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In Vitro Regeneration of Potato Under Salt Stress

Dr. Mohammad Zakaria
Associate Professor
Department of Horticulture Bangabanbhu Sheikh Mujibur Rahman Agricultural University (BSMRAU) Gazipur-1706.

Md. Sanaullah Biswas
Assistant Professor
Department of Horticulture Bangabanbhu Sheikh Mujibur Rahman Agricultural University (BSMRAU) Gazipur-1706.

Abstract/ Executive Summary:

In vitro screening of potato cultivars (Diamant, Felsina and Challisha) were investigated with five levels of NaCI (0, 30, 60, 90 and 120 mM). Significant differences were noticed among the cultivars in response to different levels of NaCl. Plant growth and root development gradually depressed with increased concentration of NaCl in MS media. All three cultivars survived well with exhibiting different growth status up to 60 mM NaCl but they performed very less at 120 mM concentration of NaCl. Variety Challisha performed better regarding shoot length, root length, number of nodes per plantlet and fresh weight per plant upto 90 mM concentration of NaCl. The MS media containing 30 mM NaCl had no much effect on the growth traits of in vitro plantlets of potato.

Keywords: Potato, Regeneration, In-Vitro, Salt stress

Location: Department of Horticulture, (BSMRAU), Gazipur-1706

Start Date: 01-07-2009

End Date: 31-06-2010

Program Area: Variety and Species

Commodity: Potato

Objectives:

1. To develop an efficient screening technique of salinity resistant potato lines and 2. To evaluate salt-tolerant cultivars effective for future use under salinity conditions.

Methodology:

The experiment was carried out at the Tissue culture laboratory of the Department of Horticulture, BSMRAU, Gazipur during July 2009 to June 2010. Sprouted healthy tubers of variety Diamant, Felsina and Challisha were selected as the primary source of explants. The experiment contained three potato cultivars (V1-Challisha, V2-Diamant and V3-Felsina) and five concentrations of NaCl salt (C0- 0 mM, C1- 30 mM, C2- 60 mM, C3- 90 mM and C4- 120mM) which in combination made 15 treatment combinations. In vitro plantlets were regenerated through sprout culture. About four weeks old in vitro plantlets of Diamant, Felsina and Challisha were prepared with shoot apex by discarding roots. Single nodes (1 cm) were separated and cultured in MS medium (Murashige and Skoog, 1962) modified as per treatment combination. The medium contained 3% sucrose and solidified with 0.8% agar. All the cultures were incubated at 25±2ºC with 16/8 h D/N cycle at cool white fluorescent light. The pH of the medium was adjusted to 5.8 and autoclaved at 121ºC for 20 min. Three nodal segments were transferred in each culture vessel and three replications of each treatment were employed. The experiment ended on 4 weeks and the growth response were noted on root length, shoot length, number of node, number of leaf, number of root and fresh weight. The recorded data were analyzed statistically with the help of computer following MSTAT-C program. Mean separation were done by DMRT test.

Source of Information: Research Report, Personal contact with Principal Investigator

Article Link (Online Journal):

Funding Source:

1.	Budget: 50,000.00
2.	Budget: 50,000.00

Total Budget (Tk.) : 50,000.00

Achievement/Findings:

In vitro screening of potato cultivars Diamant, Felsina and Challisha were investigated with five levels of NaCI. Significant differences were noticed among the cultivars in response to different levels of NaCl. Plant growth and root development gradually depressed with increased concentration of NaCl in MS media. All three cultivars survived well with exhibiting different growth status up to 60 mM NaCl but they performed very less at 120 mM concentration of NaCl. Variety Challisha performed better regarding shoot length, root length, number of nodes per plantlet and fresh weight per plant upto 90 mM concentration of NaCl. The MS media containing 30 mM NaCl had no much effect on the growth traits of in vitro plantlets of potato. It is recommended to take more potato cultivars and wild variety under micro propagation scheme and consequently it can be used as a tool for the selection of salt tolerant line for field transplantation and also for designing breeding programme.

Knowledge Management: Report/Proceedings