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Research Detail

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MS Islam
Professor, Department of Coastal and Marine Fisheries, Sylhet Agricultural University, Sylhet, Bangladesh

A Hossain
Research Associate, Fisheries Component, KGF Project, Sylhet Agricultural University, Sylhet, Bangladesh

Probiotics effect with dietary supplemental feed on growth and yield of male tilapia (Oreochromis niloticus) in floating cages placed at Dekar haor of Sunamganj for 120 days was assessed. Four treatments viz T1 (Safegut), T2 (Biogen), T3 (Super biotic) and T4 (control) each with three replicates were used in the experiment. Tilapia were stocked in cages at a density of 40 no. m-3 with the average initial weight of 14.23-16.44 g and they were fed with commercial floating feed at a decreasing rate of 10-5% of total biomass thrice daily. Feed was supplemented mixed with probiotics at a rate of 0.5 g kg-1. Higher growth (289.56±14.14g), survival rate (96.91%), yield (10.67±1.7 kg m-3), net profit (BDT 845.25±86.8 m-3) and lower food conversion ratio (1.13) were obtained in T1 than those of other treatments, which were 4-5 times higher than the earthen pond production of tilapia in Bangladesh. The lowest growth, survival, production and higher FCR were found in control treatment where no probiotics was supplied. Therefore, results of the study suggest that probiotics may be applied to supplementary feed to boost up fish production.

  Socioeconomic characteristics, production cost, Return, Factors, problems
  It is situated by the side of Sylhet-Sunamganj high way and close to Sunamganj district town.
  01-07-2018
  29-10-2018
  Variety and Species
  Tilapia

Considering these facts, the present research was undertaken aimed to evaluate the effect of probiotics on growth, survival and yield of monosex tilapia (O. niloticus) in cages.

