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Study of Biotypes of Macrophomina Phaseolina Under Controlled Condition for Resistant Materials

K.Sultana
Principal Scientific Officer
Plant Pathology Department,Pest Management Division, BJRI, Manik Mia Avenue,Dhaka-1207.

S.M.A. Haque
SO
Plant Pathology Department,Pest Management Division, BJRI, Manik Mia Avenue,Dhaka-1207.

Q.M. Mosaddeque Hossen
SO
Plant Pathology Department,Pest Management Division, BJRI, Manik Mia Avenue,Dhaka-1207.

Abstract/ Executive Summary:

The experiment was conducted in Green house, Central station, Dhaka to find out the sources for resistant materials against Macrophomina phaseolina. during 2005 jute seasons. Eleven deshi, 9 tossa and 8 kenaf germplasms were screened at greenhouse against two strains of Mcrophomina phaseolina (MS-6, MS-9). Out of twenty eight germplasm, only four (4620, KE-3, SDL-3 and American kenal) had resistant reaction.

Keywords: Resistant, Jute,Macrophomina phaseolina, Biotypes

Location: Green house, BJRI

Start Date: 30-03-2005

End Date: 30-11-2005

Program Area: Pest Management

Commodity: Jute

Objectives:

1. To find out the sources for resistant materials against Macrophomina phaseolina

2.To help the breeders to develop the resistant varieties against Macrophomina phaseolina.

Methodology:

A total of 28 germplasm accessions and one variety of Corchorus sp. kenaf and mesta was sown in the pots following CRD with three replications. Three plants (30-40 days old) of each treatment was inoculated on 3 spots of each plant with 48 hours old culture of each race grown in PDA medium. Circular block ( 2mm diameter) was cut with a block cutter. One block was placed on each spot on stem after pricking with a needle. The pots were kept inside the humid chamber in the green house for 48hours where 90% humidity ( about 90% & temperature 30^oC was maintained. After 48 hours pots were taken out of the chamber. Records were kept: a) Necrotic lesions was measured in mm after 7-10 days of inoculation. b) Disease reaction was assessed on the basis of lesion size & grouped as resistant (R), moderately resistant (MR ), Susceptible (S ) and Highly Susceptible (HS ). c) Seeds from resistant and moderately resistant treatments was collected.

Source of Information: Annual Report,2005, BJRI

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) : Five thousand taka only

Achievement/Findings:

Eleven deshi, 9 tossa and 8 kenaf germplasms were screened at greenhouse against two strains of Mcrophomina phaseolina (MS-6, MS-9). Out of twenty eight germplasm, only four (4620, KE-3, SDL-3 and American kenal) had resistant reaction.

Knowledge Management: Report/Proceedings