Rajshahi district falls within the Rajshahi division of Bangladesh. It is bordered by Naogaon district on the north, Kushtia district on the south, Natore district on the east and Nawabganj district on the west. Bangladesh has 64 districts; the predominantly rural populations of these districts rely on traditional medicinal practitioners for treatment of various ailments. These practitioners are experts in the knowledge of medicinal plants and their properties. Each has his own formulations and dosages based on the practitioner’s individual experience, and this information is passed from generation to generation and not usually shared with other practitioners. We are conducting a district-wise ethnobotanical survey of medicinal plants used to treat various ailments, which varies considerably from district to district. Interviews were conducted with a number of traditional medicinal practitioners in Rajshahi district using a semi-structured questionnaire. Detailed information was collected as to plants, plant parts or combination of plants used, formulation of medicines, dosages as well as ailments for which the plants are used. Plant specimens as pointed out by the traditional medicinal practitioners were collected and brought to Bangladesh National Herbarium for complete identification. Detailed information was obtained on 32 plant species belonging to 24 families. These plant species (ailments for which they are used given within parentheses) include Achyranthes aspera (jaundice), Amaranthus spinosus (dysentery, rabies), Vinca rosea (diabetes), Typhonium trilobatum (body ache), Calotropis procera (edema in pregnant women), Heliotropium indicum(antidote to poisoning, bone fracture), Cannabis sativa (insomnia, coughs, sexual stimulant), Terminalia bellerica (loss of appetite, acidity, hair loss, intestinal worms), Terminalia chebula (loss of appetite, acidity), Commelina bengalensis (poisoning), Coccinia cordifolia (diarrhea, blood dysentery, dizziness from sunstroke), Emblica of icinalis(digestive aid, acidity), Swertia chirata (fever), Ocimum sanctum (malaria, coughs, gonorrhea), Clerodendrum viscosum (stomach pain, acidity, fever), Clitoria ternatea (urinary problems), Cassia alata (lowsperm count, skin disorders), Cassia tora (flatulence, abdominal pain from intestinal worms, blood poisoning, eczema), Erythrina variegata (eye itch), Abutilon indicum (lack of breast development in fema les), Sida cordifolia (nerve weakness, low sperm count, cloudy urine), Azadirachta indica (gall bladder and pancreatic disorders, chicken pox), Moringa oleifera (cancer), Nyctanthes arbor-tristis (fever), Piper longum (hypertension), Cynodon dactylon (bleeding from mouth, stomach disorders, wounds, blood with vomit, irregular menstruation), Aegle marmelos (indigestion), Scoparia dulcis (oral lesions, frequent urination in children), Datura metel (swellings, pain, abscess), Nicotiana plumbaginifolia (piles), Abroma augusta (weakness, hypertension) and Centella asiatica (blood disorders, fever).