B. Roy
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh
Bikash C. Sarker
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh
M.N. Uddin
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh
Bizen C. Roy
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh
S. Jalal
Department of Agricultural Chemistry, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh
Aqueous extract, Allelochemical, Growth regulatory activity, Pulse crops
Department of Agricultural Chemistry in Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
Crop-Soil-Water Management
The experiment was conducted at the laboratory of Department of Agricultural Chemistry in Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh. The tested leaves were wood apple (Aegle marmelos), indian dillenia (Dillenia indica), papaya (Carica papaya), olive (Elaeocarpus floribundus), blackberry (Syzygium cumini), guava (Psidium guajava), banana (Musa sp), litchi (Litchi chinensis), mango (Mangifera indica) and jackfruit (Artocarpus heterophyllus). The treatments under investigation for the study of pulse seeds germination were water (T1), aqueous extract of mango leaves (T2), aqueous extract of blackberry leaves (T3), aqueous extract of jackfruit leaves (T4), aqueous extract of litchi leaves (T5), aqueous extract of wood apple leaves (T6), aqueous extract of indian dillenia leaves (T7), aqueous extract of papaya leaves (T8), aqueous extract of banana leaves (T9), aqueous extract of guava leaves (T10) and aqueous extract of olive leaves (T11). The freshly prepared aqueous extracts were applied on the seeds of selected pulse crops like BARIMash3, BINAMugS, Lentil2, and bean to observe their germination and growth performances. Aqueous extracts were prepared from fresh leaves as well as stems or whole plant of the selected fruit leaves species. For this, immature fruit leaves species vir, mango, banana, blackberry, jackfruit, litchi, wood apple, indian dillenia, papaya, guava and olive were collected randomly, selected and chopped and then it was made into paste by blender machine. Exactly 100 g of each fresh leaf was taken and cut into smaller pieces, then crushed using blender machine with the required amount of water and was then transferred to a 500 ml reagent bottle. Then 400 ml of water was added to it and kept for 72 h at room temperature (25±2°C) with regular interval of stirring. Therefore, after 72 h the aqueous slurry was filtered through Whatman filter paper No.1 and was taken in another 500 ml bottle. The filtrates of individual leaf extracts were stored and used for seed treatment and comprehensive study. Petridish experiments were performed for pulse crops such as BARIMush3, BINAMugS, Lentil2 and bean seeds for the observation of germination rate, shoot and root growths. For this experiment, clean petridish with two sheets of filter paper was placed. For the investigation of pulse seeds growth, 15 ml of each aqueous extract were taken in each petridish then 25 seeds of each pulse crop were placed on filter paper that was placed on petridish and each treatment was replicated into three (3) times. The Petridishes were kept in natural diffused light under laboratory conditions at room temperature (26±2°C). To moisten the filter paper, 5 ml water was applied if the filter paper was dried in control only distilled water was used. Germination and growth parameters were recorded everyday and it was continued until germination completed. Lengths of root and shoot were recorded after 7 days. The collected data were analyzed statistically and differences among means were compared by Duncan's Multiple Range Test (DMRT).
Journal of Science Technology (Dinajpur) Vol. 8: 95-102 (2010), ISSN 1994-0386
Journal