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Preparation of Appropriate Media for Bacterial Growth and Their Effect on Jute Retting

M. Shamsul Haque
Principal Scientific Officer
Fibre Quality Improvement Division, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207.

M. Abdul Halim
Scientific Office
Fibre Quality Improvement Division, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207.

M. S. I. Talukder
Scientific Officer
Fibre Quality Improvement Division, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207.

Suraiya Khandker
Chief Scientific Officer (cc)
Fibre Quality Improvement Division, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207.

M. Younus Ali
Scientific Officer
Fibre Quality Improvement Division, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207.

M. Kamruzzaman
Scientific Officer
Fibre Quality Improvement Division,Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207.

Abstract/ Executive Summary:

Culture media are essential for proper growth of all bacteria (microbes) having good retting activity for isolate and identify of different microbes. All types of media are not equally suitable for the growth of all bacteria having retting activity. During the screening or isolation of promising microbes, three kinds of culture media viz. nutrient agar media, peptone yeast extract and beef liver-jute bark (BLJB) medium were used. The experiment was undertaken to accelerate their retting activity and was conducted at Manikgonj station to select appropriate culture media for the growth of promising bacteria having the retting capability and to minimize the retting time as well as to improve the quality of fibre in the period of 2005 to 2007. The isolated bacteria viz. Micrococcus sp., Bacillus sp, and Pseudomonas sp. grew well in beef liver-jute bark (BLJB) medium and nutrient agar (NA). In 2005, out of which ten strains, five strains grew well in Nutrient Agar media of which one strain (Micrococcus sp.1) was found to be the best retter which retted time was 6 days along with A grade fibre and other four strains (Micrococcus sp.4, Micrococcus sp.5, Bacillus sp.2 and Bacillus sp.3) were moderate. On the other hand, six strains grew well in Starch Agar media of which one strain (Clostridium sp.5) was found to be the best retter which retted time was 6 days along with A grade fibre and other three strains (Clostridium sp.1, Clostridium sp.2 and Clostridium sp.6) were moderate retters and rest of two were non retters. In 2006, out of the three bacterial strains, two strains (Bacillus sp. and Pseudomonas sp.) retted green jute ribbons at 10 and 14 days respectively grown in nutrient agar media but Bacillus sp. and Micrococcus sp. grown in peptone yeast extract medium and retted the jute ribbons at 13 days and 15 days respectively. The degrees of retting were ++++ and ++ for the Bacillus sp., Pseudomonas sp. Bacillus sp. and Micrococcus sp. respectively. In 2007, out of the five bacterial isolates viz. Micrococcus sp., Clostridium sp. and Bacillus sp. were grown on beef liver-jute bark (BLJB) media and retted green jute stem at 10-12 days respectively where Pseudomonas sp. and Bacillus sp, grew in nutrient agar media and retted green jute stem at 15 and 17 days respectively. The degrees of retting were +++ and ++ for the Micrococcus sp & Clostridium sp. and Bacillus sp. & Pseudomonas sp. respectively.

Keywords: Preparation, Media, Bacterial growth, Jute retting

Location: Central Research Station, Dhaka

Start Date: 12-03-2005

End Date: 30-12-2007

Program Area: Pest Management

Commodity: Jute

Objectives:

To select appropriate media suitable for the growth of promising bacteria and to minimize the retting time as well as to improve the quality of fiber.

Methodology:

Firstly, jute plants (CVL-1) were grown in JAES, Jagir, Manikgonj. Plants were harvested at proper time and ribboned and dried in the sun. Promising bacteria were subcultured in potato dextrose agar (PDA), nutrient agar (NA), glucose peptone yeast (GPY) extract and beef liver-jute bark (BLJB) medium. Retting activities with promising microbes/ bacteria and designing of bacteria composing of different carbon and nitrogen sources were assessed. Promising bacteria were grown in different media at appropriate temperature and pH. Then the cultured bacteria were applied in jute retting using standard retting practices. Finally fiber quality was assessed.

Source of Information: Annual report 2005-2006, 2006-2007 and 2007-2008

Article Link (Online Journal):

Funding Source:

1. Government

Budget: Fifteen thousand only

Total Budget (Tk.) : Fifteen thousand only

Achievement/Findings:

In 2005, out of which ten strains, five strains grew well in Nutrient Agar media of which one strain (Micrococcus sp.1) was found to be the best retter which retted time was 6 days along with A grade fibre and other four strains (Micrococcus sp.4, Micrococcus sp.5, Bacillus sp.2 and Bacillus sp.3) were moderate. On the other hand, six strains grew well in Starch Agar media of which one strain (Clostridium sp.5) was found to be the best retter which retted time was 6 days along with A grade fibre and other three strains (Clostridium sp.1, Clostridium sp.2 and Clostridium sp.6) were moderate retters and rest of two were non retters. In 2006, out of the three bacterial strains, two strains (Bacillus sp. and Pseudomonas sp.) retted green jute ribbons at 10 and 14 days respectively grown in nutrient agar media but Bacillus sp. and Micrococcus sp. grown in peptone yeast extract medium and retted the jute ribbons at 13 days and 15 days respectively. The degrees of retting were ++++ and ++ for the Bacillus sp., Pseudomonas sp. Bacillus sp. and Micrococcus sp. respectively. In 2007, out of the five bacterial isolates viz. Micrococcus sp., Clostridium sp. and Bacillus sp. were grown on beef liver-jute bark (BLJB) media and retted green jute stem at 10-12 days respectively where Pseudomonas sp. and Bacillus sp, grew in nutrient agar media and retted green jute stem at 15 and 17 days respectively. The degrees of retting were +++ and ++ for the Micrococcus sp & Clostridium sp. and Bacillus sp. & Pseudomonas sp. respectively.

Knowledge Management: Report/Proceedings, Booklet/Leaflet