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Effect of Different Doses of Ga3 Application at Primordia Initiation Stage on the Growth and Yield of Oyster Mushroom

Mr. R.R. Sarker
Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202 Bangladesh

Abstract/ Executive Summary:

Gibberellic acid (GA₃) was sprayed with eleven doses viz. 0, 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 ppm at the primordia initiation stage to evaluate its effect on the growth and yield performance of Oyster Mushroom. At 10 ppm level GA₃gave the highest economic yield and dry weight. Application of GA₃increased the fresh economic yields to about 30% and 34%, while 80% and 115% dry weights increase occurred compared to the control at first and second harvests, respectively. GA₃ showed a positive effect on number of effective fruiting body, stalk length, pileus diameter, biological yield, economic yield and dry economic yield. The result suggested that GA₃ at 10 ppm/packet would be the best possible concentration for production of Oyster Mushroom.

Keywords: Oyster mushroom, Gibberellic acid (GA3), Primordia initiation stage, Growth, Yield

Location: Bangladesh Institute of Nuclear Agriculture, Mymensingh

Start Date: 00-12-2010

End Date: 00-04-2011

Program Area: Crop-Soil-Water Management

Commodity: Mushroom

Objectives:

To find out optimum concentration of GA₃for maximizing growth and yield of mushroom.

Methodology:

The experiment was conducted in laboratory of the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh during the period from December 2010 to April, 2011. A plant growth regulator Gibberellic Acid (GA₃) was tested to see its effects on growth and yield of Oyster Mushroom by applying its eleven different levels of concentration at primordia initiation stage. The experiment was laid out in Completely Randomized Design with four replications. Eleven concentration levels were (i). 0 (control), (ii). 10 ppm, (iii). 20 ppm, (iv). 30 ppm, (v). 40 ppm, (vi). 50 ppm, (vii). 60 ppm, (viii). 70 ppm, (ix). 80 ppm,(x). 90 ppm and (xi). 100 ppm. There were a total of 44 spawn packets of mushroom. The variety was Oyster mushroom for this experiment.The 500 g spawn packet was made with the components of i.e. 192.5 g saw dust, 87.5 g wheat bran, 10 g rice husk, and 1g CaCO₃ which were mixed and moisture was adjusted at 50% by adding water. The mixture was filled into 7′′× 10′′ poly propylene bags. A hole (about 5 cm) was made with a sharp end stick at the center for space to put the inoculums. It was plugged with cottons and covered with brown paper placing a rubber band to hold it on place. In such a way 44 prepared packets were sterilized for 2 hours at 120°C and 1.5 kg/cm² pressure and kept 24 hours for cooling. Then one teaspoon of mother culture media containing mycelia supplied from Horticulture Center, Kewatkhali, Mymensingh was placed aseptically through the hole of each packet separately. The packets were plugged with cottons and covered with brown paper placing a rubber band to hold in place. The packets were then marked treatment wise and kept on the rack in an incubation room at 20-25°C under 65-67% relative humidity. After 18-28 days of incubation, when colonization was completed the spawn packets were used for the cultivation of Oyster mushroom.The spawn packets were opened by ‘D’ shaped cut on the shoulder side. The open surface of substrate was scraped slightly with a blade for removing the thin whitish mycelial layer. The spawn packets were soaked in water for half an hour and squeezed to remove excess water. The packets were then placed randomly side by side on the rack in the culture house. Eleven different levels of GA₃ were sprayed on the packets at primordia initiation stage. Proper care was taken in case of disinfecting the culture room. The relative humidity of 70- 80% and the temperature of 20-30°C were maintained by watering twice a day in the room.The data obtained from the experiment for every parameter were analyzed statistically using MSTAT-C computer program. The means were compared following Duncan’s multiple Range Test (Gomez and Gomez, 1984).

Source of Information: J. Bangladesh Agril. Univ. 11(1): 5–10, 2013, ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The experiment found that the highest biological and economic yield and also dry weight were obtained when 10 ppm GA₃was applied on primordia initiation stage. At 10 ppm level GA₃gave the highest economic yield of 87.31g and 43.25 g, and dry weight of 6.72 g and 3.48g at first and second harvest, respectively. The lowest economic yield and dry weight were recorded in 50 ppm at first harvest and at second harvest it was recorded in 100 ppm. GA₃ showed a positive effect on number of effective fruiting body, stalk length, biological yield, economic yield and dry economic yield. An appropriate concentration of GA₃would maximize the yield which would benefit the growers and pave the way in marketing it at a lower price.

Knowledge Management: Journal