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Research Detail

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Sajibul Hasan
Lecturer
Department of Preclinical Courses, Faculty of Veterinary and Animal Sciences, Gono University, Savar, Dhaka, Bangladesh

Mohammad Alam Miah
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensing-2202, Bangladesh

Md. Kamrul Islam
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensing-2202, Bangladesh

The present study was carried out to investigate the haemato-biochemical profile during different stages of lactation in local Sahiwal crossbreed dairy cows at Savar area of Dhaka district of Bangladesh. Twenty-four local Sahiwal crossbreed cows were selected randomly from different small farms and divided into three groups: early-stage lactation (1-3 months), mid-stage lactation (4-6 months) and late-stage lactation (7-9 months). Blood samples were collected during three stages of lactation. There were no statistically significant (p<0.05) variations observed in hematological parameters amongst the three-stage of lactation. Although the packed cell volume (PCV), hemoglobin (Hb) concentration and RBC count were found lowest in early stage of lactation than the other groups. Total leucocyte count (TLC), platelet count (PLT), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) were recorded within normal limits. Similarly, biochemical parameters were observed statistically non-significant amongst the groups studied. The glucose level was recorded lowest in early stage of lactation; whereas, the protein and creatinine concentrations were slightly higher in this stage. Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) were not varied amongst the three groups of cows. Data generated during the current study may be useful as reference values for the scientific community.
 

  Haemato-biochemical profile, Lactation stage, Sahiwal dairy cows
  Savar area of Dhaka district of Bangladesh
  
  
  Animal Health and Management
  Cow

To investigate the variations in haemato-biochemical profile during different stages of lactation in local Sahiwal crossbreed dairy cows of Bangladesh.

Experimental design:

The experiment on hemato-biochemical profile during different stages of lactation in local Sahiwal crossbreed dairy cows were carried out in different small dairy farms at Nolam area of Savar, Dhaka. Twenty four local Sahiwal crossbreed cows were selected randomly from different small farms and divided into three groups, depending on the day postpartum of sampling, each group consists of eight dairy cows: group I: 1-3 months after calving (early stage of lactation), group II: 4-6 months after calving (mid stage of lactation), group III: 7-9 months after calving (late stage of lactation). They were reared in same environmental management and also provide normal lactating ration.

Collection of blood samples:
Blood samples were collected aseptically from each animal of all the three groups by jugular vein puncture into collection tubes containing anticoagulants or without anticoagulants for hematological and biochemical analysis, respectively.

Hematological analysis:
Collected blood samples were analyzed for different hematological parameters including total erythrocyte count (TEC), total leucocyte count (TLC), platelet count (PLT), hemoglobin concentration (Hb), packed cell volume (PCV), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) using automated hematology analyzer (Cell-Dyn 3700, Abbott Diagnostics, USA).

Biochemical analysis:
The blood samples were allowed to clot and after clotting, the samples were centrifuged properly, then clear blood serum were separated into clean test tube. Then the serum samples were analyzed for different biochemical parameters including glucose, total protein, creatinine, blood urea nitrogen (BUN), alanine aminotransferase (ALT), asparate aminotransferase (AST) and alkaline phosphatase (ALP) by employing dry chemistry discs/cartridges in Piccolo Xpress Chemistry Analyzer (Abaxis, USA).

Data analysis:
The results were statistically analyzed using one-way ANOVA as per the method of Snedecor and Cochran (1994). P < 0.05 was considered to be statistically significant.

  Asian J. Med. Biol. Res. 2021, 7 (1), 1-5; doi: 10.3329/ajmbr.v7i1.53302; ISSN 2411-4472 (Print) 2412-5571 (Online)
  www.ebupress.com/journal/ajmbr
Funding Source:
  

The present study investigated the hemato-biochemical profile during different stages of lactation in local Sahiwal crossbreed dairy cows at Savar area of Dhaka district of Bangladesh. There is no significant variation found among the hemato-biochemical profile during different stages of lactation. Nonetheless, data generated during the current study may be useful tools for the scientific community as this is the first study of its kind in case of Sahiwal cow in Bangladesh. Further, blood profile has traditionally been used to assess the metabolic health status of the animals; hence the present investigation may also be helpful in this regard. In addition, this study may also assist the nutritionists to formulate ration for optimum productivity of the dairy cow since blood-biochemical analyses are being widely considered to identify dietary causes of diseases leading to low productivity.

  Journal
  


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