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Isolation and Identification of Avibacterium Paragallinarum, the Causal Agent of Infectious Coryza (ic) from Layer Chickens in Bangladesh

Dr. Md. Mokbul Hossain
Professor
Department of Pathology, Bangladesh Agricultural UniversityMymensingh-2202Bangladesh

Abstract/ Executive Summary:

This study was carried out for the isolation and identification of Avibacterium paragallinarum, the etiological agent of infectious coryza (IC). Pathological changes were also investigated that occurred in organs in layer chickens obtained from some selected areas of Bangladesh. A nasal swabs (n=30) from dead chickens and four swabs from live chickens were collected aseptically. The organisms from swabs were cultured in different media, stained, and followed by sugar fermentation and biochemical tests for identification of causal agent. Histopathology of the affected tissues was also carried out. Swabs from nasal passage (n=30) of dead birds died of other diseases cultured but no A. paragallinarum was isolated and identified. Two out of four suspected clinical cases of IC was confirmed by isolation of A. paragallinarum. The clinical signs of all four cases were nasal discharge; conjunctivitis with swelling of the sinuses, face and wattles; decreased feed and water intake with reduced egg production. At necropsy, hemorrhages were in mucous membrane of nasal passage and trachea. Histopathology of the nasal septum showed acanthosis, congestion, mucous glandular cell hyperplasia, hyperplasia of nasal sinus and parakeratosis. Further studies involving serological and molecular identification of the etiological agent of IC are warranted.

Keywords: Infectious coryza, Avibacterium paragallinarum, Isolation, Histopathology

Location: Dept. of Pathology

Start Date: 01-06-2011

End Date: 30-05-2012

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

i) To carry out for the isolation and identification of Avibacterium paragallinarum, the etiological agent of infectious coryza (IC). Pathological changes were also investigated that occurred in organs in layer chickens obtained from some selected areas of Bangladesh, ii) To study involving serological and molecular identification of the etiological agent of IC are warranted.

Methodology:

The present study was divided in three phases. First phase included, isolation and identification of Staphylococcus aureus, that supplied nicotinamide adenine dinucleotide (NAD) and acted as feeder colony for Aviobacterium paragallinarum, the second phase included isolation and identification of vibacterium paragallinarum and the third phase included pathological study of affected organs. The study was conducted during June 2011 to May 2012. For the isolation of Staphylococcus aureus, 10 nasal swab samples from nasal passages of 10 live layer chickens were collected aseptically from Bangladesh Agricultural University (BAU) poultry farm.

Source of Information: J. Bangladesh Agril. Univ. 11(1): 87–96, 2013, ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Histopathology of the nasal septum showed acanthosis, congestion, mucous glandular cell hyperplasia, hyperplasia of nasal sinus and parakeratosis.

Knowledge Management: Journal