The study was performed in the Dekar haor one of the most important and largest haor of Bangladesh. Dekar haor lies between latitude 24°46´N to 24°57´N and longitude 91°20´E to 91°31´E. It is situated by the side of SylhetSunamganj high way and closes to Sunamganj district town. The study was conducted for a period of four months from July 1 to October 29, 2018. Twelve nylon net cages (1.5 × 3 × 3 m) were used as a culture system. The frame of the cage was made of a bamboo split. Plastic drums were used as cage float. Net cages were made of plastic or knot-less polyethylene net (mesh 1.0 cm). Cages were hanged with a cage frame. A bamboo-made platform was set up over the cages and all cages were fixed with the poles of the platform. Cages were installed at both sides of the platform for easy feed supply and intensive observation. The open part of each cage was covered with another piece of the net (mesh 7.0-7.5 cm) to avoid escaping fish and predation by bird. This study was one factorial in which probiotics were the only experimental variable as T1 (Safegut), T2 (Biogen), T3 (Super biotic) and T4 (control) each with three replicates. The place selected for setting the cages was cleaned manually and the selected place was limed with agricultural lime (CaCO3) at a rate of 250 kg ha-1. After six days of liming, all cages were stocked with the required quantity of fry of male tilapia (O. niloticus) at a density of 40 no. m-3 with the average initial weight of 14.23-16.44 g. Monosex tilapia fry was purchased from Pirojpur private fish hatchery, Sylhet Sadar, Sylhet and was transported in oxygenated polythene bags from hatchery to experimental area. Before stocking, fry was acclimatized to the cage water for one and half hour period. Initial weight and length of 10% of fishes were recorded before stocking in cages. Stocked fry of tilapia was fed with commercial mega floating feed at a decreasing rate of 10-5% of body weight thrice daily until the previous day of harvest. Proximate compositions as moisture, crude protein, crude lipid, ash, crude fiber, carbohydrate of supplemented feed were respectively 11, 30, 7, 15, 8 and 29%. Probiotics namely Safegut, Biogen and Super biotic were mixed with commercial feed at a rate of 0.5 g kg-1 of feed following the instruction of the manufacturer to assess their role on growth and yield of monosex tilapia. The total daily feed ratio was divided into three equal portions and was applied in the morning between 8.00-9.00 am, at noon 12.00-1.00 pm and in the evening 5.00-6.00 pm. The feeding amount was adjusted every 15-day intervals depending on the bodyweight of stocked tilapia. Net of the cages was cleaned and checked every 7-day intervals. The behavior of tilapia was regularly observed especially after providing feed in the morning and in the evening to determine their conditions as movement, feeding intensity, body colour and diseases. Water quality parameters like surface temperature, transparency, dissolved oxygen (DO) concentration, pH, total alkalinity and ammonia were determined at fortnightly intervals between 9.00-10.00 am at the time of fingerlings sampling. Surface water temperature was measured on the spot using a standard centigrade thermometer. Transparency was recorded using Secchi disc. Dissolved oxygen was determined using a portable DO meter (YSI digital DO meter, Model 58, HANNA Company, America). pH of cage water was recorded using pH meter (HANNA Company, America). Total alkalinity was measured by titrimetric method (APHA, 2000). Ammonia nitrogen was measured using ammonia test kit (Biosol, A.A. Biotech PVT LTD., Fishtech BD Ltd). Fortnightly sampling was done to determine the growth of tilapia fry and to adjust the feed rations. Growth was measured regarding weight (g) with digital balance and length by measuring scale. Tilapia were totally harvested after 120 days of culture. They were caught using a hand scoop net and lifting all cages from the water on the same day. After harvest, all tilapia of cages were counted and weighed individually to determine growth, survival rate and yield. Specific growth rates (SGR), food conversion ratio (FCR) and survival rate (%) were calculated following the equation as cited by Pechsiri and Yakupitiyage (2005). The equations are as follows: Weight gain = Mean final weight – mean initial weight Survival rate (%) = (Number of fish harvested ÷ total number of fish stocked) × 100 SGR (% /day) = {Ln (final body weight) – Ln (initial body weight) × 100}/cultured period. Feed conversion ratio (FCR) = Feed consumed (g dry weight)/live weight gain (g wet weight) of fish Yield of fish = No. of fish caught × (average final weight of fish–average initial weight fish) Economic analyses of tilapia cage farming under different treatments was calculated on the basis of purchasing prices of tilapia fry, feed, fertilizers, lime, transport cost, cage materials and revenue from the sale of harvested tilapia. At the end of the study, all fish were sold at the local market. Tilapia was sold at a rate of Tk. 120.00 kg-1. Net profit and cost-benefit ratio (BCR) were calculated using the following formula: Net profit = Total return – total cost and BCR = Total return ÷ total cost Survival rate, growth and yield variables were analyzed using one-way analysis of variance (ANOVA) to compare the treatments means. If the main effect showed significance, the ANOVA was followed by Duncan’s Multiple Range Test. All ANOVA were tested at a 5% level of significance using SPSS (Statistical Package for Social Science) version 20.

  J. Sylhet Agril. Univ. 5(2):195-202, 2018
  www.jsau.com.bd)
Funding Source:
1.   Budget:  
  

In the present study, the net profit of tilapia farming in cages was the highest (BDT 845.25±86.80 m-3 ) in T1 followed by T2 (BDT 522.35±88.11 m-3 ), T3 (BDT 442.30±82.23 m-3 ) and T4 (BDT 315.27±91.60 m-3 ) (Fig. 4). The benefit-cost ratio (BCR) was also highest in T1 (2.94) followed by T2 (2.26), T3 (2.13) and T4 (1.89) indicating that the highest profit was found from the treatment of safegut probiotics (T1). The reason behind that was the strong positive role of using safegut with supplemented feed owing to this probiotics contains more than four beneficial bacteria and supplemented feed was efficiently utilized. From the above discussion, it may conclude that safegut probiotics are better than the other two types of probiotics. So it can be used with supplementary feed in tilapia cage culture for getting higher yield and profit. It is proven that more beneficial bacteria mean more production and more benefit, which is supported by many researchers (Moriarty, 1996a; El-Haroun et al., 2006 and Verma and Gupta, 2015). In Bangladesh, most of the open water bodies remain fallow round the year due to useful guidelines that have yet not been formulated to manage the floodplain/wetland resources. But these are most valuable. These valuable resources may easily bring under modern cage culture practices in a planned way for increasing fish production and consumption of fish in the country. The findings of the study indicate that safegut probiotics are better than the other two probiotics in the context of growth, production and profit. If fry of monosex tilapia and other inputs can be made available to the farmers in time with a fair price, production of tilapia would be increased, which help to accelerate the farmers’ income as well as national nutritional status

  Journal
  


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