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Research Detail

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N.G. Das
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh

K.S. Huque
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh

S.M. Amanullah
Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh

S. Dharmapuri
Food Safety Officer, FAO Food Safety Programme, IPH Building (1st Floor), Mohakhali, Dhaka 1212, Bangladesh

H.P.S. Makkar
Livestock Production Systems Branch, Animal Production and Health Division, FAO, Rome, Italy

The present study was conducted with the objectives of determining the chemical composition and nutritional value of vegetable waste (VW) of households and the marketplace for their suitability as ruminant feed. The crude protein, total digestible nutrients and extent of rumen degradability of dry matter (DM) of VW of households were 140.0 g kg−1, 0.668 and 0.855, respectively; while those of the marketplace was 169.0 g kg−1,0.633 and 0.80, respectively. The levels of chromium and lead in each respectively, were 13.27 and1.53 ng kg−1DM; and 31.01 and 5.71 ng kg−1DM. The total aflatoxins in VW of households was3.08 μg kg−1DM, and undetectable in VW from the marketplace. Considering the chemical composition and safety parameters studied, VW could preliminarily be considered as animal feed. The feeding of processed marketplace VW (VWP) at 275 g kg−1DM of a diet or 0.76% of live weight (LW) to growing bulls, replacing 50% of concentrate mixture as a supplement to a Napier silage diet for a period of 34 days reduced the total DM intake(0.0276 vs 0.0343 LW) without any significant (P> 0.05) changes in DM or protein digestibility. Blood urea levels (19.5 vs 23.67 mg dl−1), and serum creatinine levels (1.37 vs 1.08 mg dl−1)differed significantly(P> 0.05) between the two groups but were within normal physiological ranges. Therefore, it may be concluded that the level of incorporation of VWP would be less than 50% replacement of the concentrate in the diet. Further research is required to determine optimum inclusion levels in ruminant diets.

  Vegetable wastes, Chemical composition, Rumen degradability, Hazardous compounds, Nutrient digestibility
  Animal Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka 1341, Bangladesh
  
  
  Resource Development and Management
  Vegetables

The present study was, therefore, conducted with the objectives of determining the chemical composition, in Sacco degradability and key feed safety parameters of VW, developing a processing system of bulk VW from the marketplace into feed, and determining the impact on the diet intake and digestibility including blood biochemistry on indigenous growing bulls.

1. Collection of VWThe VW output of households was collected from twenty randomly selected households in the residential area of Bangladesh LivestockResearch Institute (BLRI), Savar, Dhaka, Bangladesh for a period of two months (April and May, 2015). All households were appraised of the objectives of the work through group discussion and provided with plastic waste bins for the collection of VW separately from other household wastes. The VW of three vegetable marketplaces of Savar suburban area, Dhaka was collected in separate waste bins daily during the same period of time.2.2. Sample preparation and nutritional evaluation: The collected wastes were processed daily by using a stream of water to remove any dirt, vigorously blended in a power-operated blender (rpm: 1400), dried in the sun, milled into small particles with a locally manufactured feed grinder and then preserved into airtight plastic containers. After the end of the collection, a representative part of all daily milled samples was taken, mixed thoroughly and further ground in a 'Willy Mill’, followed by passing through 1.0 mm screens and then used for laboratory analyses. The vigorous blending of bulk fresh sample, initial grinding for particle size reduction, sub-sampling of daily collection, thorough mixing and then final grinding in the Willymill and sieving through 1.0 mm screen helped to ensure that the sample for chemical analysis was representative. The fresh DM was determined from the fresh samples according to the AOC (2004). Sample DM, organic matter (OM) and crude protein (CP) were determined at the animal nutrition laboratory of BLRI, according to the AOC (2004). A bomb calorimeter (IKAØ Calorimeter System C5003Control, USA) was used for gross energy (GE) estimation. The neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents were determined according to van Soest, Robertson, and Lewis (1991). Total digestible nutrients   (TDN)   were calculated according to Ball et al. (2001). The rumen degradability of each sample at 0, 8, 16,24, 48 and 72 h was determined by using four rumen cannulated bulls of the Cattle Research Farm of BLRI, according to Pskov and McDonald (1979). Duplicate samples of each hour were incubated and an hour of incubation was replicated in four bulls. Thus, a sample of single incubation had eight replications. The VW of household and marketplace were analyzed for heavy metals (lead and chromium), according toBSI (2014); total aflatoxins, according to ISO (2003a); and screened for pesticide residues (12 organochlorine and 52 organopho-sphosphorous compounds) by LC-MS/MS using the QUECHERS method according to BSI (2008)at the National Food Safety Laboratory (NFSL), Institute of Public Health (IPH), Dhaka, Bangladesh.2.3. Production of processed vegetable waste (VWP) from VWThe VW from the marketplace was transported in the evening to a processing center at the Animal Research Station of BLRI. The center had a freshwater supply, locally assembled machines for blending and a concrete floor for sun drying the blended biomass. The VW biomass of marketplace, on the fresh basis, constituted (as fraction) waste cucumber(0.21), followed by 0.180, 0.17, 0.16, 0.09.0, 0.07, 0.06, 0.03, and0.02, respectively of bitter gourd, spotted gourd, brinjal, pumpkin, potato, tomato, ladies finger, and snake gourd during the period of collection. The VW, after collection, was cleaned using a stream of water, and any degraded particles were removed before blending. Depending on the DM content of fresh VW of the marketplace, rice polish was added as an absorbent during blending to facilitate quick drying, ata rate of 200 g kg−1DM of processed VW. At the same time, common salt was added at the rate of 20 g kg−1DM of processed VW to improve the palatability, and thus to help ensure voluntary intake of this feed by the experimental animals. The blended biomass was sundried until the moisture content was reduced to < 120 g kg−1DM of biomass and stored in plastic buckets. A bulk amount of the product, thus produced from VW, hereafter addressed a vegetable waste processed (VWP), was used for feeding growing bulls as one of the major feed ingredients of a conventionally mixed concentrate feed.

  Veterinary and Animal Science 5 (2018) 31–3732
  
Funding Source:
1.   Budget:  
  

Considering the chemical composition, in sacco DM degradability, and feed safety aspects (negligible levels of chromium, lead, total aflatoxin and some pesticide residues), it may be concluded that VW of both households and marketplace could be used as a feed ingredient for cattle. It may also be concluded that dietary inclusion of processed VW feed at the rate of 275 g kg−1 DM (27.5% of diet) or 0.76% of LW of bulls may result in reduced dietary intake (P < 0.01) simultaneously with increased serum creatinine level (P < 0.05) without affecting digestibility. Further research is required to determine the dietary level of processed VW feed in growing bulls to realize optimum production without affecting feed intake and productivity. Year-round quantification of recyclable VW biomass as a feed and development of a sustainable feed manufacturing technology from this may help increase market feed supply by minimizing food-feed competition for cultivable land, and may potentially reduce environmental pollution.

  Journal
  


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