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Characterization of Sugarcane Using Molecular Markers

Dr. Md. Amzad Hossain
Chief Scientific Officer
Head, Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Nadira Islam
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

B P Podder
Chief Scientific Officer
Breeding Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Dr. Md. Abdus Samad Miah
Director General
BSRI, Ishurdi, Pabna

Md. Samsul Alam
Professor
Department of Fisheries Biology, Bangladesh Agricultural University, Mymensingh- 2200

Abstract/ Executive Summary:

This experiment was conducted to establish varietal identification and determine the variation among the sugarcane varieties/clones through DNA Fingerprinting at BSRI and BAU Lab during the cropping season 2009-2010. This investigation was conducted using ten sugarcane varieties such as Isd 16, Isd 19, Isd 20, Isd 26, Isd 28, Isd 29, Isd 30, Isd 31, Isd 33 and Isd 35.An efficient and easy method was adopted for isolation of high quality DNA from meristem cylinder without liquid nitrogen. The amount of recovered DNA was enough for Polymerase Chain Reaction (PCR) amplification and marker studies such as SSR markers. DNA fingerprinting was performed using SSR Primer SMC 319 CG Electrophorograme of ten varieties using one primer. The results of the present investigation revealed that SSR primer SMC 319 CG was able to identify the ten sugarcane varieties.

Keywords: Sugarcane Molecular Markers.

Location: BSRI and BAU Lab

Start Date: 01-07-2005

End Date:

Program Area: Variety and Species

Commodity: Sugarcane

Objectives:

a. To determine the variation among the sugarcane varieties and active germplasm collection of BSRI using molecular markers. b. To establish varietal identification of sugarcane through DNA Fingerprinting. c. To develop Marker Assisted Selection (MAS) method for sugarcane.

Methodology:

a. The BSRI-bred varieties, as well as active germplasm collection preferably flowering germplasm those collected from home and abroad were used as experimental materials. b. DNA extraction and purification were followed by the modified mini prep CTAB method. Three individual DNA samples from a cultivar or germplasm in equal volume and concentration were pooled to form the bulked sample. c. RAPDs and SSRs primers were used in PCR amplification. d. Amplified DNA were run in Agarose Gel and Polyacrylamide Gel by electrophoresis to separate allele specific bands. e. Electrophoresis Gel were documented for Fingerprinting using Gel Documentation System, UV-Transilluminator and Digital Camera as and where required. f. Documented Electrophoreses Gel were scored on the basis of the presence or absence of the allele specific bands. Data were analysed to determine the relationships among all cultivars and/or germplasm collection and cluster analysis were carried out using computer software.

Source of Information: Annual Research Programme 2009-2010 & Annual Report 2010-2011

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 12,50,200.00

Total Budget (Tk.) : 12,50,200.00

Achievement/Findings:

DNA fingerprinting of ten sugarcane varieties such as Isd 16, Isd 19, Isd 20, Isd 26, Isd 28, Isd 29, Isd 30, Isd 31, Isd 33, Isd 35 using one SSR primer SMC 319 CG were performed.

Knowledge Management: Report/Proceedings