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Identification of Phomopsis Resistance Gene in Eggplant

Mr. Md. Ibrahim Khalil
PhD Fellow
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh.

Abstract/ Executive Summary:

Amplification with five decamer primers generated 69.0, 66.1 and 65.2% polymorphic bands for F4, F5 and F6 lines, respectively. The dendogram constructed from Nei’s genetic distance produced parent cultivars merged into one subgroup with resistant cultivar BAU Begun-1 formed a separate sub-group in all the generations studied. The lines through F4 to F6 grouped into sub-cluster together, that indicates some common trait was being shared by all of these advanced lines of both the crosses. This might be the resistance trait inherited from BAU Begun-1 as supported by other characters like fruit color and fruit shape also inherited from the parents. A PCR-based molecular cloning technique was used to identify Nucleotide Binding Site-Leucine Rich Repeat (NBS-LRR) type resistance gene sequence in eggplant. Primers were designed based on previously used in sugar beet and the resultant amplicons were cloned. Nine clones were sequenced and found to be about 243 nucleotides in length. Most of the sequences have similarity with TIR and non-TIR types of NBS-LRR gene of Solanaceous crops. These sequences also have similarity with I2 gene sequence which is a non-TIR type resistance gene in tomato- that confers resistances to the tomato wilt pathogen. Another experiment was conducted to determine if an osmotin gene in eggplants correlates with phomopsis resistance. Primers were designed against homologous regions in osmotins, and made to be most similar to sequences in Solanaceous crops. These primers were used to PCR amplify and clone ~700 bp sequence from both the resistant BAU Begun-1 and the susceptible Laffa S eggplant cultivars. Analysis of the sequences revealed six different types osmotin like sequence (Genes or alleles) for the phomopsis susceptible eggplant cultivar Laffa S and resistant cultivar BAU Begun-1 which encodes some conserved osmotin amino acids including 16 of the characteristic Cysteine residues and five acidic cleft amino acid residues. This means that there were at least 3 different osmotin-like genes in eggplants. Four types of sequences were amplified from both the cultivars. One type of sequence (C) was found only in BAU Begun-1 and another (E) only in Laffa. PCR products were successfully digested with restriction enzymes in presence of PCR buffer without any purification and addition of any reagents to find out the allele or gene responsible for resistance in eggplant. Further analysis of the C and E type sequence uncommon in the resistant and susceptible eggplant cultivars would reveal the presence or absence of any other resistance trait in BAU Begun-1.

Keywords: Identification, Eggplant, Phomopsis resistance gene

Location: Mymensingh

Start Date: 00-00-2006

End Date: 00-00-2009

Program Area: Variety and Species

Commodity: Brinjal

Objectives:

The experiment was conducted for identification of Phomopsis Resistance Gene in Eggplant

Methodology:

The F₄, F₅ and F₆ lines of eggplant derived from the crosses of Dohazari G x BAU Begun-1 and Laffa S x BAU Begun-1 were evaluated for resistance to phomopsis blight and fruit rot. The three parents and seven lines were inoculated with Phomopsis vexans under field conditions in Rabi seasons of 2006-07, 2007-08 and 2008-09. The inoculated plants exhibited differential disease reactions. Among the parents, BAU Begun-1 was resistant whereas Dohazari G and Laffa S were susceptible to P. vexans. All the lines of three generations showed resistant reaction to the disease. Significant differences were observed among the phenotypes in all the yield components. High genotypic and phenotypic coefficient of variation, heritability and per cent genetic advance were recorded for number of fruits per plant, number of secondary branch per plant, fruit length and fruit width in all the generations. Significant positive correlation was observed between yield contributing characters. Random Amplified Polymorphic DNA (RAPD) technique was used for assessing genetic variation and relationship among three parent cultivars and their advanced lines of eggplant.

Comments: Are the coloured sentences part of methodology?

Source of Information: PhD thesis of Mr. Md. Ibrahim Khalil ,Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh. Year 2012

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The advanced lines of eggplant were found to be resistant to phomopsis blight and fruit rot. Osmotin-like gene sequence was identified from eggplant which is responsible for phomopsis resistance.

Knowledge Management: Thesis