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Determination of Immune Response of Newcastle Disease Virus Vaccines in Layer Chickens

Mr. Md. H. Chowdhury
MS Fellow
Department of Microbiology and Hygiene, Bangladesh Agricultural University,Mymensingh-2202Bangladesh

Abstract/ Executive Summary:

The study was conducted for the detection of persistence of Maternally derived antibody (MDA) as well as the comparative evaluation of antibody production of nine different NDV vaccines in layer chickens in the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh during the period from July to December 2008. A total of 55 layer chicks (ISA Brown breed) were divided into eleven groups each consisting of five birds of which odd number groups were vaccinated primarily with Nobilis® MA5+Clone 30, Avipro® ND-IB HB1, Cevac® BIL, Newcastle-Bronchitis Vaccine Fortdoge® and Avipro® ND LaSota vaccine respectively at day 5 of age and secondarily with Nobilis® ND Clone30, Avipro® ND LaSota, Cevac® New L, Newcastle Disease Vaccine Fortdoge® and Avipro® ND LaSota vaccine respectively at day 21 of age by single eye instillation and even number groups were vaccinated with the same vaccines respectively by double eye instillation following the same schedule. Again group 9 and group 10 were also vaccinated with RDV at 60 days of age through intramuscular route. Group 11 was kept as unvaccinated control. Sera samples were collected after 10 days of each vaccination and at day 5, 15, 20, 31 of age from unvaccinated control and subjected to HI test for the determination of antibody titres. It was observed that after primary vaccination the mean of HI titres of double eye vaccinated groups differed significantly (P<0.01). Overall analysis of mean of HI titres of double eye vaccinated groups revealed that there were significant increases (P<0.01) in HI titres in groups 8 (891.44±228.97) and 10 (861.66±140.21) compared to other groups. It was observed that secondary vaccination produced higher immune response compared to primary vaccination in case of all the vaccinated groups and double eye vaccination produced higher immune response compared to single eye vaccination in case of all the vaccinated groups. It was also observed that following vaccination with RDV in groups 9 and 10, HI titres increased significantly (P<0.01) which indicated that group 10 (1204.30±280.43 ) produced significantly higher antibody titres than group 9 (966.74±144). Maternal antibody was high (483.37±181.01) at day 5 of age and persisted to a minimal level (8.00±0.00) until the age of day 20 and almost disappears (≤4±0) at day 31 of age.

Keywords: Immune response, Newcastle disease, Vaccines, Layer chickens

Location: BAU,Mymensingh

Start Date: 00-07-2008

End Date: 00-12-2008

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

To determine the persistence of maternally derived antibody in the layer chicks
To compare the antibody titres of chickens vaccinated with eight different imported vaccines of lentogenic strains of NDV
To determine the antibody titres of chickens vaccinated with lentogenic and followed by mesogenic strain of NDV vaccines used in this study.

Methodology:

The whole experiment was conducted during the period from July to December 2008 in the Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. A total number of 55 day-old-chicks of ISA Brown breed with the history of parent stock vaccinated against Newcastle disease (ND) were obtained with the curtesy of C.P. Bangladesh Co. Ltd., Dhaka and carried to the experimental animal shed of the Department of Microbiology and Hygiene, BAU, Mymensingh. The birds were reared in litter supplying Nurish® poultry feed and water maintaining strict bio-security. Before vaccination the birds were divided into 11 groups, each group consists of five birds. The chicks of group 11 were kept as unvaccinated control. Nine lyophilized (freeze-dried) NDV vaccines were used in this study. These were (i) Nobilis® MA5+Clone 30 (Mass and clone 30 strain) of Intervet international (ii) Avipro® ND-IB HB1(Mass and B1 strain) of Lohmann Animal Health (iii) Cevac® BIL (Mass and B1 strain) of Ceva pharmaceuticals (iv) Newcastle- Bronchitis Vaccine Fortdoge® (Mass and B1 strain) of Fortdodge Animal Health (v) Nobilis® ND Clone30 (Clone 30 strain) of Intervet International (vi) Avipro® ND LaSota (LaSota strain) of Lohmann Animal Health (vii) Cevac® New L (LaSota strain) of Ceva pharmaceuticals (viii) Newcastle Disease Vaccine. Fortdoge (LaSota strain) of Fortdodge Animal Health and (ix) RDV (M strain) of DLS. Vaccines were obtained with the courtesy of C.P. Bangladesh Co. Ltd. These vaccines were stored at -20°C until used. Reference Newcastle disease virus. Newcastle disease virus (Komarov strain) used in microplate HI test for the determination of antibody titre were collected from the laboratory repository of the Department of Microbiology and Hygiene, BAU, Mymensingh was propagated to activate the virus into 10- day- old embryonated chicken eggs through allantoic cavity route . AF was collected aseptically using sterile syring and needle transferred to sterilecontainers. The presence of NDV in AF was tested by slide HA test and those manifesting HA- positive were collected (Cottral, 1978) as a source of reference ND virus and stored at -20°C until used. Vaccination schedule: A total of 55 layer chicks (ISA Brown) were divided into eleven groups such as 1,2,3,4, 5, 6, 7, 8, 9, 10 and 11 where each group consisting of five birds. Group 1, 3, 5. 7 and 9 were primarily vaccinated with Nobilis® MA5+Clone 30, Avipro® ND-IB HB1, Cevac® BIL, Newcastle-Bronchitis Vaccine Fortdoge® and Avipro® ND LaSota vaccine respectively at day 5 of age and secondarily with Nobilis® ND Clone30, Avipro ND LaSota, Cevac® New L, Newcastle Disease Vaccine Fortdoge® and Avipro® ND LaSota vaccine respectively at day 21 of age by single eye instillation and 2, 4, 6, 8 and 10 were vaccinated with the same vaccines respectively by double eye instillation following the same schedule. Again group 9 and group 10 were also vaccinated with RDV at 60 days of age through intramuscular route. Group 11 was kept as unvaccinated control. Sera samples were collected after 10 days of each vaccination and at day 5, 15, 20, 31 of age from unvaccinated control and subjected to HI test for the determination of antibody titres. Microlplate heamagglutination inhinition (HI) test: Micro-plate HI test was performed (Anon, 1971) to determine the antibody level of the sera samples collected from the chicks of different groups. The test was conducted using constant 4HA unit ND virus and diluted serum (β method). Statistical analysis:Statistical Package for social Sciences (SPSS) version 10.0 programme was used to analyze the data of the study. ANOVA Duncan’s Multiple Range Test (DMRT) and Paired ‘t’ test were performed to determine the significant difference

Source of Information: J. Bangladesh Agril. Univ. 7(2): 329–334, 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

From the present research it may be concluded that LaSota strain produced higher immune response than Clone 30 and B1 strain, Fortdose® and Avipro® vaccine produced higher immune response than all other vaccines and vaccination with lentogenic strains followed by mesogenic strain produced higher antibody titers used in this study.

Knowledge Management: Journal