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Diagnosis of Control of Shrimp Diseases

Dr. Md. Shahadat Hossain
Senior Scientific Officer
Marine Fisheries & Technology Station, Bangladesh Fisheries Research Institute, Cox's Bazar

S. M. Zakiur Rahman
Scientific Officer
Marine Fisheries & Technology Station, Bangladesh Fisheries Research Institute, Cox's Bazar

Abstract/ Executive Summary:

A total of 45 water samples from hatchery larval tanks were collected and plated into agar plates containing thiosulphate citrate bile salt (TCBS) agar medium. Nine different antibiotic discs procured from pharmaceutical company were tested against 45 bacterial isolates. Ciprofloxacin was found to be the best antibiotic against different bacterial isolates and was followed by Erythromycin. A total of 13 different types of antibiotics (including banned antibiotics) were collected from hatchery operators and were tested against above mentioned bacterial isolates. Again Ciprofloxacin was found to be the best antibiotic against different bacterial isolates and was followed by Erythromycin (Em 55), Erythromycin (EE 65) and Oxytetracyclin (OTC). It is to mention that banned antibiotics like Furazolidon, Chloramphenicol, Nitroguran and Neomycin observed no inhibition.

Keywords: Shrimp, Disease, Health management

Location: Marine Fisheries & Technology Station, Cox's Bazar

Start Date: 01-07-2006

End Date: 30-06-2007

Program Area: Animal Health and Management

Commodity: Shrimp

Objectives:

To find out the sources of antibiotics used in the shrimp sector and effective antibiotics to prevent bacterial diseases in shrimp hatchery operation; and to study the prevalence of WSSV in captured broodstock, shrimp post-larvae and cultured shrimp by PCR techniques.

Methodology:

Samples of shrimp post-larvae and tank water were collected from different hatcheries at Kalatoli, Sonarpara and Taknaf, Cox’s Bazar and Satkhira, Khulna of southwest regions. Samples of post-larvae were macerated in sterile polythene sachet. Water samples were collected in a sterile appendorf tube from hatchery tank. The aliquots was plated onto agar plates containing Marine agar and thiosulphate citrate bile salt (TCBS) agar medium. Different bacterial isolates were grown in agar plates, where disc containing different antibiotics were put to see the antibiotic sensitivity of different isolates against different antibiotics.

Source of Information: Annual Research Program 2006-07. BFRI, Mymensingh.

Article Link (Online Journal):

Funding Source:

1. Government

Budget: Three lakh fifty thousand

Total Budget (Tk.) : Three lakh fifty thousand

Achievement/Findings:

Knowing the types of antibiotic sensitivity will be helpful to formulate guidelines (in line with the USFDA recommendations) of uses of antibiotics, which will be also helpful to disease control; knowing the status of a WSSV infection in broodstock will be helpful to select broodstock to produce virus free post-larvae and knowing the infection in post-larvae will be helpful to select virus free post-larvae to stock and to get better production; and overall work will help to create awareness among the shrimp sector clients.

Knowledge Management: Report/Proceedings