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Development of an Efficient in Vitro Regeneration System for Eggplant

M A Y Akhond
Senior Scientific Officer
Biotechnology Division, BARI

M A Muktadir
Scientific Officer
BARI

Abstract/ Executive Summary:

Eggplant (Solanum melongena L.) is one of the most popular vegetable in our country. Production of eggplant is associated with problems such as insect, disease and abiotic stresses. Development of resistant varieties through hybridization is difficult due to lack of natural source of resistance. Improvement of eggplant via genetic transformation is one of the important tools and regeneration is a prerequisite for transformation. The treatments of the experiment comprised of different combinations of 6-Benzylaminopurine (BAP) (0, 2.5 and 5.0 mg/l) and α- naphtaleneacetic acid (NAA) (0, 0.5, and 1.0 mg/l). Two different concentrations (1.0 mg/l and 2.0 mg/l) of Zeatin Riboside (ZR) and two concentrations (0.1 mg/l and 0.2 mg/l) of thidiazuron (TDZ) in combination with a single concentration (0.1 mg/l) of Indole-3-acetic acid IAA were also used in this experiment. The highest number of shoots per explant (6.65) was produced on MS medium supplemented with 2.0mg/l ZR with 0.1 mg/l IAA. A rooting experiment was carried out with 3 different levels (0.5, 1.0, 2.0 mg/l) of Indole Butaric Acid (IBA) where 2.0mg/l IBA supplemented medium was found to be the best. Plantlets with well developed roots and shoots were successfully transferred to soil.

Keywords: Eggplant, tissue culture, regeneration, in vitro

Location: Biotechnology laboratory, BARI, Gazipur

Start Date: 01-01-2007

End Date: 31-07-2008

Program Area: Variety and Species

Commodity: Brinjal

Objectives:

To develop an efficient plant regeneration protocol for locally adapted eggplant varieties aiming at future genetic transformation.

Methodology:

Variety	:	BARI-5
Media	:	MS supplemented with different growth regulators (NAA, 2,4-D, BAP, TDZ, Zeatin) with different concentrations.
Explant	:	Hypocotyl

Source of Information: Annual report, Biotechnology Division 2008-2009

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 1,00,000.00

Total Budget (Tk.) : 1,00,000.00

Achievement/Findings:

Hypocotyl segments of eggplant (Solanum melongena L.) var. BARI Begun-5 (Nayantara) were cultured on Murashige and Skoog (MS) media with different concentrations and combinations of growth regulators to establish a suitable regeneration protocol. Direct shoot organogenesis were observed in the treatments with ZR, TDZ and on MS medium without growth regulators. The highest number of shoots per explant (6.65) was produced on MS medium supplemented with 2.0mg/l ZR with 0.1 mg/l IAA. A rooting experiment was carried out with 3 different levels (0.5, 1.0, 2.0 mg/l) of Indole Butaric Acid (IBA) where 2.0mg/l IBA supplemented medium was found to be the best. Plantlets with well developed roots and shoots were successfully transferred to soil.

Knowledge Management: