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Investigation for Resistant Materials of Macrophomina Phaseolina Under Controlled Condition

K.Sultana
CSO
plant pathology Department,Pest Management Division, BJRI, Manik Mia Avenue,Dhaka-1207

Abstract/ Executive Summary:

The experiment was conducted in green house, BJRI to find out the resistant sources against Macrophomina phaseolina.Twenty-six materials of capsularis and olitorius were screened against two virulent races (biotypes) of M. phaseolina in greenhouse. Three accessions # 2407, 2419, 2496 showed tolerant reaction against two biotypes (MS-6 and MS-9) of M. phaseolina. Six accessions # 2137, 2138,2139,2141, BJC-2142 and 2499 showed susceptible reaction. Rest of the accessions showed moderately resistant reaction.

Keywords: Investigation, Resistant, Jute, Macrophomina phaseolina

Location: Cntral station,Dhaka

Start Date: 30-03-2007

End Date: 30-11-2007

Program Area: Pest Management

Commodity: Jute

Objectives:

1. To find out the sources for resistant materials against Macrophomina phaseolina

2.To help the breeders to develop the resistant varieties against Macrophomina phaseolina.

Methodology:

A total of 26 materials of Corchorus sp. was sown in the pots following CRD with three replications. Three plants (30-40 days old) of each treatment was inoculated on 3 spots of each plant with 48 hours old culture of each race grown in PDA medium. CIrcular block ( 2mm diameter) was cut with a block cutter. One block was placed on each spot on stem after pricking with a needle. The pots were kept inside the humid chamber in the green house for 48hours where 90% humidity ( about 90% & temperature 30oC was maintained. After 48 hours pots were taken out of the chamber. Records were kept: a) Necrotic lesions was measured in mm after 7-10 days of inoculation. b) Disease reaction was assessed on the basis of lesion size & grouped as resistant (R), moderately resistant (MR ), Susceptible (S ) and Highly Susceptible (HS ). c) Seeds from resistant and moderately resistant treatments was collected.

Source of Information: Annual Report, 2007, BJRI

Article Link (Online Journal):

Funding Source:

1. Government

Budget: Five thousands taka only

Total Budget (Tk.) : Five thousands taka only

Achievement/Findings:

Twenty-six materials of capsularis and olitorius were screened against two virulent races (biotypes) of M. phaseolina in greenhouse. Three accessions # 2407, 2419, 2496 showed tolerant reaction against two biotypes (MS-6 and MS-9) of M. phaseolina. Six accessions # 2137, 2138,2139,2141, BJC-2142 and 2499 showed susceptible reaction. Rest of the accessions showed moderately resistant reaction.

Knowledge Management: Report/Proceedings