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Effect of Genotype and Lactation on Milk Urea Nitrogen, Blood Urea Nitrogen and Milk Composition of Dairy Cows

M. S. K. Sarker
Animal Production Research Division, BLRI, Savar, Dhaka-1314.

M. A. Islam
Animal Production Research Division, BLRI, Savar, Dhaka-1314.

K. S. Huque
Animal Production Research Division, BLRI, Savar, Dhaka-1314.

N. R. Sarker
Animal Production Research Division, BLRI, Savar, Dhaka-1314.

M. M. Hossain
Department of Animal Science, BAU, Mymensingh-2202

A. A. Bhuiyan
Poultry Production Research Division, BLRI,l Savar, Dhaka-1314

Abstract/ Executive Summary:

This study was conducted to evaluate milk urea nitrogen (MUN) of cows considering variations in dietary nutrition, genetic quality and lactation yield. A total of forty dairy cows consisting 20 native and 20 crossbred milking cows were selected from Sonaimuri, Noakhali district in winter season to know the daily feed availability to cows. Feed milk and blood samples were collected and analyzed. The Dry Matter Intake (DMI) intakes of the local and crossbred cows were 2.58 and 2.74 g/100kg live weight respectively. Metabolizable energy (ME) and protein intake showed significantly higher values in crossbred cow (85 MJ/day and 815 g/day) compared to local cow (40 MJ/day and 395 g/day) in winter season (P>0.05). Live weight, BCS and milk yield were varied significantly between genotypes including MUN value although no statistical differences in BUN value were recorded. Strong correlation between lactose and protein percentage was observed in both the lactations in local cows. Milk minerals are negatively correlated with protein, Solids-not-fat (SnF) and minerals in first lactation where as moderate to strong relation was observed in second lactation with those parameters. Milk constituents didn’t show any difference between local & crossbred cows. There was a strong correlation in milk protein with lactose and SnF were observed in both local and crossbred cows. Blood urea nitrogen (BUN) value showed a moderate correlation between milk yields of local cows. Considering the parameters, it can be noted that genotype and lactation have no effect on BUN value but MUN value showed significant difference with local and crossbred cows in dry season.

Keywords: Milk, Milk composition of dairy cows, Blood urea nitrogen, Urea nitrogen, Lactation on milk, Cows

Location: Sonaimuri Upazila, (Noakhali district), Bangladesh

Start Date: 01-07-2010

End Date: 30-06-2011

Program Area: Food Safety and Security

Commodity: Milk

Objectives:

This study was undertaken to determine the existing status of MUN, on-farm biological variables that affect it and to know the on-farm plane of nutrition of cows at Sonaimuri, Noakhali in winter season.

Methodology:

Ten cows, each of native and crossbred origins differing in lactation yield were selected in Sonaimuri (Noakhali), in winter season of the country. The data on feed and feeding systems of the two genotypes of cows were collected through a survey schedule and reprehensive samples of feeds, fed to the cows were collected and analyzed for nutrient composition using standard methods to determine the status of nutrition. Daily milk yield was recorded and samples of morning and afternoon milk were collected and analyzed for fat protein, lactose, SnFG and minerals in Dairy Science Lab. of APR, BLRI using a milk analyzed. Blood samples were collected directly from the jugular vein and serum was separated. Milk and blood serum were analyzed following a newly developed High Performance Liquid Chromatography (HPLC) based method of Animal Production Research Division Lab. (Knauer, Berlin, Germany) for MUN and BUN content. HPLC- binary gradient or isocratic system consisting a PHLC-Pump K-501 solvent delivery system, a manual injector, a K-2501 spectrophotometric UV detector all from Knauer, a 250 mm x 4mm NH2 column, 95% acetonitrile in de-ionized water @ 1.0 ml/min was the mobile phase and detection was done at 195 nm. Only for BUN values, the results were cross checked with an analytical kit. 0, 0.5, 1.0, 2.0, 4.0, 6.0 and 10.0 mg/dl urea standard solution was prepared in acetonitrile. 500µl milk sample & 500 µl metaphosphoric acid solution (1.12%) was taken in a eppendrof tube, the mixture was vortexed & centrifuged at 3000 rpm at 4°C for 20 min to precipitate protein. 100µl supernatant was taken to another eppendrof tube and 900µl acetonitrile was added, mixed well & centrifuged at 1000 rpm at 4°C for 10 min to precipitate. Supernatant was filtered with syringe filter & 80 µl supernatant was injected.

Source of Information: Proceedings of Annual Research Review Workshop 2010-2011. Bangladesh Livestock Research Institute, Savar, Dhaka-1341

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that genotype and lactation have no effect on BUN value but MUN value showed significant difference with local and crossbred cows in dry season. there was a strong correlation in milk protein with lactose and SnF were observed in both local and crossbred cows.

Knowledge Management: Report/Proceedings