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Methods of Application of Trichoderma Harzianum for Controlling Fusarium Wilt and Late Blight of Tomato

Dr. Mahbuba Kaniz Hasna
Senior Scientific Officer
Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh

Dr. Hosne Ara Begum
Principal Scientific Officer
Plant Pathology Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh

Abstract/ Executive Summary:

Experiments were conducted in field station of Bangladesh Institute of Nuclear Agriculture (BINA) at Comilla and Rangpur during the Rabi season of 2010-2011 to determine suitable method(s) of application of Trichoderma harzianum for controlling fusarium wilt and late blight of tomato.The treatments were: T₁= Soil inoculation with T. harzianum, T₂= Seedling treatment with T. harzianum, T₃= Soil inoculation and seedling treatment with T. harzianum and T₄= Control (no treatment).. The treatments were: T₁= Soil inoculation with T. harzianum, T₂= Seedling treatment with T. harzianum, T₃= Soil inoculation and seedling treatment with T. harzianum and T₄= Control (no treatment). The present study indicates that soil inoculation with T. harzianum was more efficient than the other methods in reducing disease incidence of fusarium wilt and late blight of tomato.

Keywords: Fusarium wilt, Late blight, Tomato

Location: BINA sub station farm at Comilla and Rangpur

Start Date: 15-10-2010

End Date: 24-02-2011

Program Area: Pest Management

Commodity: Tomato

Objectives:

To determine suitable method(s) of application of Trichoderma harzianum for controlling fusarium wilt (Fusarium oxysporum f. sp. lycopersici) and late blight (Phytophthora infestans), the two major fungal diseases of tomato.

Methodology:

Experiments were conducted in field station of Bangladesh Institute of Nuclear Agriculture (BINA) at Comilla and Rangpur during the Rabi season of 2010-2011. The treatments were: T₁= Soil inoculation with T. harzianum, T₂= Seedling treatment with T. harzianum, T₃= Soil inoculation and seedling treatment with T. harzianum and T₄= Control (no treatment). The experiments were laid out in Randomized Complete Block Design with three replications. The unit plot size was 2m × 2m. Line to line and plant to plant spacing was 50 cm. The variety Pusaruby (susceptible to diseases) was used in the experiment.

The isolate of T. harzianum (coded as T₀₃₈) was obtained from Plant Pathology Division, BINA. For mass inoculum production of Trichoderma, moistened chickpea bran (10 g) was sterilized at 121⁰C with 15 Psi for 30 minutes. The substrate in an Erlenmeyer flask (500 ml) was inoculated with 10 mycelial discs (5 mm diameter) of T. harzianum previously grown on PDA. The flasks were incubated at 25± 1⁰ C for 15 days with intermittent hand shaking. For soil inoculation, the inoculum of T. harzianum was applied @ 5 g m^-2 at 3 days before transplanting. For seedling treatment, the inocula of T. harzianum grown in chickpea bran was suspended in water (5 g inocula in 1 L water) and sieved. The roots of the seedlings were soaked in the suspension for 1 hour before transplanting. Experiments were conducted in the soil naturally infested with F. oxysporum and P. infestans. Disease incidence was recorded at 40, 55 and 75 days after transplanting.

Source of Information: Bangladesh Journal of Nuclear Agriculture (BJNA): Vol.25 & 26

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Soil application of Trichoderma harzianum will be helpful for environment friendly management of fusarium wilt and late blight diseases of tomato in order to increase yield.

Knowledge Management: Journal