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Research Detail

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A. A. Mamun
Seed Marketing Division, Bangladesh Agricultural Development Corporation, Gazipur

U. A. Naher
Soil Science Division, Bangladesh Rice Research Institute, Gazipur, Bangladesh

M.Y. Ali
Agrotechnology Discipline, Khulna University, Khulna, Bangladesh

The experiment was carried out in the Seed Laboratory of Bangladesh Agricultural Development Corporation (BADC), Gazipur during June to July 2014 to investigate the effects of different priming methods on seed germination and the seedling growth of some rice (Oryza sativa L.)varieties. Four rice varieties: 1) Nerica, 2) BRRI dhan51, 3) BRRI dhan41 and 4) BRRI dhan49; and six priming treatments: 1) On-farm Priming, 2) Hardening, 3) Hydro-priming, 4) Osmo-hardening, 5) Vitamin C Priming and 6) Control were used in the experiment. The experiment was laid out in a completely randomized design (CRD). The treatments were replicated three times. The germination percentage and seedling growth parameters differed significantly among the priming treatments. For germination, Nerica variety showed poor performance, whereas the other varieties were found superior. Among the priming treatments, Vitamin C priming and Osmo-hardening were found superior. In case of interaction, germination was increased with Vitamin C priming and Osmo-hardening in Nerica. For seedling growth parameters, particularly vigor index, shoot and root lengths, BRRI dhan41 and BRRI dhan51 were found superior. The priming treatment Vitamin C and Osmo-hardening performed better than others. Seedling vigor index was increased with Vitamin C priming and Osmo-hardening in Nerica variety. Shoot length at 15thday was influenced by hydro-priming in Nerica. Root length at 10thday was significantly increased with hardening in BRRI dhan41. Vitamin C priming and Osmo-hardening could therefore, be used for improving germination and seedling growth of Nerica.

  Priming, Variety, Germination, Seedling growth parameters.
  Bangladesh Agricultural Development Corporation (BADC), Gazipur
  00-06-2014
  00-07-2014
  Seed Technology
  Rice

The present study was therefore, undertaken to examine the efficiency of different priming methods on rice seed germination, seedling growth and to determine the varietal response of rice to seed germination and seedling growth against different priming agents.

The details of materials used and methods employed during the experimental period have been presented in this chapter. 2.1. Design and treatments of the experiment The experiment was conducted with two factors following completely randomized design. Treatments were as follows:Factor A: Priming treatments: a) On-farm Priming (Seeds will be soaked overnight in water before sowing) (Harries et al.,1999) b) Hardening (Alternate soaking of seeds with tap water for 24 hours and dying before sowing in the field) (Basra et al., 2003) c) Hydro-priming (Seeds will be soaked with distilled water for48 hours and dried under shade before sowing in the field) d) Osmo-hardening (Similar to hardening with 1 mole NaCl solution`) e) Vitamin C Priming (Seeds will be soaked with 10 ppm Ascorbic acid solution for 48 hr then dried 24 hours under shade) (Basra etal., 2006)f) Control (Farmer Practice) Factor B: Rice varieties: a) Nerica b) BRRI dhan41 c) BRRI dhan51 d) BRRI dhan49 2.2. Preparation of chemical solution Chemical solutions, NaCl 58.44 gm and Ascorbic acid 0.01 gm of chemicals were added with 1000 ml water. 2.3. Preparation of germination media Wet tissue was used as germination media and Petridis was used as container. The moisture content of the media was maintained at 80% of the field capacity. 2.4. Seed quality parameters The following seed quality parameters were measured: 2.5. Germination Germination test is most commonly used to determine seed viability. The germination test was conducted at 25oC temperature in germination room of Seed Laboratory, Bangladesh Agricultural Development Corporation (BADC), Gazipur. One hundred seeds were taken from each pot and placed in the Petridish. The daily record of germinated seed was taken up to 7th day from set up of the test. 2.9. Seedlings shoot and root length Randomly selected10 normal seedlings from each petridish were taken to record data on shoot and root length. It was recorded at 10 and 15th day.2.10. Seedlings shoot and root dry weight The seedlings for shoot and root length measurements were dried in an electric oven at 72°C for 48 hours. After drying the dry weight of shoot and root were recorded by an electric balance. 2.11. Data analysis The data obtained from different yield components were statistically analyzed to find out the significance of the difference among the treatments. The analysis of variance (ANOVA) was performed by MSTAT-C and means of the parameters were compared using DMRT-Duncan’s new Multiple Range Test (Gomez and Gomez, 1984).

  The Agriculturists 16(1): 34-43 (2018) ISSN 2304-7321 (Online), ISSN 1729-5211 (Print)
  
Funding Source:
1.   Budget:  
  

It may be concluded that there was significant effect of seed priming on different rice varieties. However, the priming effect differed with rice varieties. The variety Nerica showed poor performance; whereas the other varieties were found superior. Vitamin C priming and Osmo-hardening were found superior compared to others priming treatments. Seed germination and seedling growth parameters particularly germination percentages, germination energy, vigor index, shoot and root lengths were increased by the treatments with Vitamin C priming and Osmo-hardening for Nerica. Further investigation may be undertaken to confirm this result and make recommendations on seed priming.

  Journal
  


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