M. A. Rahim*
Department of Horticulture, Bangladesh Agricultural University, Mymensingh
M. J. M. Khatun
Department of Botany, Jahangirnagar University, Saver, Dhaka, Bangladesh
M. Mahfuzur Rahman
Department of Botany, Jahangirnagar University, Saver, Dhaka, Bangladesh
M. M. Anwar
Department of Horticulture, Bangladesh Agricultural University, Mymensingh
M. H. Mirdah
Department of Botany, Jahangirnagar University, Saver, Dhaka, Bangladesh
Roktogota, Haematocarpus validus, Morphology, Nutritional status, Ethnomedicinal fruit
Khagrachari, Chittagong Hill Tracts of Bangladesh.
Development of Host and Medicinal Plants
Medicinal Plants
The study area was phytogeographical region in the district of Khagrachari, Chittagong Hill Tracts of Bangladesh. Freshly collected Roctogota (Haematocarpus validus) from different parts of Khagrachari was used for ethnobotanical study and phyto-chemical analysis. Botanical identification and collection Roctogota (Haematocarpus validus) used as experimental material, was collected from Krisnomohanpara and weekly Hat (Bezza) of Khagrachari sadar, Shotokheda and local market of Ramgorh and Baradom bazaar of Diginala in Khagrachari district through an initial ethno botanical survey. This fruit was collected during April, 2012. The collected fruit was placed in a polythene bag to prevent loss of moisture during transportation to the laboratory. Efforts were made to collect these plants in flowering and fruiting conditions for the correct botanical identification. Detailed ethno botanical knowledge was recorded while collecting voucher specimens. Vouchers of wild edible fruit plant species were collected with knowledgeable local participants and the botanical identification and authentication ware made by the supervisor using the various volumes of the Encyclopedia Flora and Funa of Bangladesh and expert taxonomist in Bangladesh, National Herbarium, Dhaka and checking against identified specimens found in the National Herbarium, Mirpur, Dhaka. The study was conducted at Plant Ecology Laboratory, Department of Botany, Jahangirnagar University, Savar, Dhaka, Bangladesh. Nutrition analysis was done at Postharvest Technologies Division and Soil Science Division of Bangladesh Agriculture Research Institute, Gazipur, Dhaka. Sampling Protocol A multiregional sampling plan (Fig. 1) was employed to collect fruit samples. Every two samples were pooled together to make three analytes (test sample) which were analyzed for their nutrient profile. The ethnic fruit sampling plan is depicted in Figure-1. Specimen collected and examined by: i) M. A. Rahim, from Bandarban on 10-05-2010, ii) M.J.M. Khatun & M. Mahfuzur Rahman, from Khagrachari on 26-4.2011, iii) M.J.M. Khatun, from Rangamati on 21.04.2012. iv) M.J.M. Khatun, Ramgarh, from Khagrachari on 28.05.13. Preparation of fruit sample In laboratory, the freshly collected sample was washed with deionized water to eliminate visible dirt and removed the water quickly with a blotting paper. Then the sample was cut into small pieces, homogenized and accurate amount was weighed as required for different analysis. Three test samples from each fruit were selected for measurement of various parameters. All chemicals and reagents used in analysis were of analytical grade and purchased from Merck (Darmstadt, Germany, BDH, UK). Ascorbic acid and minerals standards, 2,4 di-nitrophenyl hydrazine were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Estimation of PH , Moisture, TSS and Total sugar Weight of Roktogota was estimated by digital weight balance. The pH was determined with a digital pH meter (Ibrahim, 2002) and moisture content was determined by digital moisture analyzer. The Total Soluble Solid (TSS) was determined with a hand refractometer (Gofur et al., 1998). Total sugar was determined by Lane and Eynon method (Ranganna, 1986). Estimation of protein Protein content in the fruit was determined by indirect method estimating total nitrogen in the food. It was calculated by multiplying the total nitrogen using the respective factor as estimated by Micro-Kjeldahl method (AOAC, 1998a). Estimation of fat Powdered fruit was subjected to extraction with mixture of chloroform and methanol (Raghuramulu et al., 2003). Estimation of crude fiber Crude fiber was estimated by gravimetric method as described by Raghuramulu et al. (2003). The dried and fat free fruit sample was treated with boiling sulphuric acid at constant volume, cooled, filtered, washed with hot water, made alkaline, boiled, filtered and washed with water followed by ethanol and ether wash. The residue was then heated in a muffle furnace at 600oC for 3 hours. Crude fiber was finally calculated from the weight difference.
International Journal of Minor Fruits, Medicinal and Aromatic Plants 1(1) 2015:
Journal