeds with better benefits.

  Seed quality, Maize, Storage containers, Storage periods
  Hajee Mohammad Danesh Science and Technology University, Dinajpur
  20-03-2016
  20-06-2016
  Seed Technology
  Maize

1. To identify the best container for storing maize seed with the effect of biotic and abiotic factors on the storing performance and quality of maize seeds and

2. To determine the interaction between storage container & initial moisture content of maize seed on the storage legibility.

The study was conducted at the IRT Building of the Hajee Mohammad Danesh Science & Technology University, Dinajpur (HSTU) during 20 March to 20 June, 2016 for around Three months to observe the effects of variation in Maize seed quality preserved in different seed containers. The experiment was conducted in the room temperature with three seed storage containers. The following steps were considered during the study period. Maize seeds of local variety (Barnali) were collected from the local market and used in this experiment. Seeds were kept in three supplied storage containers and stored at room temperature and RH for 2 months. Thousand seed weight was weighed. The following two sets of treatments were included in the experiment: Storage material: 3 i) Plastic container ii) Poly bag iii) Gunny bag; Storage period: 4 i) l5 days ii) 30 days iii) 45 days iv) 60 days; The experiment was laid out in Completely Randomized Design (CRD) with three replications. Sampling was carried out at l5 day’s intervals up to 60 days i.e. 4 times. At each sampling, samples were taken randomly from each storage container. During the storage period Seed samples were taken every fortnightly from the containers for determination of change of moisture content (Wet basis), germination percentage, presence of insect and others activities. Moisture content was determined by using high constant temperature oven method following International Rules for Seed Testing (Anonymous 1999) in the Agronomy Laboratory of HSTU. 5g of seeds from each 3 containers were taken and poured in a small container with cover and kept in an oven maintained at a temperature of 1300C to 1330C for a period of 1 hour. The moisture content of seeds (wet basis) was determined by the following formula (Anonymous 1999). MC (%) = M2-M3/M2-M1 x 100; M1 = Wt. of container + cover; M2 = Wt. of container + cover + Maize seed before drying; M3 = Wt. of container + cover + Maize seed after drying. Similar procedure was performed every fortnight from beginning of storage. Germination test was conducted using sand as substratum. The sand was sieved to discard particles bigger than 0.8 mm and smaller than 0.05 mm in diameter. Rectangular plastic boxes were used to put the sand. For every test new sand was used. Seed was placed on a uniform layer of moist sand and then covered to a depth of 10 mm with sand, which was left loose. 100 seeds were planted in each plastic tray and replicated three times. The plastic trays with seed were incubated at room temperature and irrigated at every odd day. After 8 days, germination percentage was recorded. The normal seedlings and abnormal seedlings and ungerminated seed were classified according to the prescribed ru1es given by ISTA. Germination (%) = Number of seeds germination/ Number of seeds tested x 100;  Seeds were stored at room condition in different containers and germination test was performed every 8 days of interval beginning from collection of seeds up to 40 days to observe the rate of natural aging of Maize seeds stored in different containers. Seedlings obtained from standard germination test were used for seedling evaluation. Normal or abnormal seedlings were classified according to the rules of ISTA (1993). Seedling shoot and root length was measured  on day I of the germination test. Ten plant samples from each plastic pot were harvested and shoot and root length of individual plant was recorded. For observation of biotic factor that influence seed quality, 20 g of Maize seeds were taken and observed the numbers and kind of insects or pathogen were presented. Reading of dry bulb and weight bulb temperature and relative humidity of inside the room and outside the room were recorded in 10.00 am and 4.00 pm every day. Amount of rainfall and maximum and minimum temperature were collected from Wheat Research Center, Nosipur, Dinajpur.

  Int. J. Sustain. Crop Prod. 11(3):20-28 (August 2016)
  http://ggfjournals.com/e-journals archive
Funding Source:
1.   Budget:  
  

From the overall results it may be concluded that improved indoor containers like plastic container gave quality seeds rather than poly bag and Gunny bag during storage period. Gunny bag is not safe for maize seed storage for long time than plastic container and poly bag due to the rate of moisture absorbance was higher in gunny bag than plastic container and poly bag.

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