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Research Detail

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Pankaz Biswas
Department of Seed Science and Technology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, Bangladesh

M. Abdul Karim
Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, Bangladesh

Golam Kabir
Department of Agronomy, Bangladesh Agricultural University, Mymensingh, Bangladesh

M. Moynul Haque
Department of Seed Science and Technology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur, Bangladesh

An investigation was carried out at the experimental field of the Department of Agronomy of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during January 2012 through April 2012, to characterize different plant parameters of thirteen dwarf soybean (Glycine max L.) genotypes. A wide diversity among the genotypes in their physio-morphological characters including yield was recorded. Genotypes varied from days 36 in G00221 to 40 days in G00197 to initiation of 50% flowering. Genotypes G00042 took the longer time (80.7 days) for maturity, where G00351 took the shortest time (68.67 days). Pods number per plant was the highest (60.67) in G00207, while the lowest (15.04) in G00041. The highest number of seeds per pod (2.087) was obtained from G00046, while G00207 had the least (1.657). Weight of 100-seed was the maximum (16.34 g) in G00041and that was the minimum (10.55 g) in G00166.Among all the genotypes, G00207 produced the highest seed yield (4.23 t/ha). Contrary genotypes G00046 produced the lowest seed yield (1.43t/ha). The germination percentage of the thirteen soybean genotypes varied from 76.00% (G00042) to 86.66% (G00166). The tetrazolium-test showed that the highest viable seed (91.67%) was in the genotype G00166 and the lowest (85.67%) was in G00042. Electrical conductivity test showed that genotype G00204 had the minimum (18.84mS cm-1g-1) conductivity value, where G00343 had the maximum value (29.89mS cm-1g-1), which was followed by G00053 and G00221. It was concluded that variation in physio-morphology of the 13 genotypes would be useful in developing short duration high yielding soybean verities through genetic manipulation.

  Soybean, Genotypes, Characterization, Morphology, Seed quality, Yield
  Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur
  00-01-2012
  00-04-2012
  Variety and Species
  Soybean

To characterization, yield performance and analysis of seed quality of exotic 13 dwarf genotypes of soybean.

The experiment was carried out at the experimental field of Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur during January to April 2012. The experimental site is located (24°09 N and 90°26'E) at the center of Madhupur Tract (Brammer, 1971) of Agro ecological Zone (AEZ) 28. Thirteen dwarf soybean genotypes collected from AVRDC, Taiwan which was available at the Dept. of Agronomy of Bangabandhu Sheikh Mujibur Rahman Agricultural University, were included in the present investigation. The genotypes were G0041, G00197, G00166, G00207, G00343, G00204, G00154, G00046, G00221, G00351, G00138, G00053, and G00042. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replications. The whole experimental area was divided into three blocks, representing three replications. Each block was further subdivided into 13 unit plots. The thirteen different genotypes of dwarf soybean represented as treatments of the experiment and were allotted to the 13 unit plots per block. The unit plot size was 1.2 m x 1.0 m accommodating 40 plants in each plot having row and plant spacing of 30 cm and 10 cm, respectively. Fertilizers were applied as urea, triple super phosphate, muriate of potash, gypsum and boric acid as source of N, P,K,S and B @ 50, 150, 120, 80 and 10 kg ha-1, respectively. All intercultural operations were done as soybean production technology. At physiological maturity, dry pods were harvested at different days after sowing (DAS) for different genotypes. The harvesting was started from 90 DAS and continued up to 110 DAS for different genotypes. The crop was harvested upon maturity and pods were sun dried at 9% moisture. Different physio-morphological parameters data were recorded, viz. days to emergence, days to first unifoliate leaf, days to first trifoliate leaf, days to first flowering, days of 50% flowering, branch no. plant-1, stem diameter, days to maturity and plant height. Measurement of SPAD value- The data were recorded from the fully expanded leaf by using SPAD (Soil Plant Analyses Development) meter (MINOLTA SPAD 502) at three days interval from first flowering to physiological maturity. Ten randomly selected plants per genotype were used for measurement SPAD value. The following data were recorded: pod length, pod breadth, number of pods plant-1, number of seeds pod-1, seed color, seed diameter, 100-seed weight, no. of seeds plant-1. Quality attributes of harvested soybean seed- A total of thirty-nine seed samples were collected from the harvested soybean seeds and quality attributes of the harvested soybean seeds (moisture content, germination%, 100-seed weight, seed viability, and seed vigor) were also determined according to ISTA rules. Germination percentage- For germination test, one hundred seeds from each genotype were used and replicated four times. Seeds were placed in 21 cm X 15.5 cm plastic trays containing filter paper soaked with distilled water. Then the plastic trays with seeds were placed in an incubator at 300C till the completion of germination. The germinators were opened every 24 h interval to count germinated seeds and to add water if necessary. Germination was considered to have occurred where a radical length of approximately 2 mm had been reached (Ramin, 1997). Final germination count was made according ISTA, (2006). Data on hard, fresh, rotten and germinated seed were recorded as percentage basis. Germination percentage was calculated by using the following formula: Germination (%) = Nuber of seeds germinated/Number of seeds tested x 100. Seed vigor (electrical conductivity) test- For electrical conductivity test, 10g seeds of each sample were taken in a conical flask containing 100 ml de-ionized water and incubated at 20ºC for 20 hours. After 20 hours, water of the beaker containing seeds was decanted in order to separate the seeds. The electrical conductivity meter was used. Three replications of measurements were made for each sample of seed and expressed on a µS cm-1g-1WIth a conductivity meter (Model-CM-30ET). Seed viability (Tetrazolium) test- The tetrazolium test was used in the determination of seed viability. Soybean seeds were immersed in alcohol for 30 minutes, rinsed with water, immersed in water at 30ºC for 24 hours and had the testa removed. After the preparation procedure, the seed was immersed in tetrazolium solution (0.75%) at 30ºC for 5 hours. Moisture content- Moisture content of seeds was determined by high constant temperature oven method. After fine grinding an amount of 5g of two independently drawn working samples were dried at a temperature of 130- 133ºC for a period of one hour. Percentage of moisture content was recorded to one decimal place (ISTA, 2006) by means of the following formula: % Moisture content = (M2-M3) x M2- M1/100. Where, M1 is the weight in grams of the container and its cover, M2 is the weight in grams of the container, its cover and its contents before drying, M3 is the weight in grams of the container, cover and contents after drying. Statistical Analysis- The data obtained from the experiments on different parameters were analyzed statistically following analysis of variance (ANOVA) technique with the help of computer package, MSTAT. Means were separated using Duncan’s multiple range test at a significance level of 0.05 (Gomez and Gomez, 1984).

  International Journal of Agronomy and Agricultural Research, Vol. 9, No. 5, p. 51-61, 2016
  http://www.innspub.net
Funding Source:
1.   Budget:  
  

Among 13 dwarfs soybean genotypes G00207 produced the maximum grain yield (t ha-1), followed by G00343, G00053, G00138 and G00197due to the highest pods number per plant. Variations were also significant among the genotypes in relation to seed morphological characters, germination behavior and electrical conductivity value. The highest (86.66%) germination was found in G00166. Similarly, the viability was the maximum (91.67%) in G00166.Seed viability had positive relationship with germination, while negative relationship with electrical conductivity value and seed moisture content.

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