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Characterization and Ex-situ Conservation of an Endangered Carp, Kalibaus Labeo Calbasu

Md. Nahiduzzaman
PhD Fellow
Department of Fisheries Biology and Genetics, BAU, Mymensingh-2202

Abstract/ Executive Summary:

For the morphometric and truss network measurements, plotting DFs revealed high isolation of the stocks. The dendrogram based on morphometric and truss distance data placed the Jamuna and Hatchery in one cluster. The Halda and the Padma constructed one cluster with one sub-cluster with Haor population. Microsatellite DNA marker analysis was also carried out to assess the population genetic structure of L. calbasu, collected from four wild and a Hatchery sources. Twenty five (25) primers developed from rohu (Labeo rohita), catla (Gibelion catla) and carpio (Cyprinus carpio) were tested for PCR amplification of which nine (9) primers were amplified successfully. Out of 9 (nine) loci, four microsatellite markers (Lr10, Lr21, Lr24 and Lr26) developed from rohu and one microsatellite marker (CcatG1) developed from catla were analyzed to test the genetic variability of the kalibaus stocks. The maximum number of alleles observed in loci Lr10, Lr21, Lr24 and Lr26 and CcatG1 were 4, 6, 4, 5 and 10, respectively. The loci were found to be polymorphic

Keywords: Genetic characterization, Ex-situ, Carp

Location: Bangladesh

Start Date:

End Date:

Program Area: Conservation and Biodiversity

Commodity: Carp fish

Objectives:

The study was aimed at the phenotypic and genetic characterization of an endangered carp, kalibaus Labeo calbasu and development of sperm cryopreservation protocol for ex-situ conservation.

Methodology:

This study was aimed at the phenotypic and genetic characterization of an endangered carp, kalibaus Labeo calbasu and development of sperm cryopreservation protocol for ex-situ conservation. Landmark-based morphometrics and meristic counts were applied to evaluate the population status of L. calbasu collected from four wild (the Jamuna, the Padma, the Halda and the Haor) and one hatchery sources. Six (standard length, head length, eye length, peduncle length, pre-orbital length and maxillary barbel length) of 12 morphometric measurements, two (pectoral fin rays and scales above the lateral line) of 9 meristic counts, and all (except- 4 to 5 and 5 to 7) of 22 truss network measurements revealed a highly significant inter stock variation (P<0.01, P<0.001) between all samples. Plotting Discriminant function DF1 and DF2 showed a clear differentiation between stocks for both morphometric and landmark measurements. For both morphometric and landmark measurements, the first and second DF accounted 73% and 15.7% of among group variability and together they explained 88.7% of the total group variability.

Source of Information: PhD thesis of Md. Nahiduzzaman,Roll No.1/2006-07, Regn. No./ Session: 15599 (1987-88),Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Detailed phenotypic and genetic characterization has been done to select the superior Labeo calbasu stock for ex-situ conservation. For the morphometric and truss network measurements, plotting DFs (Duscriminant Function) revealed high isolation of the stocks. The average number of allele (5.6) and observed average heterozygosity (Ho) (0.770) were the highest in the Jamuna population. Sperm of L. calbasu diluted in Alsever’s solution at 1:10 dilution ratio stored in refrigerator (4^oC) produced desirable results and could be cryopreserved using Alsever’s solution with 10% DMSO (5-10 min equilibration) and 10% methanol (20-25 min equilibration) with a cooling rate of 10^oC/min from 5 to-80^oC.

Knowledge Management: Thesis