The experiment was carried out at twelve earthen ponds (area: 200 m2 with an average depth of 1.5 m) in Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, Bangladesh. Experimental ponds are prepared the following: dewatering, removing aquatic flora and fauna. All ponds exposed to sunlight with necessary renovation work. Ponds were applied with lime (CaCO3) and fertilized with semi-decomposed cow dung according to Rahman et al.. Fingerlings of each species were collected from local suppliers and cultured for a period of 120 days. Spotted snakeheads were stocked in four treatments: T1 (control), T2, T3, and T4 with 0, 2000, 3000, and 4000 individual ha-1, respectively. Rohu (L. rohita), catla (C. catla), silver carp (H. molitrix), and mrigal (C. cirrhosis) were stocked in each treatment at the rate of 3000, 2000, 2500, and 2000 individual ha-1, respectively. There are three replications for each treatment. During stocking, mean body weight of spotted snakehead (1.50 ±0.07 g), rohu (8.00 ±0.28g), mrigal (8.98 ±0.25g), silver carp (7.15 ±0.12g), and catla (7.35 ±0.10 g) were calculated. The average stocking weight of carps fingerlings was 7.86±0.03 g in all treatments. Fingerlings were fed with a commercial pellet (Naurish Feed. Ltd, Bangladesh: crude protein 35%, moisture 12%, lipid 3%, fiber 10% and ash 14%) at the rate of 5% of their body weight for the starting month, and declined gradually to 3% body weight at the end of the culture period in twice a day (at 09.00 and 16.00). Weighing 10% of the total fish stock was measured to adjust the feeding rate in every month. Ponds were fertilized regularly in seven days intervals with cow-dung at 125 kg ha-1, urea, and triple superphosphate each at a rate of 6.25 kg ha-1 to maintain steady natural food abundance especially plankton and benthos. Water temperature, dissolved oxygen (Dissolved Oxygen Meter, YSI Model 58, Yellow Springs, OH, USA), transparency (Secchi disc), and pH (EC 10 portable pH meter, HACH, Loveland, Co, USA) of all ponds were measured on weekly basis. Nitrogen compounds (NH3-N, NO2-N, and NO3-N) and phosphate phosphorus (PO4-P) were measured bi-weekly by using a HACH kit (DR 5000, HACH). Water samples in plastic bottles (volume of 200 ml) were collected between 09:00 to 10:00 am on each sampling day. For plankton analysis, 10 L of pond water was collected monthly at five randomly selected locations and depths of each pond and filtrated through a 25 µm meshed plankton net. Each filter was then transferred to a measuring cylinder and made up to a standard volume of 50 mL with distilled water and formalin to obtain a 10% buffered formalin solution and preserved in a sealed plastic bottle until examination. Plankton numbers were counted using a Sedgwick-Rafter counting cell (S-R cell) under a binocular microscope (Olympus, M-4000D, Tokyo, Japan) following Stirling. Identification of plankton to the genus level was carried out using the keys from Bellinger. Benthos from each pond bottom was collected in three randomly selected places using a 225 cm2 Ekman dredge every month. Mud samples were washed through a sieve (250 µm mesh size) and macro- benthic organisms were separated and preserved in 10% buffered formalin. The microbenthic animals were then identified and counted following the standard method. All ponds were harvested separately by repeated netting and complete dewatering on day 120. All fish harvested from each pond were counted, measured, and weighed individually. Net production was determined by deducting the stocked biomass from the gross production (harvested biomass). The results were presented as mean ± standard deviation. The data were compared among treatments by One-way analysis of variance (ANOVA) using statistical package for social science (SPSS version11.5) software. Water quality parameters, plankton, and benthos data were compared in a repeated measure analysis; however one-way ANOVA was also performed. When the mean difference was significant (P< 0.05), Tukey’s test was used to compare the mean among all the treatments.