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Identification of Iron Rich Rice Genotypes in Bangladesh Using Chemical Analysis

Dr. Lutful Hassan
Professor
Department of Genetics and Plant Breeding, Bangladesh agricultural University, Mymensingh-2202Bangladesh

S. N. Islam
Department of Genetics and Plant Breeding, Bangladesh agricultural University, Mymensingh-2202, Bangladesh

S. N. Begum
Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh

G. S. Jahan
Department of Genetics and Plant Breeding, Bangladesh agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

An experiment was carried out to identify the iron rich rice genotypes. Total fifty two rice genotypes were used for this purpose. Iron (Fe) content of rice grain samples was determined by using Atomic Absorption Spectrophotometer (AAS). In this process the samples were digested by the application of di-acid mixture which includes nitric acid (HNO₃): and perchloric acid (HClO₄) in 2:1 ratio. Iron content was estimated in the aliquot of seed extract by using Atomic Absorption Spectrophotometer (AAS) at 248.33 nm. Iron concentration ranged from 1.32 ppm (Jota Balam) to 100.45 ppm (Lal Gotal). Among the 52 genotypes local landraces had showed the highest Fe content. In this experiment, ANOVA table revealed that significant variation was found among the genotypes for the iron concentration. This suggested that there were inherent genetic differences among the genotypes. Thus, local landraces can be a good source for biofortification of popular rice cultivars using different breeding methods.

Keywords: Iron, Rice genotypes, Atomic Absorption Spectrophotometer (AAS), Biofortification

Location: Mymensingh

Start Date: 00-06-2012

End Date: 00-01-2013

Program Area: Variety and Species

Commodity: Rice

Objectives:

To identify the iron rich rice genotypes among the existing Bangladeshi genotypes.

Methodology:

The experiment was conducted at the experimental laboratory of Department of Agricultural Chemistry, Bangladesh Agricultural University and Soil Science Division of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, during June 2012 to January 2013.A total of fifty two (52) rice genotypes, consisting of forty (40) landraces representing southern and northern part of Bangladesh, five (5) high yielding varieties (HYV), three (3) mega varieties and four (4) advanced lines were collected to carry out the objectives of this research work. Processing of seeds of fifty two rice genotypes for chemical analysis was done by dehusking and grinding. Before analyzing the rice samples for iron estimation, the seeds of all the 52 accessions were subjected to dehusking and grinding. The collected rice seeds were oven dried at 55⁰ C temperatures for 48 hours to facilitate the dehusking process. The dehusked seeds were then grinded to make it powder with the help of a mechanical grinder. The rice grain powder of each genotypes was then stored in a polythene bag. From there one gram (1g) of the rice grain powder for each genotypes was weighed separately for the further use. Iron (Fe) content of rice grain samples was determined by using Atomic Absorption Spectrophotometer (AAS) as stated by Lindsay and Novell (1978). In this process the samples were digested by the application of di-acid mixture (Bhatia and Khetarpaul, 2012; Shaibur et al., 2010) which includes nitric acid (HNO₃): and perchloric acid (HClO₄) in 2:1 ratio.

Source of Information: J. Bangladesh Agril. Univ. 11(1): 73–78, 2013

Article Link (Online Journal):

Funding Source:

1. World Bank	Budget:
2. Bangladesh Agricultural Research Council	Budget:

Total Budget (Tk.) :

Achievement/Findings:

The local landrace, Lal Gotal had the highest (100.45 ppm) iron concentration where as Jota Balam had the lowest amount of iron concentration (1.32 ppm). Among the commercial cultivated varieties; BR 11, BRRI dhan 29 and BRRI dhan 57 have the considerably higher amount of iron content, so it can be proposed to eat these rice varieties to cope with iron deficiencies. Furthermore, among the screened materials, the genotypes having higher iron content can be used as a breeding material for biofortification process in future.

Knowledge Management: Journal